vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2024-69-1-15-24vestib-906Research ArticleСтатьиФизиолого-биохимические особенности реализации адаптивного потенциала клонально микроразмноженных растений картофеля при их обработке иммуностимуляторамиPhysiological and biochemical features of implementation of the adaptive potential of clonally micropropagated potato plants using immunostimulantsБалюкН. В.BaliukN. V.

Балюк Наталья Валерьевна – канд. биол. наук

ул. Академическая, 27, 2200072, г. Минск

Natallia V. Baliuk – Ph. D. (Biol.)

27, Akademicheskaya Str., 2200072, Minsk

balyuck.natalya@yandex.ruhttps://orcid.org/0000-0002-1067-4936ЛаманН. А.LamanN. A.

Ламан Николай Афанасьевич – академик, д-р биол. наук, профессор, заведующий лабораторией

ул. Академическая, 27, 2200072, г. Минск

Nikolai A. Laman – Academiсian, D. Sc. (Biol.), Professor, Head of the Laboratory

27, Akademicheskaya Str., 2200072, Minsk

laman.nikolai@gmail.comhttps://orcid.org/0000-0001-6395-0757КалацкаяЖ. Н.KalatskajaJ. N.

Калацкая Жанна Николаевна – канд. биол. наук, вед. науч. сотрудник

ул. Академическая, 27, 2200072, г. Минск

Joanna N. Kalatskaja – Ph. D. (Biol.), Leading Researcher

27, Akademicheskaya Str., 2200072, Minsk

kalatskayaj@mail.ruИнститут экспериментальной ботаники имени В. Ф. Купревича НАН БеларусиV. F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of Belarus2024080220246911524Copyright © Балюк Н.В., Ламан Н.А., Калацкая Ж.Н., 20242024Балюк Н.В., Ламан Н.А., Калацкая Ж.Н.Baliuk N.V., Laman N.A., Kalatskaja J.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/906

Изучено влияние эпибрассинолида в сочетании с салициловой кислотой и метилжасмонатом на формирование комплексной устойчивости клонально микроразмноженных растений картофеля сорта Бриз к вирусу Y картофеля и недостатку влаги, функционирование про-/антиоксидантной системы, а также на физиологическое состояние, продуктивность и качество полученных мини-клубней. Выявлено, что антивирусная устойчивость в условиях комбинированного стресса формируется только в присутствии эпибрассинолида, который запускает серию защитных механизмов за счет накопления перекиси водорода, фенольных соединений и возрастания активности пероксидаз, в результате чего сохраняется продуктивность и улучшается качество получаемой продукции. Применение трехкомпонентной смеси позволяет получить наибольшие массу и количество мини-клубней картофеля, при этом ее защитное действие против вирусной инфекции не проявляется.

The effect of epibrassinolide in combination with salicylic acid and methyl jasmonate on the formation of complex resistance of clonally micropropagated potato plants of the Briz variety to the potato virus Y and to moisture lack, their physiological state, the functioning of the pro-/antioxidant system, as well as on the productivity and quality of the obtained mini-tubers was studied. It was revealed that antiviral resistance under combined stress conditions is formed only in the presence of epibrassinolide that triggers a series of protective mechanisms through accumulation of hydrogen peroxide, phenolic compounds and an increase in peroxidase activity, while maintaining productivity and improving the quality of the products obtained. The use of a three-component mixture is accompanied by the largest mass and a number of potato minitubers, while its protective effect against a viral infection is not manifested.

