vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2026-71-1-34-43vestib-995Research ArticleСтатьиАнализ селекционного генофонда озимой мягкой пшеницы (Triticum aestivum L.) по генам, ассоциированным с устойчивостью к предуборочному прорастаниюAnalysis of the breeding gene pool of winter wheat (Triticum aestivum L.) by genes associated with pre-har- vest sprouting resistancehttps://orcid.org/0000-0003-1502-303XГордейИ. С.GordejI. S.

Гордей Игорь Станиславович ‒ канд. биол. наук, доцент, заведующий лабораторией

ул. Академическая, 27, 220072, г. Минск

Igor S. Gordej ‒ Ph. D. (Biol.), Associate Professor, Head of Laboratory

27, Akademicheskaya Str., 220072, Minsk

I_Gordej777@mail.ruhttps://orcid.org/0000-0002-7243-6028ЛюсиковО. М.LyusikovO. M.

Люсиков Олег Михайлович ‒ науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Oleg M. Lyusikov ‒ Researcher

27, Akademicheskaya Str., 220072, Minsk

O.Lyusikov@igc.byМатиевскаяО. С.MatieuskayaO. S.

Матиевская Ольга Сергеевна – мл. науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Olga S. Matieuskaya – Junior Researcher

27, Akademicheskaya Str., 220072, Minsk

o.matieuskaya@igc.byhttps://orcid.org/0000-0002-4741-5823ШимкоВ. Е.ShimkoV. E.

Шимко Виктория Евгеньевна ‒ науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Victoria E. Shimko ‒ Researcher

27, Akademicheskaya Str., 220072, Minsk

shymko@mail.ruhttps://orcid.org/0000-0001-8674-4905МандрусоваВ. С.MandrusovaV. S.

Мандрусова Виктория Сергеевна ‒ мл. науч. cотрудник

ул. Академическая, 27, 220072, г. Минск

Victoria S. Mandrusova ‒ Junior Researcher

27, Akademicheskaya Str., 220072, Minsk

v.mandrusova@mail.ruСоколюкА. В.SokolyukА. V.

Соколюк Анна Владимировна – мл. науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Anna V. Sokolyuk – Junior Researcher

27, Akademicheskaya Str., 220072, Minsk

a.sokolyuk@igc.byhttps://orcid.org/0000-0001-6383-7703ВарфоломееваТ. Е.VarfalameyevaT. E.

Варфоломеева Татьяна Евгеньевна – мл. науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Tatyana E. Varfalameyeva – Junior Researche

27, Akademicheskaya Str., 220072, Minsk

t.varfalameyeva@igc.byИнститут генетики и цитологии Национальной академии наук БеларусиInstitute of Genetics and Cytology of the National Academy of Sciences of Belarus2026020220267113443Copyright © Гордей И.С., Люсиков О.М., Матиевская О.С., Шимко В.Е., Мандрусова В.С., Соколюк А.В., Варфоломеева Т.Е., 20262026Гордей И.С., Люсиков О.М., Матиевская О.С., Шимко В.Е., Мандрусова В.С., Соколюк А.В., Варфоломеева Т.Е.Gordej I.S., Lyusikov O.M., Matieuskaya O.S., Shimko V.E., Mandrusova V.S., Sokolyuk А.V., Varfalameyeva T.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/995

В настоящей работе проведено изучение современного генофонда озимой мягкой пшеницы (Triticum aestivum L.) с использованием ДНК-маркеров к генам TaMFT-3A, TaMKK3-A, TaMYB10 (R-1) и TaVp-B1, ассоциированным с устойчивостью к предуборочному прорастанию.

Полиморфизм анализируемого материала выявлен по генам TaMKK3-A и TaVp-B1. По гену TaMKK3-A 15 образцов (50 %) несли PhsS-аллель и 15 образцов (50 %) – PhsR-аллель. По гену TaVp-B1 6 образцов (20 %) характеризовались наличием PhsS-аллеля и 14 образцов (80 %) – PhsR-аллеля. По генам TaMFT-3A и TaMYB10 (R-1) селекционный материал был однороден. По SNP –222 гена TaMFT-3A все образцы имели PhsS-аллели. Также все образцы имели явно выраженный красный окрас зерновок, ассоциированный с наличием PhsR-аллелей генов TaMYB10 (R-1). В результате проведенного ДНК-маркирования современного генофонда селекционного материала озимой мягкой пшеницы по генам TaMFT-3A, TaMKK3-A и TaVp-B1, а также визуальной оценки окраса зерна (TaMYB10 (R-1)) выделено 14 потенциально наиболее устойчивых к предуборочному прорастанию образцов, которые имеют наиболее благоприятное сочетание аллелей по всем проанализированным генам.

Полученные данные могут быть использованы в практической селекции озимой мягкой пшеницы на устойчивость к предуборочному прорастанию.

This study investigated the modern gene pool of winter bread wheat (Triticum aestivum L.) based on the allelic state of the TaMFT-3A, TaMKK3-A, TaMYB10 (R-1) and TaVp-B1 genes associated with preharvest sprouting (PHS) resistance.

The polymorphism of analyzed material was detected by the TaMKK3-A and TaVp-B1 genes. By the TaMKK3-A gene, 15 accessions (50 %) carried the PhsS-allele, and 15 (50 %) had the PhsR-allele. By the TaVp-B1 gene, 6 accessions (20 %) exhibited the PhsS-allele, while 14 (80 %) possessed the PhsR-allele. The breeding material was monomorphic by the TaMFT-3A and TaMYB10 (R-1) genes. At the SNP –222 locus of TaMFT-3A, all accessions carried the PhsS-allele. Additionally, all samples displayed a distinct red grain color, associated with the presence of PhsR-alleles of the TaMYB10 (R-1) gene. As a result of the DNA marking of the modern wheat gene pool for the TaMFT-3A, TaMKK3-A, and TaVp-B1 genes, along with the visual grain color assessment (TaMYB10 (R-1)), 14 potentially most PHS-resistant accessions were identified, carrying the most favorable allelic combinations by all the genes analyzed.

The data obtained can be applied in the practical breeding of winter bread wheat for improved preharvest sprouting resistance.

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The authors declare that there are no conflicts of interest present.