vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2023-68-3-224-233vestib-890Research ArticleСтатьиВлияние температурного шока на эффективность синтеза поверхностноактивных соединений бактериями Rhodococcus pyridinivorans 5ApInfluence of a temperature shock on the synthesis efficiency of surfactants by Rhodococcus pyridinivorans 5Ap bacteriahttps://orcid.org/0000-0003-3643-1366БукляревичА. А.BukliarevichH.

Букляревич Анна Александровна – младший научный cотрудник.

Пр. Независимости, 4, 220030, Минск

Hanna A. Bukliarevich – Junior Researcher.

4, Nezavisimosti Ave., 220030, Minsk

bukliarevich@bsu.byhttps://orcid.org/0000-0002-8623-5083ТитокМ. А.TitokM. A.

Титок Марина Алексеевна – доктор биологических наук, профессор.

Пр. Независимости, 4, 220030, Минск

Marina A. Titok – D. Sc. (Biol.), Professor.

4, Nezavisimosti Ave., 220030, Minsk

titok@bsu.byБелорусский государственный университетBelarusian State University202330072023683224233Copyright © Букляревич А.А., Титок М.А., 20232023Букляревич А.А., Титок М.А.Bukliarevich H., Titok M.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/890

В результате проведенного исследования установлено, что синтез поверхностно-активных соединений бактериями R. pyridinivorans 5Ар можно повысить путем воздействия на них кратковременным температурным шоком (55 °С в течение 20 мин) через 24 ч выращивания в минимальной солевой среде, содержащей мелассу (3 %) и гексадекан (2 %) (индекс эмульгирования увеличивается на 9 %). При данном режиме культивирования регистрировали активацию экспрессии генетических детерминант, которые кодируют глобальные регуляторы клеточного метаболизма и выполняют в том числе защитную функцию при стрессе. В частности, показано увеличение в 90,8 ра за количества мРНК, определяющей синтез альтернативного транскрипционного фактора SigH, а также генов, содержащих в промоторах сайты его связывания и кодирующих синтез кошаперона (ген fmdB), шаперона (ген hsp22.5) и тиоредоксинредуктазы (ген trxB) (соответственно в 59,3; 81,1 и 73,1 раза). Кроме того, показана активация транскрипции генов groEL1, groEL2 и dnaJ, обеспечивающая увеличение синтеза белков теплового шока (в 2,2; 2,6 и 4,4 раза соответственно). Полученные данные позволяют предположить, что увеличение синтеза альтернативного фактора сигма Н, активирующего защитный клеточный метаболизм, а также структурных белков теплового шока при кратковременном температурном стрессе приводит к возрастанию продукции поверхностно-активных соединений, что может быть использовано при оптимизации синтеза данных вторичных метаболитов для биотехнологического использования.

It was found that the synthesis of surfactants by R. pyridinivorans 5Ap bacteria can be increased by exposing them to a short temperature shock (55 °C for 20 minutes) after 24 hour cultivation in a minimal medium containing molasses (3 %) and hexadecane (2 %) (9 % increase in the emulsification index). Gene activation encoding global regulators of cell metabolism, including those performing a protective function under stress, was recorded in this cultivation mode. In particular, the mRNA amount determining the synthesis of the alternative transcription factor SigH increased 90.8 times and containing in the promoters its binding sites fmdB cochaperone – 59.3 times, hsp22.5 chaperone – 81.1 times, and the trxB gene encoding thioredoxin reductase – 73.1 times. In addition, it was shown that the transcriptional activation of groEL1, groEL2 and dnaJ genes determining the synthesis of heat shock proteins was 2.2, 2.6 and 4.4 times, respectively. The obtained results suggest that an increase in the alternative factor sigma H synthesis, which activates protective cellular metabolism, as well as structural, heat shock proteins under short temperature stress, leads to an increased production of surfactants, which can be used to optimize the synthesis of these secondary metabolites for biotechnological use.

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The authors declare that there are no conflicts of interest present.