vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2025-70-2-95-107vestib-965Research ArticleСтатьиАктивность фотосинтетических мембран в инфицированных Fusarium oxysporum растениях огурца (Сucumis sativus L.) при разной доле синего светодиодного освещенияActivity of photosynthetic membranes in Fusarium oxysporum-infected cucumber (Сucumis sativus L.) plants under different proportions of blue LED illuminationДоманскаяИ. Н.DomanskayaI. N.

Доманская Ирина Николаевна – канд. биол. наук, доцент, ст. науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Irina N. Domanskaya – Ph. D. (Biol.), Аssociate Рrofessor, Senior Researcher

27, Akademicheskaya Str., 220072, Minsk

domanin07@mail.ruАртемчукЯ. Н.ArtemchukYa. N.

Артемчук Яна Николаевна – мл. науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Yana N. Artemchuk – Junior Researcher

27, Akademicheskaya Str., 220072, Minsk

yanaart222@gmail.comГордиенкоС. С.GordienkoS. S.

Гордиенко Софья Сергеевна – мл. науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Sofya S. Gordienko – Junior Researcher

27, Akademicheskaya Str., 220072, Minsk

gordienko.sonya@inbox.ruМолчанО. В.MolchanO. V.

Молчан Ольга Викторовна – канд. биол. наук, доцент, заведующий лабораторией

ул. Академическая, 27, 220072, г. Минск

Olga V. Molchan – Ph. D. (Biol.), Аssociate Рrofessor, Head of the Laboratory

27, Akademicheskaya Str., 220072, Minsk

olga_molchan@mail.ruКабашниковаЛ. Ф.KabashnikovaL. F.

Кабашникова Людмила Федоровна – член-корреспондент, д-р биол. наук, доцент, заведующий лабораторией

ул. Академическая, 27, 220072, г. Минск

Lyudmila F. Kabashnikova – Corresponding Member, D. Sc. (Biol.), Assistant Professor, Head of the Laboratory

27, Akademicheskaya Str., 220072, Minsk

kabashnikova@ibp.org.byИнститут биофизики и клеточной инженерии Национальной академии наук БеларусиInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of BelarusИнститут экспериментальной ботаники имени В. Ф. Купревича Национальной академии наук БеларусиInstitute of Experimental Botany named after V. F. Kuprevich of the National Academy of Sciences of Belarus20250805202570295107Copyright © Доманская И.Н., Артемчук Я.Н., Гордиенко С.С., Молчан О.В., Кабашникова Л.Ф., 20252025Доманская И.Н., Артемчук Я.Н., Гордиенко С.С., Молчан О.В., Кабашникова Л.Ф.Domanskaya I.N., Artemchuk Y.N., Gordienko S.S., Molchan O.V., Kabashnikova L.F.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/965

Исследовано влияние LED-освещения с разной долей (20 и 60 %) синего света (СС) и разной длительности на функционирование фотосистем (ФС) в здоровых и инфицированных грибом Fusarium oxysporum (Fus. oxy.) листьях огурца. Длительный световой режим (25 дней) с высокой долей СС, 60 % и последующее заражение Fus. oxу. подавляло функциональную активность ФСII относительно белого света (БС), что выразилось в значительном снижении максимальной флуоресценции временно закрытых реакционных центров ФСII (Fm), эффективного квантового выхода фотохимических реакций (Fv/Fm), максимальной квантовой эффективности ФСII (Y(II)), а также в изменении характера перераспределения поглощенной световой энергии. Это привело к снижению интенсивности фотохимической конверсии (qP), количества открытых реакционных центров ФСII (qL) и скорости транспорта электронов через ФСII (ETR(II)). При 7-дневной экспозиции растений в разных световых условиях основные изменения параметров ФСII наблюдались только в инфицированных листьях, сформированных в режиме СС, 60 %.

