vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2023-68-3-206-212vestib-884Research ArticleСтатьиПолучение комплексов хитозана с 3′,5′-циклическим диаденозинмонофосфатомProduction of chitosan complexes with 3ʹ,5ʹ-сyclic diadenosine monophosphateВинтерМ. А.VinterM. A.

Винтер Маргарита Андреевна – аспирант.

Ул. Купревича, 2, 220141, Минск

Margarita A. Vinter – Postgraduate student.

2, Kuprevich Str., 220141, Minsk

rita.vinter.abc@gmail.comКазловскийИ. С.KazlouskiI. S.

Казловский Илья Сергеевич – кандидат биологических наук, старший научный сотрудник.

Ул. Купревича, 2, 220141, Минск

Illia S. Kazlouski – Ph. D. (Biol.), Senior Researcher.

2, Kuprevich Str., 220141, Minsk

kazlouski.illia@gmail.comhttps://orcid.org/0000-0003-2401-2586ЗинченкоА. И.ZinchenkoA. I.

Зинченко Анатолий Иванович – член-корреспондент, доктор биологических наук, профессор, заведующий лабораторией.

Ул. Купре вича, 2, 220141, Минск

Anatolу I. Zinchenko – Corresponding Member, D. Sc. (Biol.), Professor, Head of the Laboratory.

2, Kuprevich Str., 220141, Minsk

zinch@mbio.bas-net.byИнститут микробиологии НАН БеларусиInstitute of Microbiology of the National Academy of Sciences of Belarus202329072023683206212Copyright © Винтер М.А., Казловский И.С., Зинченко А.И., 20232023Винтер М.А., Казловский И.С., Зинченко А.И.Vinter M.A., Kazlouski I.S., Zinchenko A.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/884

С целью решения проблемы доставки фармакологически перспективного 3ʹ,5ʹ-циклического диаденозинмонофосфата (цикло-диАМФ) к клеткам-мишеням в организме человека и животных впервые методом ионотропного гелеобразования синтезированы комплексы указанного динуклеотида с природным полимером – хитозаном. Установлено, что степень связывания цикло-диАМФ с этим биополимерным носителем достигает 60 %, при этом емкость полученных комплексов в отношении динуклеотида составляет 800–860 мкг/мг комплекса. Показано также, что цикло-диАМФ способен элюироваться из комплекса с хитозаном в цитрат-фосфатный буфер (рН 7,4) на 36 % за 21 ч. Полученные результаты свидетельствуют о возможности использования комплекса цикло-диАМФ с хитозаном для пролонгированной доставки изученного циклического динуклеотида в клетки-мишени.

To solve the problem of delivering pharmacologically promising 3ʹ,5ʹ-cyclic diadenosine monophosphate (cyclo-diAMP) to target cells in humans and animals, the complexes of the above-mentioned dinucleotide with natural polymer – chitosan were originally synthesized by ionotropic gelation technique. It was found that the binding degree of cyclo-diAMP to this biopolymeric carrier reaches 60 %; wherein the capacity of the obtained complexes with respect to the dinucleotide is 800–860 µg/mg of the produced complex. Cyclo-diAMP has also been shown to elute from the chitosan complex to the citrate-phosphate buffer (pH 7.4) up to 36 % by 21 hours. The obtained results testify in favor of potential application of cyclodiAMP complex with chitosan for prolonged delivery of the studied cyclic dinucleotide to target cells.

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The authors declare that there are no conflicts of interest present.