GENETIC POLYMORPHISM AND APPLE MOSAIC VIRUS PREVALENCE IN THE GARDENS OF BELARUS
Abstract
Apple trees of different cultivars growing in Belarus were tested using RT-PCR for Apple mosaic virus, an important economically and common pathogens in commercial orchards. Viral infected apple trees (1.5 %) were detected in the modern horticultural plantations and were not found among the old trees aged more than 50 years. The fragments of ApMV coat protein gene were cloned and sequenced. The analysis of their nucleotide sequences showed that the identity between them ranged from 98.9 to 99.6 %. The analysis of evolutionary relationships of nucleotide sequences obtained in this study and their homologs from the GenBank database revealed that the similarity between the viral genotypes was observed both between the isolates from various plants growing in the same geographical region, as well as between the isolates obtained from the same plant species, growing in different geographic regions. This picture was probably formed as a result of two methods of apple mosaic virus propagation: vegetative and mechanical.
About the Authors
P. V. KUZMITSKAYA
Institute of Genetics and Cytology of National Academy of Belarus
Belarus
Belarus
O. Yu. URBANOVICH
Institute of Genetics and Cytology of National Academy of Belarus
Belarus
Belarus
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