References

vestib

Известия Национальной академии наук Беларуси. Серия биологических наук

Proceedings of the National Academy of Sciences of Belarus, Biological Series

1029-89402524-230X

The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"

10.29235/1029-8940-2025-70-2-135-145

vestib-969

Research Article

Статьи

Особенности структурно-функциональной организации генома бактерии Pseudomonas amygdali pv. lachrymans 8 – возбудителя угловатой пятнистости листьев огурца

Special aspects of structural and functional organization of the genome of Pseudomonas amygdali pv. lachrymans 8: a causative agent of angular leaf spot of cucumber

Муратова

А. А.

Muratova

A. A.

Муратова Анна Алексеевна – канд. биол. наук, ст. на- уч. сотрудник

ул. Академика Купревича, 2, 220084, г. Минск

Anna A. Muratova – Ph. D. (Biol.), Senior Researcher

2, Academician Kupre vich Str., 220084, Minsk

muratova@mbio.bas-net.by

Охремчук

А. Э.

Akhremchuk

A. E.

Охремчук Артур Эдуардович – науч. сотрудник

ул. Академика Купревича, 2, 220084, г. Минск

Artur E. Akhremchuk – Researcher

2, Academician Kupre vich Str., 220084, Minsk

a.akhremchuk@mbio.bas-net.by

Валентович

Л. Н.

Valentovich

L. N.

Валентович Леонид Николаевич – канд. биол. наук, доцент, заведующий лабораторией

ул. Академика Купревича, 2, 220084, г. Минск

Leonid N. Valentovich – Ph. D. (Biol.), Associate Profes- sor, Head of the Laboratory

2, Academician Kupre vich Str., 220084, Minsk

valentovich@mbio.basnet.by

Институт микробиологии Национальной академии наук БеларусиInstitute of Microbiology of the National Academy of Sciences of Belarus

2025

08052025

702135145

2025

Муратова А.А., Охремчук А.Э., Валентович Л.Н.

Muratova A.A., Akhremchuk A.E., Valentovich L.N.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vestibio.belnauka.by/jour/article/view/969

В статье отражены результаты секвенирования, молекулярно-генетического и сравнительного анализа генома фитопатогенной бактерии Pseudomonas amygdali pv. lachrymans 8. Собранная геномная последовательность депонирована в базу данных GenBank Национального центра биотехнологической информации (НЦБИ) США (номера для доступа: CP075686–CP075690). В результате расчета средней нуклеотидной идентичности определено, что последовательность генома штамма 8 имеет сходство 99,87 % и 99,79 % с последовательностями геномов бактерий P. amygdali pv. lachrymans 814/98 и P. amygdali pv. lachrymans M301315 соответственно. Установлено, что геном штамма 8 представлен кольцевой хромосомой размером 6 054 652 п. н. с содержанием ГЦ-пар 58,11 % и четырьмя кольцевыми плазмидами: pPAL8-01 (77 748 п. н., содержание ГЦ-пар 56 %), pPAL8-02 (72 398 п. н., содержание ГЦ-пар 55 %), pPAL8-03 (49 000 п. н., содержание ГЦ-пар 54 %) и pPAL8-04 (9600 п. н., содержание ГЦ-пар 55 %). Высказано предположение, что путь распространения изучаемого фитопатогена осуществлялся либо параллельно из Нидерландов в США и Беларусь, либо сначала из Нидерландов на территорию США, а затем в Беларусь. Осуществлено сравнение нуклеотидной последовательности штамма 8 с нуклеотидными последовательностями бактерий P. amygdali pv. lachrymans M301315, NM002 и YM7902, в результате чего выявлены значительные генетические перестройки и определена локализация уникальной области размером 19 773 п. н.

This article presents the results of the sequencing, molecular genetic and comparative analysis of the genome of the phytopathogenic bacterium Pseudomonas amygdali pv. lachrymans 8. The assembled genome sequence has been deposited in the GenBank database of the US National Center for Biotechnology Information (accession numbers: CP075686–CP075690). The calculation of the average nucleotide identity revealed that the genome sequence of strain 8 exhibits 99.87 and 99.79 % similarity with the genome sequences of bacteria P. amygdali pv. lachrymans 814/98 and P. amygdali pv. lachrymans M301315, respectively. The genome of strain 8 was found to be represented by a circular chromosome of 6,054,652 bp with a GC-pair content of 58.11 % and four circular plasmids: pPAL8-01 (77,748 bp, GC-pair content of 56 %), pPAL8-02 (72,398 bp, GC-pair content of 55 %), pPAL8-03 (49,000 bp, GC-pair content of 54 %) and pPAL8-04 (9,600 bp, GC-pair content of 55 %). It is suggested that the route of dissemination of the studied phytopathogen was either parallel from the Netherlands to the USA and Belarus, or first from the Netherlands to the USA and then to Belarus. A comparison of the nucleotide sequence of the strain 8 with the nucleotide sequences of the bacteria P. amygdali pv. lachrymans M301315, NM002 and YM7902 revealed significant genetic rearrangements and determined the localization of a unique region of 19,773 bp.

псевдомонадыфитопатогенполногеномное секвенированиеанализ геномагенетические перестройки

pseudomonadsphytopathogenwhole genome sequencinggenome analysisgenetic rearrangements

Исследования выполнены при финансовой поддержке государственной программы научных исследований «Биотехнологии» (задание 3.03) и Белорусского республиканского фонда фундаментальных исследований (договор № Б23М-076).

The research was carried out with the financial support of the State Program of Scientific Research “Biotechnologies” (task 3.03) and the Belarusian Republican Foundation for Basic Research (contract No. B23M-076).

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The authors declare that there are no conflicts of interest present.