Solanum tuberosumнедостаток влагивирус Y картофеляэпибрассинолидсалициловая кислотаметилжасмонатперекись водородаантиоксидантные ферментыпролинфенольные соединенияSolanum tuberosummoisture deficiencypotato virus Yepibrassinolidesalicylic acidmethyl jasmonatehydrogen peroxideantioxidant enzymesprolinephenolic compoundsРабота выполнена при финансовой поддержке Белорусского республиканского фонда фундаментальных исследований (проект № Б22М-037).This work was financially supported by the Belarusian Republican Foundation for Fundamental Research (project no. B22M-037).ReferencesCombined biotic and abiotic stress resistance in tomato / S. Kissoudis [et al.] // Euphytica. – 2015. – Vol. 202. – P. 317– 332. https://doi.org/10.1007/s10681-015-1363-xKissoudis C., Chowdhury R., van Heusden S., van de Wiel C., Finkers R., Visser R. G., Bai Y., van der Linden G. Combined biotic and abiotic stress resistance in tomato. Euphytica, 2015, vol. 202, pp. 317–332. https://doi.org/10.1007/s10681- 015-1363-xEnvironment and host genotype determine the outcome of a plant-virus interaction: from antagonism to mutualism / J. Hily [et al.] // New Phytologist. – 2016. – Vol. 209, N 2. – P. 812–822. https://doi.org/10.1111/nph.13631Hily J.-M., Poulicard N., Mora M.-Á., Pagán I., García‐Arenal F. Environment and host genotype determine the outcome of a plant-virus interaction: from antagonism to mutualism. New Phytologist, 2016, vol. 209, no. 2, pp. 812–822. https:// doi.org/10.1111/nph.13631Orchestration of hydrogen peroxide and nitric oxide in brassinosteroid‐mediated systemic virus resistance in Nicotiana benthamiana / X. G. Deng [et al.] // Plant J. – 2016. – Vol. 85, N 4. – P. 478–493. https://doi.org/10.1111/tpj.13120Deng X. G., Zhu T., Zou L. J., Han X. Y., Zhou X., Xi D. H., Zhang D. W., Lin H. H. Orchestration of hydrogen peroxide and nitric oxide in brassinosteroid‐mediated systemic virus resistance in Nicotiana benthamiana. Plant Journal, 2016, vol. 85, no. 4, pp. 478–493. https://doi.org/10.1111/tpj.13120Combined seed and foliar pre-treatments with exogenous methyl jasmonate and salicylic acid mitigate drought-induced stress in maize / N. Tayyab [et al.] // PLoS ONE. – 2020. – Vol. 15, N 5. – P. e0232269. https://doi.org/10.1371/journal. pone.0232269Tayyab N., Naz R., Yasmin H., Nosheen A., Keyani R., Sajjad M., Hassan M. N., Roberts T. H. Combined seed and foliar pre-treatments with exogenous methyl jasmonate and salicylic acid mitigate drought-induced stress in maize. PLoS ONE, 2020, vol. 15, no. 5, p. e0232269. https://doi.org/10.1371/journal.pone.0232269Балюк, Н. В. Защитные реакции инфицированных вирусом y растений картофеля при обработке 24-эпибрассинолидом с салициловой кислотой и метилжасмонатом / Н. В. Балюк, Ж. Н. Калацкая, Н. А. Ламан // Вес. Нац. aкад. навук Беларусі. Сер. біял. навук. – 2023. – Т. 68, № 2. – С. 95–103.Balyuk N. V., Kalatskaya Zh. N., Laman N. A. Protective reactions of potato plants infected with Y virus when treated with 24-epibrassinolide with salicylic acid and methyl jasmonate. Vestsi Natsyyanal’nai akademii navuk Belarusi. Seryya biyalagichnykh navuk = Proceedings of the National Academy of Sciences of Belarus. Biological series, 2023, vol. 68, no. 2, pp. 95–103 (in Russian).Interplay between abiotic (drought) and biotic (virus) stresses in tomato plants / R. Mishra [et al.] // Molec. Plant Pathol. – 2022. – Vol. 23, N 4. – P. 475–488. https://doi.org/10.1111/mpp.13172Mishra R., Shteinberg M., Shkolnik D., Anfoka G., Czosnek H., Gorovits R. Interplay between abiotic (drought) and biotic (virus) stresses in tomato plants. Molecular Plant Pathology, 2022, vol. 23, no. 4, pp. 475–488. https://doi.org/10.1111/ mpp.13172Yarwood, C. E. Virus susceptibility increased by soaking bean leaves in water / C. E. Yarwood // Plant Dis. Reporter. – 1959. – Vol. 43. – P. 841–844.Yarwood C. E. Virus susceptibility increased by soaking bean leaves in water. Plant Disease Reporter, 1959, vol. 43, pp. 841–844.Reactive oxygen species are involved in brassinosteroid-induced stress tolerance in cucumber / X. J. Xia [et al.] // Plant Physiol. – 