vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2023-68-4-315-324vestib-897Research ArticleСтатьиПЦР в режиме реального времени для индикации представителей порядка Chlamydiales: необходимость разработки и основные аналитические характеристики методаReal-time PCR for indication of representatives of Chlamydiales order: needs for development and main analytical characteristics of the methodКапустинаЮ. М.KapustinaYu. M.

Юлия Михайловна Капустина, аспирант, мл. науч. сотрудник

220114

ул. Филимонова, 23

Минск

Yulia M. Kapustina, Postgraduate student, Junior Researcher

220114

23, Filimonov Str.

Minsk

kapustinajm@gmail.comРубаникЛ. В.RubanikL. V.

Людмила Владимировна Рубаник, канд. биол. наук, доцент, заведующий лабораторией

220114

ул. Филимонова, 23

Минск

Lyudmila V. Rubanik, Ph. D. (Biol.), Associate Professor, Head of the Laboratory

220114

23, Filimonov Str.

Minsk

lab.dsbvi@gmail.comШмелеваН. П.ShmelevaN. P.

Наталья Петровна Шмелева, канд. мед. наук, заведующий лабораторией

220114

ул. Филимонова, 23

Минск

Natalia P. Shmeleva, Ph. D. (Med.), Head of the Laboratory

220114

23, Filimonov Str.

Minsk

shmelevanataliya@mail.ruСивецН. В.SivetsN. V.

Наталья Валерьевна Сивец, науч. сотрудник

220114

ул. Филимонова, 23

Минск

Natalia V. Sivets, Researcher

220114

23, Filimonov Str.

Minsk

sivets_n@mail.ruГригорьеваЕ. Е.GrigorievaE. E.

Елена Евгеньевна Григорьева, канд. биол. наук, вед. науч. сотрудник

220114

ул. Филимонова, 23

Минск

Elena E. Grigorieva, Ph. D. (Biol.), Leading Researcher

220114

23, Filimonov Str.

Minsk

grigus@mail.ruРеспубликанский научно-практический центр эпидемиологии и микробиологииRepublican Scientific and Practical Center of Epidemiology and Microbiology202329102023684315324Copyright © Капустина Ю.М., Рубаник Л.В., Шмелева Н.П., Сивец Н.В., Григорьева Е.Е., 20232023Капустина Ю.М., Рубаник Л.В., Шмелева Н.П., Сивец Н.В., Григорьева Е.Е.Kapustina Y.M., Rubanik L.V., Shmeleva N.P., Sivets N.V., Grigorieva E.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/897

   Заболевания органов дыхания занимают доминирующее положение в структуре болезней, этиология которых зачастую остается невыясненной. Во многом это обусловлено недостаточной изученностью всего спектра патогенов, вызывающих респираторные заболевания, а также отсутствием подходов к их индикации и идентификации. Благодаря постоянному прогрессу молекулярной биологии идентифицирован ряд родственных хламидиям бактерий, включая хламидияподобные микроорганизмы, часть из которых связана с респираторными заболеваниями человека. Роль атипичных возбудителей, таких как хламидияподобные микроорганизмы Parachlamydia acanthamoebae и Simkania negevensis, в патологии респираторного тракта человека активно изучается. Разработан и оптимизирован метод на основе ПЦР в режиме реального времени, который способен выявлять ДНК как хламидий, так и хламидияподобных микроорганизмов (Parachlamydia acanthamoebae и Simkania negevensis). Метод характеризуется высокой специфичностью и воспроизводимостью, а также аналитической чувствительностью на уровне 8,7·103 ГЭ/мл. Его применение даст возможность оценить вклад данных атипичных возбудителей в этиологическую структуру инфекционных заболеваний респираторного тракта.

   Respiratory diseases occupy a dominant position in the structure of morbidity, often the etiology of which remains unclear. This situation is largely due to the lack of knowledge of the entire spectrum of pathogens that cause respiratory diseases, as well as the lack of approaches to their indication and identification. Thanks to constant progress of molecular biology, a number of bacteria related to chlamydia have been identified, some of which are associated with human respiratory diseases, including chlamydia-like microorganisms. The role of atypical pathogens such as chlamydia-like microorganisms Parachlamydia acanthamoebae, and Simkania negevensis in the pathology of the human respiratory tract is still not fully understood. A real-time PCR-based method has been developed and optimized. It is capable of detecting the DNA of both chlamydia and chlamydia-like microorganisms (Parachlamydia acanthamoebae and Simkania negevensis). The method is characterized by high specificity and reproducibility, as well as by analytical sensitivity at a level of 8.7∙103 GE/ml. Its use will make it possible to assess the contribution of these atypical pathogens to the etiological structure of infectious diseases of the respiratory tract.

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The authors declare that there are no conflicts of interest present.