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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vestib</journal-id><journal-title-group><journal-title xml:lang="ru">Известия Национальной  академии наук Беларуси. Серия биологических наук</journal-title><trans-title-group xml:lang="en"><trans-title>Proceedings of the National Academy of Sciences of Belarus, Biological Series</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1029-8940</issn><issn pub-type="epub">2524-230X</issn><publisher><publisher-name>The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.29235/1029-8940-2025-70-1-69-79</article-id><article-id custom-type="elpub" pub-id-type="custom">vestib-956</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group></article-categories><title-group><article-title>Установление породного состава свиней с использованием технологии KASP</article-title><trans-title-group xml:lang="en"><trans-title>Establishing the breed composition of pigs using KASP technology</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кипен</surname><given-names>В. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Kipen′</surname><given-names>V. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Кипень Вячеслав Николаевич – канд. биолог. наук, доцент, вед. науч. сотрудник</p><p>ул. Академическая, 27, 220072, г. Минск</p></bio><bio xml:lang="en"><p>Vуacheslav N. Kipen′ – Ph. D. (Biol.), Associate Professor, Leading Researcher</p><p>27, Academicheskaya Str., 220072, Minsk</p></bio><email xlink:type="simple">v.kipen@igc.by</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Снытков</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Snytkov</surname><given-names>E. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Снытков Евгений Владимирович – науч. сотрудник</p><p>ул. Академическая, 27, 220072, г. Минск</p></bio><bio xml:lang="en"><p>Evgenij V. Snytkov – Researcher</p><p>27, Academicheskaya Str., 220072, Minsk</p></bio><email xlink:type="simple">evsnytkov@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Михайлова</surname><given-names>М. Е.</given-names></name><name name-style="western" xml:lang="en"><surname>Mikhailova</surname><given-names>M. E.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Михайлова Мария Егоровна – канд. биолог. наук, доцент, заведующий лабораторией</p><p>ул. Академическая, 27, 220072, г. Минск</p></bio><bio xml:lang="en"><p>Mariya E. Mikhailova – Ph. D. (Biol.), Associate Professor, Head of Laboratory</p><p>27, Academicheskaya Str., 220072, Minsk</p></bio><email xlink:type="simple">M.Mikhailova@igc.by</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Институт генетики и цитологии НАН Беларуси</institution></aff><aff xml:lang="en"><institution>Institute of Genetics and Cytology of the National Academy of Sciences of Belarus</institution></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>28</day><month>01</month><year>2025</year></pub-date><volume>70</volume><issue>1</issue><fpage>69</fpage><lpage>79</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Кипен В.Н., Снытков Е.В., Михайлова М.Е., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Кипен В.Н., Снытков Е.В., Михайлова М.Е.</copyright-holder><copyright-holder xml:lang="en">Kipen′ V.N., Snytkov E.V., Mikhailova M.E.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vestibio.belnauka.by/jour/article/view/956">https://vestibio.belnauka.by/jour/article/view/956</self-uri><abstract><p>Установлен высокий дифференцирующий потенциал семи полиморфных вариантов для различения свиней пород йоркшир, белорусская крупная белая и белорусская мясная, разводимых в Беларуси. Для свиней породы йоркшир предложена модель, включающая три однонуклеотидных полиморфизма – Chr.6:g.121005974A&gt;G, Chr.17:g.15827832G&gt;T, Chr.10:g.30081932A&gt;G; для свиней породы белорусская крупная белая – Chr.6:g.121005974A&gt;G, Chr.3:g.118879246C&gt;G, Chr.7:g.52269732A&gt;G; для свиней породы белорусская мясная – Chr.8:g.47482649G&gt;T, Chr.9:g.48882095A&gt;G, Chr.10:g.30081932A&gt;G. Модели характеризуются высокими значениями точности, специфичности и чувствительности. Подход к дифференциации пород свиней основан на технологии конкурентной аллельспецифической ПЦР.</p></abstract><trans-abstract xml:lang="en"><p>A high differentiating potential of seven polymorphic variants has been identified to distinguish between Yorkshire, Belarusian Large White and Belarusian Meat pig breeds bred in Belarus. For the Yorkshire pig breed, a model is proposed including three single nucleotide polymorphisms – Chr.6:g.121005974A&gt;G, Chr.17:g.15827832G&gt;T, Chr.10:g.30081932A&gt;G; for the Belarusian Large White pig breed – Chr.6:g.121005974A&gt;G, Chr.3:g.118879246C&gt;G, Chr.7:g.52269732A&gt;G; for the Belarusian Meat pig breed – Chr.8:g.47482649G&gt;T, Chr.9:g.48882095A&gt;G, Chr.10:g.30081932A&gt;G. The models demonstrate high accuracy, specificity, and sensitivity values. The approach to differentiating pig breeds is based on the technology of сompetitive allele-specific PCR.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>Sus scrofa domesticus</kwd><kwd>йоркшир</kwd><kwd>белорусская крупная белая</kwd><kwd>белорусская мясная</kwd><kwd>однонуклеотидный полиморфизм</kwd><kwd>дифференциация</kwd><kwd>конкурентная аллель-специфическая ПЦР</kwd><kwd>генотипирование in silico</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Sus scrofa domesticus</kwd><kwd>Yorkshire</kwd><kwd>Belarusian Large White</kwd><kwd>Belarusian Meat</kwd><kwd>single nucleotide polymorphism</kwd><kwd>differentiation</kwd><kwd>сompetitive allele-specific PCR (KASP)</kwd><kwd>in silico genotyping</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Молекулярно-генетические исследования выполнены в рамках ГПНИ «Биотехнологии-2», подпрограмма «Геномика, эпигеномика, биоинформатика» на 2021–2025 гг., задание «Разработка системы генетического анализа для определения чистопородности свиней на основе изучения SNP-локусов» (2021–2023 гг., рег. № 20210339). Программное обеспечение GENIS разработано в рамках НИР «Биоинформатический подход к анализу данных полногеномного секвенирования для поиска однонуклеотидных замен, способных дифференцировать близкородственные биологические виды» (БРФФИ, 2023–2025 гг., Б23-060, рег. № 20231076).</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Dekkers, J. C. M. Marker-assisted selection for commercial crossbred performance / J. C. M. Dekkers // Journal of Animal Science. – 2007. – Vol. 85, N 9. – P. 2104–2114. https://doi.org/10.2527/jas.2006-683</mixed-citation><mixed-citation xml:lang="en">Dekkers J. C. M. Marker-assisted selection for commercial crossbred performance. 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