vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2026-71-1-25-33vestib-994Research ArticleСтатьиАнтибактериальная активность экстрактов и отдельных групп метаболитов вегетативной массы Momordica charantia L. в отношении пищевых контаминантовAntibacterial activity of extracts and individual groups of metabolites of Momordica charantia L. vegetative mass against food contaminantsЛазаревичД. К.LazarevichD. K.

Лазаревич Дарья Константиновна – мл. науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Daria K. Lazarevich – Junior Researcher

27, Akademicheskaya Str., 220072, Minsk

darialazarevich743@gmail.comШевцовН. А.ShevtsovN. A.

Шевцов Николай Александрович – науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Nikolay A. Shevtsov – Researcher

27, Akademicheskaya Str., 220072, Minsk

sektor.metabo-lizma@mail.ruИвановО. А.IvanovO. A.

Иванов Олег Александрович – канд. биол. наук, веду- щий научный сотрудник

ул. Академическая, 27, 220072, г. Минск

Oleg A. Ivanov – Ph. D. (Biol.), Leading Researcher

27, Akademicheskaya Str., 220072, Minsk

protlife1984@gmail.comЦвиркоЕ. Д.TsvirkoE. D.

Цвирко Елена Дмитриевна – мл. науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Elena D. Tsvirko – Junior Researcher

27, Akademicheskaya Str., 220072, Minsk

elena-vasilev-skaya.foto@mail.ruИнститут экспериментальной ботаники имени В. Ф. Купревича Национальной академии наук БеларусиV. F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of Belarus2026020220267112533Copyright © Лазаревич Д.К., Шевцов Н.А., Иванов О.А., Цвирко Е.Д., 20262026Лазаревич Д.К., Шевцов Н.А., Иванов О.А., Цвирко Е.Д.Lazarevich D.K., Shevtsov N.A., Ivanov O.A., Tsvirko E.D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/994

Из различных видов мясной продукции, приобретенной в торговых сетях г. Минска и хранившейся при комнатной температуре в течение 2 суток, выделено и идентифицировано 8 штаммов бактерий – потенциальных контаминантов, в том числе впервые для Беларуси – грамотрицательная бактерия Aureimonas altamirensis. Штамм включен в белорусскую коллекцию непатогенных микроорганизмов Института микробиологии НАН Беларуси под номером БИМ B-2045. Продемонстрировано, что экстракты из вегетативной массы растения Momordica charantia L. сортов Индийский гранат, Крымская и Ажур способны подавлять развитие грамположительных штаммов бактерий (Enterococcus faecium БИМ В-1012, Microbacterium paraoxydans, Staphylococcus equorum) при концентрациях суммы веществ в экстракте не менее 4 мг/мл. Методом ТСХ-биоавтографии установлено, что антибактериальная активность экстрактов по отношению к штамму 13 S. equorum обеспечивается фенольными соединениями, гликозидами и агликонами флавонов с доминирующим вкладом полярных флавоновых агликонов. Продемонстрированы также межсортовые различия этих групп соединений в ТСХ-спектрах экстрактов, которые можно использовать при установлении сортовой принадлежности растений.

From various types of meat products purchased in Minsk retail chains and stored at room temperature for two days, 8 species of contaminant bacteria were isolated and identified, including, for the first time in Belarus, the Gram- negative bacterium Aureimonas altamirensis. The strain has been included in the Belarusian Collection of Non-Pathogenic Microorganisms at the Institute of Microbiology of the National Academy of Sciences of Belarus under the number BIM B-2045. It has been demonstrated that extracts from the vegetative mass of Momordica charantia L. varieties ‘Indian Pomegranate’,

‘Krymskaya’, and ‘Azhur’ are capable of inhibiting the growth of Gram-positive bacterial strains (Enterococcus faecium, Staphylococcus equorum, Microbacterium paraoxydans) at extract substance concentrations of at least 4 mg/ml. Using the TLC-bioautography method, it was established that the antibacterial activity of the extracts against the Staphylococcus equorum strain 13 is determined by phenolic compounds, glycosides, and flavone aglycones, with the dominant contribution provided by polar flavone aglycones. Inter-varietal differences in these groups of compounds were also demonstrated in the TLC spectra of the extracts, which can be used to determine the varietal affiliation of the plants.

Momordica charantiaмясные продуктыантибактериальные соединениябактерии-контаминантытонкослойная хроматографиябиоавтографияMomordica charantiameat productsantibacterial compoundscontaminant bacteriathin-layer chromatographybioautographyРабота выполнена при финансовой поддержке БРФФИ (проект № Б23М-009). Авторы выра- жают благодарность сотрудникам лаборатории «Кол- лекция микроорганизмов» Института микробиологии НАН Беларуси за предоставленные материалы для исследований.The work was carried out with the financial support of the BRFBR (project No. B23M-009). The authors express their gratitude to the staff of the labo- ratory Collection of Microorganisms of the Institute of Micro- biology of the National Academy of Sciences of Belarus for the research materials provided.ReferencesSilva, M. M. Food preservatives – An overview on applications and side effects / M. M. Silva, F. C. 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The authors declare that there are no conflicts of interest present.