Accumulation of 5-aminolevulinic acid, chlorophyll and carotenoids in transgenic tobacco plants with yeast ALA-synthase gene

Three lines of transgenic tobacco plants (Nicotiana tabacum L. cv. Petit Havana SR1) expressing recombinant Saccharomyces cerevisiae ALA-synthase gene that contains an information for targeting peptide providing penetration of the enzyme to mitochondria (lines 1.1, 1.3, 1.7) and one line expressing gene of the ALA-synthase that is synthesized and localized in cytoplasm were obtained (line 2.1). All transgenic plants possessed by increased ALA-synthesizing ability and were characterized by improved growth parameters. Higher content of photosynthetic pigments - chlorophyll and carotenoids as compared to wild-type plants was also registered in plants of the line 2.1. The received transgenic plants can serve as model for research of a role of endogenous ALA in providing photosynthetic activity of chloroplast and forming of plant stress resistance that will be used for creating the biotechnologically improved cultivars.

табак, трансгенные растения, 5-аминолевулиновая кислота, АЛК-синтетаза, АЛК-синтезиру-ющая способность, хлорофилл, каротиноиды, tobacco, transgenic plants, 5-aminolevulinic acid, ALA-synthase, ALA-synthesizing ability, chlorophyll and carotenoids

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