SELECTION OF BUTANOL-RESISTANT MUTANTS OF CLOSTRIDIUM ACETOBUTYLICUM S1
Abstract
Conditions were optimized for chemical mutagenesis of bacteria C. acetobutylicum S1. It was shown that treatment of bacterial culture pre-grown for 8 hours in standard synthetic MSS medium, with N-methyl-N′-nitro-N-nitrosoguanidine at concentration 50 μg/ml in citrate buffer (pH = 5.5) during 60 min at 25 °C allowed to select mutantvariants stably inheriting the trait of resistance to 2.5 % butanol. Analysis of fatty acid composition of cytoplasmic membrane has revealed presence of 2-hexylcyclopropane octanoic acid in mutant distinguished by elevated butanol production.
About the Authors
E. V. Bolotnik
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Biol.), Deputy Director
Belarus
Ph. D. (Biol.), Deputy Director
A. A. Chereshnev
Belarusian State University
Belarus
Graduate student
Belarus
Graduate student
M. A. Titok
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
D. Sc. (Biol.), Professor, Chief researcher
Belarus
D. Sc. (Biol.), Professor, Chief researcher
E. I. Kolomiets
State Scientific-Production Association “Chemical Synthesis and Biotechnology”
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Corresponding Member, D. Sc. (Biol.), General Director of State Scientific-Production Association “Chemical Synthesis and Biotechnology” – Director of Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Corresponding Member, D. Sc. (Biol.), General Director of State Scientific-Production Association “Chemical Synthesis and Biotechnology” – Director of Institute of Microbiology of the National Academy of Sciences of Belarus
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