Inhibition of functional activity of rat liver mitochondria under acute intoxication by carbon tetrachloride
Abstract
Acute carbon tetrachloride-induced rat liver damage (4 g/kg) after 24 h was accompanied by a significant reduction in succinate- and glutamate-dependent mitochondrial respiration rate in state 3 (by 65 %, and by 50 %, respectively). The acceptor control ratio and respiration control ratio approached to 1, reflecting the loss of respiration control. The mitochondrial alterations were associated with oxidation of intramitochondrial GSH, the inhibition of succinate dehydrogenase (complex II) and the rise of blood plasma nitric oxide level. Melatonin administration under CCl4-induced intoxication (three times at doses of 10 mg/kg) did not cause a pronounced recovery of mitochondrial functional activity, but prevented an increase in nitric oxide level in the blood plasma of intoxicated animals and enhanced the rate of succinate oxidation in state 3 by 30 % (p < 0.05).
References
1. Duchen, M. R. Mitochondria in health and disease: perspectives on a new mitochondrial biology / M. R. Duchen // Mol. Aspects of Med. - 2004. - Vol. 25. - P. 365-451.
2. Muller, F. L. Complex III releases superoxide to both sides of the inner mitochondrial membrane / F. L. Muller, Y. Liu, H. Van Remmen // J. Biol. Chem. - 2004. - Vol. 279, N 47. - P. 49064-49073.
3. Control of mitochondrial redox balance and cellular defense against oxidative damage by mitochondrial NADP+-dependent isocitrate dehydrogenase / S. H. Jo [et al.] // J. Biol. Chem. - 2001. - Vol. 276, N 19. - P. 16168-16176.
4. Mechanisms of hepatotoxicity / H. Jaeschke [et al.] // Toxicol. Sci. - 2002. - Vol. 65, N 2. - P. 166-176.
5. Martin, E. J. Mitochondrial dysfunction is an early manifestation of 1,1-dichloroethylene-induced hepatotoxicity in mice / E. J. Martin, W. J. Racz, P. G. Forkert // J. Pharmacol. Exp. Ther. - 2003. - Vol. 304, N 1. - P. 121-129.
6. Suomalainen, A. Biomarkers for mitochondrial respiratory chain disorders / A. Suomalainen // J. Inherit. Metab. Dis. -2011. - Vol. 34, N 2. - P. 277-282.
7. Fosslien, E. Mitochondrial medicine-molecular pathology of defective oxidative phosphorylation / E. Fosslien // Ann. Clin. Lab. Sci. - 2001. - Vol. 31, N 1. - P. 25-67.
8. Weber, L. W. Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model / L. W. Weber, M. Boll, A. Stampfl // Crit. Rev. Toxicol. - 2003. - Vol. 33. - P. 105-136.
9. Protective effects of melatonin against carbon tetrachloride hepatotoxicity in rats / L. B. Zavodnik [et al.] // Cell Bio-chem. Funct. - 2005. - Vol. 23. - P. 353-359.
10. Rat liver mitochondrial damage under acute or chronic carbon tetrachloride-induced intoxication: protection by melatonin and cranberry flavonoids / V. Cheshchevik [et al.] // Toxicol. and Appl. Pharmacol. - 2012. - Vol. 261. - P. 271-279.
11. (Z)-5-(4-methoxybenzylidene)thiazolidine-2,4-dione protects rats from carbon tetrachloride-induced liver injury and fibrogenesis / Z. Z. Chen [et al.] // World J. Gastroenterol. 2012. - Vol. 18. - P. 655-661.
12. Basu, S. Carbon tetrachloride-induced lipid peroxidation: eicosanoid formation and their regulation by antioxidant nutrients / S. Basu // Toxicology. - 2003. - Vol. 189. - P. 113-127.
13. Jaeschke, H. Intracellular signaling mechanisms of acetaminophen-induced liver cell death / H. Jaeschke, M. L. Bajt // Toxicol. Sci. - 2006. - Vol. 89, N 1. - P. 31-41.
14. Hardeland, R. Kynuramines, metabolites of melatonin and other indoles: the resurrection of an almost forgotten class of biogenic amines / R. Hardeland, D. X. Tan, R. J. Reiter // J. Pineal Res. - 2009. - Vol. 47. - P. 109-126.
15. Sun, H. Melatonin: pharmacological aspects and clinical trends / H. Sun, F. F. Huang, S. Qu // Lipids Health Dis. -2015. - Vol. 14. - P. 75.
16. Mitochondrial catastrophe during doxorubicin-induced cardiotoxicity: a review of the protective role of melatonin / J. Govender [et al.] // J. Pineal Res. - 2014. - Vol. 57. - P. 367-380.
