GENETIC VARIABILITY OF BLACCURRANT VARIETIES (RIBES NIGRUM) IN BELARUS
Abstract
The study of genetic variability of Ribes nigrum varieties grown in Belarus with using of 7 microsatellite markers showed that modern Belarussian varieties have close genetic relationship with foreign varieties. The numbers of alleles in the studied loci were from 3 to 11. The average number of unique genotype among 60 samples was 16.3. The discrimination power of markers varied from 0.5 to 0.87 and the mean value was 0.71. All markers possess rather high diagnostic value and allow to identify black currant varieties at the molecular level and can be recommended for DNA-identification of those cultures.
About the Authors
O. A. Mezhnina
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Junior researcher
Belarus
Junior researcher
O. Yu. Urbanovich
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
D. Sc. (Biol.), Head of the laboratory
Belarus
D. Sc. (Biol.), Head of the laboratory
References
1. Shultheis, L. M. and Donoghue, M. J. (2004),“Molecular phylogeny and biogeography of Ribes (Grossulariaceae), with an emphasis on gooseberries (subg. Grossularia)”, Systematic Botany, vol. 29, pp. 77–96.
2. Cronquist, A. (1988), “The Evolution and Classification of Flowering Plants”, New York, US.
3. Sinnot, Q. P. (1985), “A reversion of Ribes L. Subg. Grossularia (Mill.) pers. sect. Gros-sularia (Mill. Nutt. (Gros- sulariaceae) in North America”, Rhodora, vol. 87, pp. 198–286.
4. Brennan, R. M. (2008), “Currants and Gooseberries”, in Hancock, J. F. (ed.), Temperate Fruit Crop Breeding: Germplasm to Genomics, Springer, The Netherlands, NL, pp. 177–196.
5. Korbin, M., Kuras, A. and Zurawicz, E. (2002), “Fruit plant germplasm characterisation using molecular markers generated in RAPD and ISSR-PCR”, Molecular Biology Letters, vol. 7, pp. 785–794.
6. Lanham, P. G., Korycinska, A. and Brennan, R. M. (2000), “Genetic diversity within a secondary pool for Ribes nigrum L. revealed by RAPD and ISSR markers”, Journal of Horticultural Science and Biotechnology, vol. 75, pp. 371–375.
7. Lanham, P., Brennan, R. M. and McNicol, R. J. (1995). “Fingerprinting of blackcurrant (Ribes nigrum L.) cultivars using RAPD analyses”, Theoretical and Applied Genetics, vol. 90, pp. 166–172.
8. Brennan, R. M. and Gordon, S. L. (2002), “Future perspectives in black currant breeding”, Acta Horticulturae, vol. 585, pp. 39–45.
9. De Mattia, F., Grassi, F., Imazio, S. and Labra, M. (2008), “Chloroplast and nuclear DNA markers to characterize cultivated and spontaneous Ribes”, Plant Biosystems, vol. 142, no. 2, pp. 204–212.
10. Lanham, P. G. and Brennan, R. M. (1999), “Genetic characterization of gooseberry (Ribes subgenus Grossularia) germplasm using RAPD, ISSR and AFLP markers”, Journal of Horticultural Science, vol. 74, pp. 361–366.
11. Brennan, R. M., Jorgensen, L., Hackett, C., Woodhead, M., Gordon, S. L. and Russel, J. (2008), “The development of a genetic linkage map of blackcurrant (Ribes nigrum L.) and the identification of regions associated with key fruit quality and agronomic traits”, Euphytica, vol. 161, pp. 19–34.
12. Russell, J. R., Bayer, M., Booth, C., Cardle, L., Hackett, C. A., Hedley, P. E., Jorgensen, L., Morris, J. A. and Bren- nan, R. M. (2011), “Identification, utilisation and mapping of novel transcriptome-based markers from blackcurrant (Ribes nigrum)”, BMC Plant Biology, vol. 10, pp. 147–157.
13. Brennan, R. M., Jorgensen, L., Woodhead, M. and Russell, J. (2002), “Development and characterisation of SSR markers in Ribes species”, Molecular Ecology Notes, vol. 2, pp. 327–330.
14. Kalia, R. K., Rai, M. K., Kalia, S., Singh, R. and Ghawaa, A. K. (2011), “Microsatellite markers: an overview of the recent progress in plants”, Euphytica, vol. 177, no. 3, pp. 309–314.
15. Palmieri, L., Grando, M. S., Sordo, M., Grisenti, M., Martens, S. and Giongo, L. (2013), “Establishment of molecular markers for germplasm management in a worldwide provenance Ribes spp. collection”, POJ, vol. 6, no. 3, pp. 165–174.
16. Antonius, K., Karhu, S., Kaldmae, H., Lacis, G., Rugenius, R., Baniulis, D., Sasnauskas, A., Schulte, E., Kuras, A., Korbin, M., Gunnarsson, A., Werlemark, G., Ryliskis, D., Todam-Andersen, T., Kokk, L. and Jarve, K. (2012), “Development of the Northern European Ribes core collection based on a microsatellite (SSR) marker diversity analysis”, Plant Genetic Resources, vol. 10, no. 1, pp. 70–73.
17. Cavanna, M., Marioni, D. T., Beccaro, G. L. and Bounous, G. (2009), “Microsatellite-based evaluation of Ribes spp. germplasm”, Genome, vol. 52, pp. 839–848.
18. Pikunova, А. V., Knyazev, S. D., Bakhotskaya, А. Yu. and Kochumova, А. А. (2015), “Microsatellite loci polymorphism in Russian black currant (Ribes nigrum L.) varieties from collection of All-Russian Research Institute of Breading Fruit Crops”, Agricultural Biology, vol. 50, no. 1, pp. 46–54.
19. Van de Peer, Y. and R. de Wachter (1993), “TREECON: a software package for the construction and drawing of evolutionary trees”, Computer Applications in the Biosciences, vol. 9, pp. 177–182.
20. Nei, M., Li and W. H. (1979), “Mathematical model for studying genetic variation in terms of restriction endonucleases”, Proceedings of the National Academy of Sciences of the United States of America, vol. 76, pp. 5269–5273.
21. Kloosterman, A. D., Budowle, B. and Daselaar, P. (1993), “PCR-amplification and detection of the human DIS 80 VNTR locus. Amplification condition, population genetics and application in forensic analysis”, International Journal of Legal Medicine, vol. 105, pp. 257–264.
22. Sipahi, H. (2011), “Genetic screening of Turkish barley genotypes using simple sequence repeat markers”, Journal of Cell and Molecular Biology, vol. 9, no. 2, pp. 19–26.
23. Clerc, V. L., Bazante, F., Baril, C., Guiard. J. and Zhang, D. (2005), “Assessing temporal changes in genetic diversity of maize varieties using microsatellite markers”, Theoretical and Applied Genetics, vol. 110, pp. 294–302.
24. Xie, H., Sui, Y., Chang, F.Q., Xu, Y. and Ma, R. C. (2006), “SSR allelic variation in almond (Prunus dulcis Mill.)”, Theoretical and Applied Genetics, vol. 112, pp. 366–372.
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