Выращивание растений на БС и СС, 20 % лишь незначительно изменяло вклад потока электронов на донорной и акцепторной сторонах ФСI в инфицированных листьях огурца, не затронув уровень окисленности Р700 и квантовый выход фотохимических реакций. Заражение растений, выращенных длительное время на СС, 60 %, вызывало 10-кратное снижение квантового выхода фотохимических реакций ФСI и существенное повышение нефотохимической диссипации энергии на донорной и акцепторной сторонах ФСI. Стрессовое воздействие патогена усиливало также подавляющий эффект СС, 60 % при короткой экспозиции, что нашло свое отражение в существенном уменьшении таких показателей, как квантовый выход ФСI (Y(I)) и эффективность переноса электронов в электрон-транспортной цепи ФСI (ETR(I)).

Полученные результаты могут быть использованы как методическая основа для создания энергосберегающих светодиодных светильников, оптимизированных для выращивания растений огурца в закрытом грунте, а также для контроля степени заражения растений на ранних стадиях фузариоза.

The effect of LED illumination with different proportions of blue light (BL, 20 and 60 %) and different durations on the functionality of photosystems (PS) in healthy and infected with the fungus Fusarium oxysporum (Fus. oxy.) cucumber leaves was studied. Long-term exposure (25 days) to a light regime with a high share of BL, 60 % and subsequent infection with Fus. oxy., suppressed the functional activity of PSII relative to white light (WL), which was reflected in a significant decrease in the maximum fluorescence of temporarily closed PSII reaction centers (Fm), the effective quantum yield of photochemical reactions (Fv/Fm), the maximum quantum efficiency of PSII (Y(II)), as well as in changes to the nature of the redistribution of absorbed light energy. This resulted in a decline in the intensity of photochemical conversion (qP), the number of open PSII reaction centers (qL) and the rate of electron transport through PSII (ETR(II)). During a 7-day exposure of plants to different light conditions, the main changes in PSII parameters were observed only in infected leaves formed in the BL, 60 %.

Growing on WL and BL, 20 % only slightly changed the contribution of the electron flow on the donor and acceptor sides of PSI in infected cucumber leaves, without affecting the level of P700 oxidation and the quantum yield of photochemical reactions. Infection of plants grown for a long time on BL, 60 % caused a 10-fold decrease in the quantum yield of photochemical reactions of PSI and a significant increase in non-photochemical energy dissipation on the donor and acceptor sides of PSI. The stress effect of the pathogen also enhanced the suppressive effect of BL, 60 % at short exposure, which was reflected in a significant decrease of such parameters as the quantum yield of PSI (Y(I)) and the efficiency of electron transfer in the electron transport chain of PSI (ETR(I)).

The results obtained can be used as a methodological basis for the development of energy-saving LED light sources optimized for growing cucumber plants in closed soil, as well as for monitoring the degree of infection of plants in the early stages of Fusarium infection.

огурецLED-освещениесиний светфотосистема Iфотосистема IICucumis sativus L.Fusarium oxysporumсucumberLED lightingblue lightphotosystem Iphotosystem IICucumis sativus L.Fusarium oxysporumРабота выполнена в рамках ГПНИ «Биотехнологии-2» на 2021–2025 гг. подпрограммы 1 «Молекулярные и клеточные биотехнологии-2» «Изучить влияние синего LED-освещения на молекулярно-мембранные механизмы взаимодействия грибных патогенов рода Fusarium с растительными клетками».The work was carried out within the framework of the State Program of Scientific Research “Biotechnology-2” for 2021–2025, Subprogram 1 “Molecular and Cellular Biotechnology-2” “To study the effect of blue LED illumination on the molecular membrane mechanisms of interaction of fungal pathogens of the genus Fusarium with plant cells”.ReferencesCasal, J. J. Phytochromes, cryptochromes, phototropin: photoreceptor interactions in plants / J. J. 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The authors declare that there are no conflicts of interest present.