vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2024-69-1-68-78vestib-911Research ArticleСтатьиАнализ проблемы молекулярной идентификации дикого (wtCT), бесплазмидного (р-СТ) и шведского (SE-nvCT) вариантов Chlamydia trachomatis в БеларусиAnalysis of the problem of molecular identification of wild (wtCT), plasmidless (p-CT) and Swedish (SE-nvCT) variants of Chlamydia trachomatis in Belarushttps://orcid.org/0000-0002-7963-0026РубаникЛ. В.RubanikL. V.

Рубаник Людмила Владимировна ‒ канд. биол. наук, доцент, заведующий лабораторией

ул. Филимонова, 23, 220114, г. Минск

Lуudmila V. Rubanik ‒ Ph. D. (Biol.), Associate Professor, Head of the Laboratory

23, Filimonov Str., 220114, Minsk

rubaniklv@tut.byhttps://orcid.org/0000-0003-1083-6680ПолещукН. Н.PoleshchukN. N.

Полещук Николай Николаевич ‒ д-р мед. наук, профессор, гл. науч. cотрудник

ул. Филимонова, 23, 220114, г. Минск

Nikolay N. Poleshchuk ‒ D. Sc. (Med.), Professor, Chief Researcher

23, Filimonov Str., 220114, Minsk

pnn@belriem.byРеспубликанский научно-практический центр эпидемиологии и микробиологииRepublican Scientific and Practical Center of Epidemiology and Microbiology2024080220246916878Copyright © Рубаник Л.В., Полещук Н.Н., 20242024Рубаник Л.В., Полещук Н.Н.Rubanik L.V., Poleshchuk N.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/911

К настоящему времени известно, что популяция Chlamydia trachomatis генетически гетерогенна. Наряду с первоначально описанным диким типом (wtCT) в мире обнаружены мутантные варианты (mtCT): бесплазмидный (р-СТ), шведский (SE-nvCT), мексиканский (MX-nvCT) и финский (FI-nvCT), обладающие неодинаковой вирулентностью и тропностью к различным органам и тканям. Данные варианты могут ускользать от ПЦРдиагностики ввиду отсутствия целевых мишеней или изменений в них, что делает неэффективным использование ряда диагностических тест-систем для молекулярно-биологической детекции патогена.

Проанализированы изоляты C. trachomatis, собранные на территории Республики Беларусь в период с 2013 по 2022 г. от лиц репродуктивного возраста с воспалительными заболеваниями урогенитального тракта. Установлено, что доминирующим геновариантом возбудителя (примерно в 93 % случаев) является дикий тип wtCT. Мутантные штаммы, составляющие около 7 % популяции патогена, представлены p-CT и SE-nvCT геновариантами. Случаев выявления MX-nvCT и FI-nvCT геновариантов в анализируемой выборке изолятов C. trachomatis не отмечено.

Необходимы дальнейшая оптимизация тактики молекулярно-биологической идентификации различных геновариантов C. trachomatis для эффективного обнаружения возбудителя и изучение патогенеза хламидийной урогенитальной инфекции.

To date, it is known that the population of Chlamydia trachomatis is genetically heterogeneous. Along with the originally described wild type (wtCT), mutant variants (mtCT) have been found in the world: plasmidless (p-CT), Swedish (SE-nvCT), Mexican (MX-nvCT), Finnish (FI-nvCT), with different virulence and tropicity to various organs and tissues. These variants may escape PCR diagnostics due to the absence of targets or the occurrence of changes in them, which makes it ineffective to use a number of diagnostic test systems for pathogen detection.

Isolates of C. trachomatis collected on the territory of the Republic of Belarus during the period 2013–2022 in reproductive age persons with inflammatory urogenital tract diseases were analyzed. It was found that the dominant pathogen genovariant is the wild type wtCT ‒, approximately 93 %. Mutant strains that make up about 7 % of the pathogen population are represented by p-CT and SE-nvCT genovariants. There were no cases of identification of MX-nvCT and FI-nvCT genovariants in the analyzed sample of C. trachomatis isolates.

It is necessary to further optimize the tactics of molecular biological identification of various C. trachomatis genovariants for effective microorganism detection and study of the chlamydial urogenital infection pathogenesis.

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The authors declare that there are no conflicts of interest present.