vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2025-70-3-224-238vestib-979Research ArticleСтатьиХарактеристика генов, определяющих фитопатогенные, фитостимулирующие и антимикробные свойства бактерии Pseudomonas amygdali pv. lachrymans 8 – возбудителя угловатой пятнистости листьев огурцаCharacteristics of genes determining the phytopathogenic, phytostimulating and antimicrobial properties of Pseudomonas amygdali pv. lachrymans 8: a causative agent of angular leaf spot of cucumberМуратоваА. А.MuratovaA. A.

Муратова Анна Алексеевна – канд. биол. наук, ст. науч. сотрудник

ул. Академика Купревича, 2, 220084, г. Минск

Anna A. Muratova – Ph. D. (Biol.), Senior Researcher

2, Academician Kuprevich Str., 220084, Minsk

muratova@mbio.bas-net.byОхремчукА. Э.AkhremchukA. E.

Охремчук Артур Эдуардович – науч. сотрудник

ул. Академика Купревича, 2, 220084, г. Минск

Artur E. Akhremchuk – Researcher

2, Academician Kuprevich Str., 220084, Minsk

a.akhremchuk@mbio.bas-net.byВалентовичЛ. Н.ValentovichL. N.

Валентович Леонид Николаевич – канд. биол. наук, доцент, заведующий лабораторией

ул. Академика Купревича, 2, 220084, г. Минск

Leonid N. Valentovich – Ph. D. (Biol.), Associate Professor, Head of the Laboratory

2, Academician Kuprevich Str., 220084, Minsk

valentovich@mbio.bas-net.byИнститут микробиологии Национальной академии наук БеларусиInstitute of Microbiology of the National Academy of Sciences of Belarus202504082025703224238Copyright © Муратова А.А., Охремчук А.Э., Валентович Л.Н., 20252025Муратова А.А., Охремчук А.Э., Валентович Л.Н.Muratova A.A., Akhremchuk A.E., Valentovich L.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/979

В статье приведена информация о генетических детерминантах, продукты которых лежат как в основе патогенеза, так и в основе механизма стимулирования роста и развития растений бактериями Pseudomonas amygdali pv. lachrymans 8. В частности, в пределах генома штамма 8 выявлены гены, предположительно ответственные за проявление фитопатогенных свойств, кодирующие синтез липополисахаридов, компонентов систем секреции, ферментов, разрушающих клеточную стенку растений, жгутикового аппарата и системы хемотаксиса. Также у бактерий штамма 8 присутствуют генетические детерминанты, продукты которых способны положительно воздействовать на растения: гены биосинтеза 1-аминоциклопропан-1-карбоксилатдезаминазы, триптофана и антранилата, рибофлавина (витамина B2), а также гены, продукты которых определяют солюбилизацию неорганических фосфатов. В геноме штамма P. amygdali pv. lachrymans 8 обнаружено 12 локусов, ответственных за синтез вторичных метаболитов (нерибосомных пептидов, циановодорода, фурана, арилполиена, N-ацетилглутаминилглутаминамида, гомосеринлактона и пиовердина), которые способны предотвращать воздействие иных фитопатогенных микроорганизмов либо обеспечивать реакцию на стрессовые воздействия.

This article provides information on the genetic determinants, the products of which underlie both pathoge- nesis and the mechanism of stimulation of plant growth and development by Pseudomonas amygdali pv. lachrymans 8 bacteria. In particular, genes presumably responsible for the manifestation of phytopathogenic properties, encoding the synthesis of lipopolysaccharides, components of secretion systems, enzymes destroying the plant cell wall, flagellar apparatus, and chemotaxis system, were identified within the genome of strain 8. Furthermore, bacteria of strain 8 have genetic determinants whose products of which can positively affect plants. These determinants include genes for the biosynthesis of 1-aminocyclopropane-1-carboxylate deaminase, tryptophan and anthranilate, riboflavin (vitamin B2), as well as genes encoding products that determine the solubilization of inorganic phosphates. In the genome of the strain P. amygdali pv. lachrymans 8, 12 loci responsible for the synthesis of secondary metabolites (nonribosomal peptides, hydrogen cyanide, furan, arylpolyene, N-ace- tylglutaminyl glutaminamide, homoserine lactone and pyoverdine) were identified. These loci have the capacity to impede the effects of other phytopathogenic microorganisms or provide a response to stress effects.

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The authors declare that there are no conflicts of interest present.