vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2025-70-4-284-292vestib-985Research ArticleСтатьиАллельное разнообразие промотора гена лейкоантоцианидинредуктазы (PaLAR3) ели европейской в БеларусиAllelic diversity of the Norway spruce leucoanthocyanidin reductase gene (PaLAR3) promoter in BelarusНестюкА. М.NiastsiukA. M.

Нестюк Антонина Михайловна – науч. сотрудник.

ул. Академическая, 27, 220072, Минск

Antonina M. Niastsiuk – Researcher, V.F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of Belarus.

27, Akademicheskaya Str., 220072, Minsk

antonina.nestyuk95@gmail.comПантелеевС. В.PanteleevS. V.

Пантелеев Станислав Викторович – канд. биол. наук, доцент, заведующий лабораторией.

ул. Пролетарская, 71, 246050, Гомель

Stanislav V. Panteleev – Ph. D. (Biol.), Associate Professor, Head of the Laboratory, Forest Institute of the National Academy of Sciences of Belarus.

71, Proletarskaya Str., 246050, Gomel

stasikdesu@mail.ruПадутовА. В.PadutovA. V.

Падутов Александр Владимирович – науч. сотрудник.

ул. Пролетарская, 71, 246050, Гомель

Alexander V. Padutov – Researcher, Forest Institute of the National Academy of Sciences of Belarus.

71, Proletarskaya Str., 246050, Gomel

apadutov@yandex.byБарановО. Ю.BaranovO. Yu.

Баранов Олег Юрьевич – член-корреспондент, д-р биол. наук, профессор, академик-секретарь Отделения биологических наук НАН Беларуси.

пр-т Независимости, 66, 220072, Минск

Oleg Yu. Baranov – Corresponding Member, D. Sc. (Biol.), Professor, Academician-Secretary of the Department of Biological Sciences of the National Academy of Sciences of Belarus.

66, Nezavisimosti Ave., 220072, Minsk

betula-belarus@mail.ruИнститут экспериментальной ботаники имени В.Ф. Купревича Национальной академии наук БеларусиV.F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of BelarusИнститут леса Национальной академии наук БеларусиForest Research Institute of the National Academy of Sciences of BelarusОтделение биологических наук Национальной академии наук БеларусиDepartment of Biological Sciences of the National Academy of Sciences of Belarus202507112025704284292Copyright © Нестюк А.М., Пантелеев С.В., Падутов А.В., Баранов О.Ю., 20252025Нестюк А.М., Пантелеев С.В., Падутов А.В., Баранов О.Ю.Niastsiuk A.M., Panteleev S.V., Padutov A.V., Baranov O.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/985

В статье изложены результаты генетической оценки разнообразия аллельных вариантов промотора гена PaLAR3, детерминирующего отдельные этапы метаболизма флавоноидов ели европейской (Picea abies (L.) H. Karst.). Активность образования производных флаван-3,4-диола-(+)-катехина зависит от структуры промотора гена PaLAR3 и влияет на формирование устойчивости ели европейской к корневой гнили, вызываемой фитопатогенным грибом Heterobasidion parviporum Niemelä & Korhonen.

Экспериментальный материал для исследований был собран в 2017–2023 гг. с 950 деревьев ели европейской, произрастающих в различных регионах Беларуси как на лесосеменных плантациях I и II порядков, так и в лесных культурах.

В результате молекулярно-генетического анализа промотора гена PaLAR3 выявлено 8 размерных вариантов ампликонов, относящихся к 2 основным группам аллелей: А (А – 470 н. о., А1 – 488 н. о.) – восприимчивый к H. parviporum фенотип ели; В (В – 345 н. о., В1 – 342 н. о., С – 376 н. о., С1 – 394 н. о., D – 331 н. о. и D1 – 339 н. о.) – устойчивый фенотип. Проведенный анализ встречаемости аллельных вариантов среди всех изученных деревьев показал, что наибольшей представленностью характеризовалась восприимчивая группа аллелей А (65,40 %) и непосредственно аллель А (62,75 %). Группа B (34,60 %) включала в себя доминирующий аллельный вариант B (32,35 %) и его редкие разновидности с частотой встречаемости менее 1 %.

The article presents the results of a genetic evaluation of the diversity of allelic variants of the PaLAR3 gene promoter, which determines some stages of flavonoid metabolism in Norway spruce (Picea abies (L.) H. Karst.). The activity of production of flavan-3,4-diol-(+)-catechin derivatives depends on the structure of the PaLAR3 gene promoter and affects the formation of resistance of Norway spruce to spruce root rot, a condition induced by the phytopathogenic fungus Heterobasidion parviporum Niemelä & Korhonen.

The experimental material for the research was collected from 950 trees of European spruce during the period 2017–2023. These trees were growing in various regions of Belarus both in forest seed plantations of the 1st and 2nd generation and in forest crops.

A molecular genetic analysis of the PaLAR3 gene promoter revealed eight amplicon size variants belonging to two major allele groups: A (A – 470 bp, A1 – 488 bp) – susceptible phenotype of spruce to H. parviporum; B (B – 345 bp, B1 – 342 bp, C – 376 bp, C1 – 394 bp, D – 331 bp, and D1 – 339 bp) – resistant phenotype. The analysis of the occurrence of allelic variants among all the trees studied showed that the susceptible A allele group (65.40 %), and A allele (62.75 %) directly had the highest representation. Group B (34.60 %), included the dominant allele variant B (32.35 %) and its rare variants, with an incidence of less than 1 %.

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The authors declare that there are no conflicts of interest present.