THE CREATION OF TRANSGENIC PLANTS NICOTIANA TABACUM WITH GENE NDB2 ARABIDOPSIS THALIANA TO STUDY RESPONSE TO STRESS
Abstract
Transgenic tobacco plants that express the ndb2 gene were obtained to study the influence of changed level of the ndb2 gene (NAD(P)H dehydrogenase B2, EC 1.6.5.2) expression on the activity of the proteins of mitochondrial localization and stress resistance of plants. The ndb2 gene of 1749 bp was isolated from Arabidopsis thaliana total messenger RNA using RT-PCR. This gene was cloned into the pBI121 plasmid. The pBI121 _ NDB2 vector construction containing the ndb2 gene under the control of the 35S CaMV promoter was created on the basis of the pBI121 plasmid. The pBI121_NDB2 efficiency for transformation was demonstrated. The ndb2 gene integration into the tobacco genome was confirmed experimentally. The tobacco lines expressing target ndb2 gene were obtained.
About the Authors
D. V. Sauchyn
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Biol.), Researcher
Belarus
Ph. D. (Biol.), Researcher
P. V. Kuzmitskaya
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
Researcher
Belarus
Researcher
O. Yu. Urbanovich
Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
Belarus
D. Sc. (Biol.), the Head of the laboratory
Belarus
D. Sc. (Biol.), the Head of the laboratory
G. B. Borovskii
Siberian Institute of Plant Physiology and Biochemistry Siberian Branch of Russian Academy of Sciences
Russian Federation
Ph. D. (Biol.), Researcher
Russian Federation
Ph. D. (Biol.), Researcher
I. V. Fedoseeva
Siberian Institute of Plant Physiology and Biochemistry Siberian Branch of Russian Academy of Sciences
Russian Federation
D. Sc. (Biol.), Professor, Chief researcher
Russian Federation
D. Sc. (Biol.), Professor, Chief researcher
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