vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2024-69-2-143-152vestib-918Research ArticleСтатьиВлияние некоторых абиотических факторов на профили экспрессии генов, кодирующих стресс-ассоциированные белки яблониInfluence of some abiotic factors on the apple stressassociated protein gene expression profileshttps://orcid.org/0000-0002-1929-4315КузмицкаяП. В.KuzmitskayaP. V.

Кузмицкая Полина Викторовна – канд. биол. наук, ст. науч. сотрудник

ул. Ф. Скорины, 34, 220141, г. Минск

Polina V. Kuzmitskaya – Ph. D. (Biol.), Senior Researcher

34, F. Skoriny Str., 220141, Minsk

p.kuzmitskaya@igc.byКоролеваЕ. С.KaralevaK. S.

Королева Екатерина Сергеевна – мл. науч. сотрудник

ул. Ф. Скорины, 34, 220141, г. Минск

Katsiaryna S. Karaleva – Junior Researcher

34, F. Skoriny Str., 220141, Minsk

e.koroleva@igc.byУрбановичО. Ю.UrbanovichO. Yu.

Урбанович Оксана Юрьевна –д-р биол. наук, доцент, заведующий лабораторией

ул. Ф. Скорины, 34, 220141, г. Минск

Oksana Yu. Urbanovich – D. Sc. (Biol.), Associate Professor, Head of the Laboratory

34, F. Skoriny Str., 220141, Minsk

o.urbanovich@igc.byИнститут генетики и цитологии НАН БеларусиInstitute of Genetics and Cytology of the National Academy of Sciences of Belarus202428042024692143152Copyright © Кузмицкая П.В., Королева Е.С., Урбанович О.Ю., 20242024Кузмицкая П.В., Королева Е.С., Урбанович О.Ю.Kuzmitskaya P.V., Karaleva K.S., Urbanovich O.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/918

Стресс-ассоциированные белки (SAP, stress-associated proteins) принимают участие в формировании у растений ответа на действие неблагоприятных биотических и абиотических факторов. В представленной работе с помощью количественной ПЦР (qPCR) проведена оценка профилей экспрессии 14 генов яблони, кодирующих SAP (MdSAP), при воздействии низких и высоких температур, а также засоления. Показано, что наиболее значимое изменение уровней экспрессии наблюдалось при воздействии повышенной температуры (гены MdSAP11, MdSAP2, MdSAP3), в условиях пониженной температуры (MdSAP1, MdSAP2, MdSAP4, MdSAP6) и при воздействии раствора соли (MdSAP1, MdSAP8, MdSAP11). При этом наблюдалась тенденция к повышению экспрессии генов ко 2-му и/или 4-му часу воздействия с последующим ее снижением к 24-му часу. Анализ корреляции уровней экспрессии генов MdSAP в стрессовых условиях показал, что для них характерны как положительные, так и отрицательные линейные связи.

Полученные результаты позволяют уточнить роль отдельных генов, кодирующих SAP, в формировании стрессового ответа яблони на воздействие низких и высоких температур, а также засоления.

In plants, stress-associated proteins (SAP) play a crucial role in responding to adverse biotic and abiotic factors. In this study, we evaluated the expression profiles of 14 apple SAP genes (MdSAP) under the influence of low and high temperatures, as well as salinity, using the quantitative polymerase chain reaction (qPCR). The results showed that the most significant changes in expression levels were observed in the genes MdSAP11, MdSAP2, and MdSAP3 under high temperature conditions, MdSAP1, MdSAP2, MdSAP4, and MdSAP6 under low temperature conditions, and MdSAP1, MdSAP8, and MdSAP11 under salt stress. Furthermore, there was a tendency for gene expression to increase at 2 and/or 4 hours of exposure followed by a decrease at 24 hours. The analysis of the correlation between the expression levels of MdSAP genes revealed both positive and negative linear relationships.

These findings will help elucidate the specific roles of individual SAP genes in shaping the apple stress response to low and high temperatures, as well as salinity.

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The authors declare that there are no conflicts of interest present.