17. Melatonin prevents oxidative damage induced by maternal ethanol administration and reduces homocysteine in the cerebellum of rat pups / F. Bagheri [et al.] // Behav. Brain Res. - 2015. - Vol. 287. - P. 215-225.
18. Constitutive and UV-induced metabolism of melatonin in keratinocytes and cell-free systems / T. W. Fischer [et al.] // FASEB J. - 2006. - Vol. 20. - P. E897-E907.
19. Corrections by melatonin of liver mitochondrial disorders under diabetes and acute intoxication in rats / V. Cheschevik [et al.] // Cell Biochem. Funct. - 2011. - Vol. 29. - P. 481-488.
20. Melatonin: pharmacological aspects and clinical trends / E. R. Rios [et al.] // Int. J. Neurosci. - 2010. - Vol. 120. -P. 583-590.
21. Identification of highly elevated levels of melatonin in bone marrow: its origin and significance / D. X. Tan [et al.] // Biochim. Biophys. Acta. - 1999. - Vol. 1472. - P. 206-214.
22. Evidence of melatonin synthesis by human lymphocytes and its physiological significance: possible role as intracrine, autocrine, and/or paracrine substance / A. Carrillo-Vico [et al.] // FASEB J. - 2004. - Vol. 18. - P. 537-539.
23. Западнюк, И. П. // Лабораторные животные, их разведение, содержание и использование в эксперименте / И. П. Западнюк, В. И. Западнюк, Е. А. Захария. - Киев: Медизд., 1962. - С. 260-261.
24. Johnson, D. // Methods in Enzymology / D. Johnson, H. A. Lardy; R. Estabrook, M. E. Pullmam (eds.). - N. Y., London: Acad. Press, 1967. - Vol. 10. - P. 94-96.
25. Oxygen-related processes in red blood cells exposed to tert-butyl hydroperoxide / I. K. Dremza [et al.] // Redox Rep. -2006. - Vol. 11. - P. 185-192.
26. Martinez, J. I. A sensitive fluorimetric microassay for the determination of glutathione peroxidase activity. Application to human blood platelets / J. I. Martinez, J. M. Launay, C. Dreux // Anal. Biochem. - 1979. - Vol. 98. - P. 154-159.
27. Nulton-Persson, A. C. Modulation of mitochondrial function by hydrogen peroxide / A. C. Nulton-Persson, L. I. Szweda // J. Biol. Chem. - 2001. - Vol. 276. - P. 23357-23361.
28. Ellman, G. L. Tissue sulfhydryl groups / G. L. Ellman // Arch. Biochem. Biophys. - 1959. - Vol. 82. - P. 70-77.
29. Thiol groups in proteins as endogenous reductants to determine glutathione-protein mixed disulphides in biological systems / R. Rossi [et al.] // Biochim. Biophys. Acta. - 1995. - Vol. 1243. - P. 230-238.
30. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids / L. C. Green [et al.] // Anal. Biochem. - 1982. -Vol. 126. - P. 131-138.
31. Protein measurement with the Folin phenol reagent / O. H. Lowry [et al.] // J. Biol. Chem. - 1951. - Vol. 193. -P. 265-275.
32. Smuckler, E. A. Structural and functional changes in acute liver injury / E. A. Smuckler // Environ. Health Perspect. -1976. - Vol. 15. - P. 13-25.
33. Padma, P. Protective effect of Phyllanthus fraternus against carbon tetrachloride-induced mitochondrial dysfunction / P. Padma, O. H. Setty // Life Sci. - 1999. - Vol. 64, N 25. - P. 2411-2417.
34. Melatonin directly scavenges free radicals generated in red blood cells and a cell-free system: Chemiluminescence measurements and theoretical calculations / I. B. Zavodnik [et al.] // Life Sci. - 2006. - Vol. 79. - Vol. 70. - P. 391-400.
35. The chemistry of melatonin’s interaction with reactive species / M. Allegra [et al.] // J. Pineal Res. - 2003. - Vol. 34. -P. 1-10.
36. Metabolism of melatonin by cytochrome P-450s in rat liver mitochondria and microsomes / I. Semak [et al.] // J. Pineal Res. - 2008. - Vol. 45. - P. 515-523.
37. Кузнецова, Е. И. Влияние мелатонина и его производных на активность дыхательных комплексов различных органов крыс in vitro / Е. И. Кузнецова, И. В. Семак // Новости мед.-биол. наук. - 2014. - № 1. - C. 38-42.
38. Oxidative damage of rat liver mitochondria during exposure to t-butyl hydroperoxide. Role of Ca2+-ions in oxidative processes / I. B. Zavodnik [et al.] // Life Sci. - 2013. - Vol. 92. - P. 1110-1117.
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