2009. – Vol. 150, N 2. – P. 801–814. https://doi.org/10.1104/pp.109.138230Xia X. J., Wang Y. J., Zhou Y. H., Tao Y., Mao W. H., Shi K., Asami T., Chen Z., Yu J. Q. Reactive oxygen species are involved in brassinosteroid-induced stress tolerance in cucumber. Plant Physiology, 2009, vol. 150, no. 2, pp. 801–814. https:// doi.org/10.1104/pp.109.138230Brassinosteroids suppress rice defense against root-knot nematodes through antagonism with the jasmonate pathway / K. Nahar [et al.] // Molec. Plant-Microbe Interact. – 2013. – Vol. 26, N 1. – P. 106–115. https://doi.org/10.1094/MPMI-05-12- 0108-FINahar K., Kyndt T., Hause B., Höfte M., Gheysen, G. Brassinosteroids suppress rice defense against root-knot nematodes through antagonism with the jasmonate pathway. Molecular Plant-Microbe Interactions, 2013, vol. 26, no. 1, pp. 106– 115. https://doi.org/10.1094/MPMI-05-12-0108-FIYusuf, M. Brassinosteroids: Physiological roles and its signalling in plants / M. Yusuf, T. A. Khan, Q. Fariduddin // Stress Signaling in Plants: Genomics and Proteomics Perspective / ed.: M. Sarwat [et al.]. – New York, 2017. – Vol. 2. – P. 241–260. https://doi.org/10.1007/978-3-319-42183-4_10Yusuf M., Khan T. A., Fariduddin Q. Brassinosteroids: Physiological roles and its signalling in plants. Stress Signaling in Plants: Genomics and Proteomics Perspective. Vol. 2. New York, 2017, pp. 241–260. https://doi.org/10.1007/978-3-319- 42183-4_10Effects of salicylic acid on yield and quality characters of tomato fruit (Lycopersicum esculentum Mill.) / M. Javaheri [et al.] // Int. J. Agric. Crop Sci. – 2012. – Vol. 4, N 16. – P. 1184–1187.Javaheri M., Mashayekhi K., Dadkhah A., Tavallaee F. Z. Effects of salicylic acid on yield and quality characters of tomato fruit (Lycopersicum esculentum Mill.). International Journal of Agriculture and Crop Sciences, 2012, vol. 4, no. 16, pp. 1184–1187.Jasmonic acid and methyl jasmonate modulate growth, photosynthetic activity and expression of photosystem II subunit genes in Brassica oleracea L / G. Sirhindi [et al.] // Sci. Rep. – 2020. – Vol. 10, N 1. – P. 9322–1–14. https://doi.org/10.1038/ s41598-020-65309-1Sirhindi G., Mushtaq R., Gill S. S., Sharma P., Abd Allah E. F., Ahmad P. Jasmonic acid and methyl jasmonate modulate growth, photosynthetic activity and expression of photosystem II subunit genes in Brassica oleracea L. Scientific Reports, 2020, vol. 10, no. 1, pp. 9322–1–14. https://doi.org/10.1038/s41598-020-65309-1Methyl jasmonate and salicylic acid elicitation induces ginsenosides accumulation, enzymatic and nonenzymatic antioxidant in suspension culture Panax ginseng roots in bioreactors / B. M. Ali [et al.] // Plant Cell Rep. – 2006. – Vol. 25, N 6. – P. 613–620. https://doi.org/10.1007/s00299-005-0065-6Ali M. B., Yu K. W., Hahn E. J., Paek K. Y. Methyl jasmonate and salicylic acid elicitation induces ginsenosides accumulation, enzymatic and nonenzymatic antioxidant in suspension culture Panax ginseng roots in bioreactors. Plant Cell Reports, 2006, vol. 25, no. 6, pp. 613–620. https://doi.org/10.1007/s00299-005-0065-6Light-dependent induction of proline biosynthesis by abscisic acid and salt stress is inhibited by brassinosteroid in Arabidopsis / E. Abraham [et al.] // Plant Mol. Biol. – 2003. – Vol. 51. – P. 363–372. https://doi.org/10.1023/A:1022043000516Abrahám E., Rigó G., Székely G., Nagy R., Koncz C., Szabados L. Light-dependent induction of proline biosynthesis by abscisic acid and salt stress is inhibited by brassinosteroid in Arabidopsis. Plant Molecular Biology, 2003, vol. 51, pp. 363–372. https://doi.org/10.1023/A:1022043000516Mohanty, S. K. Physiology of rice tungro virus disease: proline accumulation due to infection / S. K. Mohanty, R. Sridhar // Phys. Plantarum. – 1982. – Vol. 56, N 1. – P. 89–93. https://doi.org/10.1111/j.1399-3054.1982.tb04904.xMohanty S. K., Sridhar R. Physiology of rice tungro virus disease: proline accumulation due to infection. Physiologia Plantarum, 1982, vol. 56, no. 1, pp. 89–93. https://doi.org/10.1111/j.1399-3054.1982.tb04904.x

The authors declare that there are no conflicts of interest present.