vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2024-69-3-249-253vestib-929Research ArticleСтатьиПолучение комплексов 3′,5′-циклического диаденозинмонофосфата с Mg,Al-слоистым двойным гидроксидомProduction of 3′,5′-cyclic diadenosine monophosphate complex with Mg,Al-layered double hydroxideВинтерМ. А.VinterM. A.

Винтер Маргарита Андреевна – аспирант.

ул. Купревича, 2, 220141, Минск

Marharyta A. Vinter – Postgraduate Student, Institute of Microbiology of the National Academy of Sciences of Belarus.

2, Kuprevich Str., 220141, Minsk

rita.vinter.abc@gmail.comЩеколоваА. С.ShchokolovaA. S.

Щеколова Анастасия Сергеевна – канд. биол. наук, ст. науч. сотрудник.

ул. Купревича, 2, 220141, Минск

Anastasia S. Shchokolova – Ph. D. (Biol.), Senior Researcher, Institute of Microbiology of the National Academy of Sciences of Belarus.

2, Kuprevich Str., 220141, Minsk

nastindeathnote@gmail.comКазловскийИ. С.KazlouskiI. S.

Казловский Илья Сергеевич – канд. биол. наук, вед. науч. сотрудник.

ул. Купревича, 2, 220141, Минск

Illia S. Kazlouski – Ph. D. (Biol.), Leading Researcher, Institute of Microbiology of the National Academy of Sciences of Belarus.

2, Kuprevich Str., 220141, Minsk

kazlouski.illia@gmail.comhttps://orcid.org/0000-0003-2401-2586ЗинченкоА. И.ZinchenkoA. I.

Зинченко Анатолий Иванович – член-корреспондент, д-р биол. наук, профессор, заведующий лабораторией.

ул. Купревича, 2, 220141, Минск

Anatoly I. Zinchenko – Corresponding Member, D. Sc. (Biol.), Professor, Head of the Laboratory, Institute of Microbiology of the National Academy of Sciences of Belarus.

2, Kuprevich Str., 220141, Minsk

zinch@mbio.bas-net.byИнститут микробиологии НАН БеларусиInstitute of Microbiology of the National Academy of Sciences of Belarus202431072024693249253Copyright © Винтер М.А., Щеколова А.С., Казловский И.С., Зинченко А.И., 20242024Винтер М.А., Щеколова А.С., Казловский И.С., Зинченко А.И.Vinter M.A., Shchokolova A.S., Kazlouski I.S., Zinchenko A.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/929

Показана возможность использования Mg,Al-слоистого двойного гидроксида (СДГ) для получения наночастиц, включающих молекулы фармацевтически важного 3′,5′-циклического диаденозинмонофосфата (циклоди-АМФ). Подобраны экспериментальные условия для получения комплексов СДГ с циклоди-АМФ (размером порядка 300 нм), емкость которых в отношении циклоди-АМФ достигает 60 мас.%. Установлено, что при рН среды 4,5 циклоди-АМФ высвобождается из его комплекса с СДГ более активно, чем при рН 7,4. Полученные результаты свидетельствуют о возможности применения изученных нанокомплексов для пролонгированной доставки циклоди-АМФ в иммунные клетки-мишени.

The possibility of using Mg,Al-layered double hydroxide (LDH) for production of nanoparticles comprising molecules of pharmaceutically valuable 3′,5′-cyclic diadenosine monophosphate (cyclic di-AMP) was demonstrated. Experimental conditions were optimized to produce LDH complexes with cyclic di-AMP (average size of 300 nm), which loading capacity in regard to the target compound reached 60 mass %. The fact of cyclic di-AMP release from LDH complex in pH-dependent mode (proceeding slightly slower at pH 7.4 than at pH 4.5) was stated. The obtained results testify in favor of applying the studied nanocomplexes for delayed delivery of cyclic di-AMP to the target immune cells.

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The authors declare that there are no conflicts of interest present.