Construction of Escherichia coli strain, producing di-adenylate cyclase and its application for cyclic di-AMP synthesis
Abstract
A new recombinant strain of Escherichia coli pBtdac, producing diadenylate cyclase Bacillus thuringiensis has been constructed. The amount of the diadenylate cyclase produced by the strain is 32 mg per 1 L of culture broth. The substrate specificity and stability of the produced enzyme has been studied. Application of the purified diadenylate cyclase for preparative (600 mg) cyclic di-AMP synthesis has been demonstrated.
About the Authors
I. S. Kazlovskij
Sakharov International State Ecological University
Belarus
Belarus
D. S. Radevich
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
A. N. Rymko
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
A. S. Shchokolova
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
S. V. Kvach
Institute of Microbiology of the National Academy of Sciences of Belarus
Belarus
Belarus
A. I. Zinchenko
Sakharov International State Ecological University
Belarus
Belarus
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