SYNTHESIS OF FLUDARABINE 5′-MONOPHOSPHATE USING BACTERIAL RECOMBINANT ENZYMES
Abstract
The scheme of enzymatic preparation of fludarabine-5′-monophosphate from 2-fluoroadenine and cyclocytidine as a donor of arabinose fragment was developed and experimentally proved. Acetylphosphate was used as a donor of phosphate group. Cytidine deaminase, uridine phosphorylase, purine nucleoside phosphorylase and deoxynucleoside kinase recovered from earlier designed strains of Escherichia coli were used as biocatalysts. The synthesized fludarabine-5′-monophosphate is planned for using as a pro-drug in enzymatic pro-medicinal approach to therapy of cancer.
About the Authors
A. I. Beresnev
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Biol.), Senior scientific researcher. Institute of Microbiology
Belarus
Ph. D. (Biol.), Senior scientific researcher. Institute of Microbiology
A. N. Rymko
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Biol.), Senior scientific researcher. Institute of Microbiology
Belarus
Ph. D. (Biol.), Senior scientific researcher. Institute of Microbiology
L. A. Eroshevskaya
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Biol.), Lead scientific researcher. Institute of Microbiology
Belarus
Ph. D. (Biol.), Lead scientific researcher. Institute of Microbiology
S. V. Kvach
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Ph. D. (Biol.), Lead scientific researcher. Institute of Microbiology
Belarus
Ph. D. (Biol.), Lead scientific researcher. Institute of Microbiology
E. I. Kvasyuk
International State Ecological Institute named after A. D. Sakharov of the Belarusian State University
Belarus
D. Sc. (Chem.), Professor
Belarus
D. Sc. (Chem.), Professor
A. I. Zinchenko
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Corresponding Member, D. Sc. (Biol.), Professor, Head of the laboratory
Belarus
Corresponding Member, D. Sc. (Biol.), Professor, Head of the laboratory
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