vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2023-68-3-197-205vestib-883Research ArticleСтатьиФизиологические изменения митохондрий при сахарном диабете и фармакологические подходы по их устранениюPhysiological changes of mitochondria under the diabetes condition and pharmacological approaches to eliminate themГайибовУ. Г.GayibovU. G.

Гайибов Улугбек Гаппарджанович – кандидат биологических наук, старший научный сотрудник.

Ул. Шахрисабз, 5, 100060, Ташкент

Ulugbek G. Gayibov – Ph. D. (Biol.), Senior Researcher.

5, Shakhrisabz Str., 100060, Tashkent

gayibov.ulugbek@gmail.comРузибоевХ. С.RuziboevH. S.

Рузибоев Хайдарали Собиржонович – кандидат биологических наук, доцент.

700174, г. Ташкент, ВУЗ городок

Haydarali S. Ruziboev – Ph. D. (Biol.), Associate Pro fessor.

700174, University campus, 700174, Tashkent

ЕралиевМ. Э.EralievM. Y.

Ералиев Муродилла Эргашевич – заведующий отде лением. Отделение хирургической реанимации.

Малая кольцевая дорога № 2, Ташкент, Намаган

Murodilla Y. Eraliev – Head of Department.

Small Ring Road No. 2, Tashkent

ПозиловМ. К.PozilovM. K.

Мамуржон Комилжонович Позилов – кандидат биологических наук, доцент.

700174, ВУЗ городок, Ташкент

Mamurjon K. Pozilov – Ph. D. (Biol.), Associate Professor.

University campus, 700174, Tashkent

mamurjon2281@mail.ruАсраровМ. И.AsrarovM. I.

Асраров Музаффар Исламович – доктор биологических наук, профессор, заместитель директора.

Ул. Талабалар, 174, Ташкент

Muzaffar I. Asrarov – D. Sc. (Biol.), Professor, Deputy Director.

174, Talabalar Str., Tashkent

asrarov54@mail.ruАбдулладжановаН. Г.AbdulladjanovaN. G.

Абдулладжанова Нодира Гулямжановна – доктор химических наук, ведущий научный сотрудник.

Ул. Мирзо Улугбека, 83, 100125, Ташкент

Nodira G. Abdulladjanova – D. Sc. (Chem.), Leading Researcher.

83, Mirzo Ulugbek, 100125, Tashkent

anodira73@rambler.ruСянданьЧ.XiandanCh.

Сяндань Чэнь – аспирант.

Ул. Долгобродская, 23/1, 220070, Минск

Chen Xiandan – Postgraduate student.

23/1, Dolgobrodskaya Str., 220070, Minsk

dan836250811@gmail.comСысаА. Г.SysaA. G.

Алексей Григорьевич Сыса – кандидат химических наук, доцент.

Ул. Долгобродская, 23/1, 220070, Минск

Aliaksei G. Sysa – Ph. D. (Сhem.), Associate Professor.

23/1, Dolgobrodskaya Str., 220070, Minsk

aliaksei.sysa@iseu.byИнститут биоорганической химии имени академика А.С. Садыкова Академии наук Республики УзбекистанInstitute of Bioorganic Chemistry named after acad. A.S. Sadykov, Academy of Sciences of UzbekistanНациональный университет Узбекистана имени Мирзо УлугбекаNational University of Uzbekistan named after M. UlugbekРеспубликанский научный Центр экстренной медицинской помощиDepartment of surgical resuscitation of Namangan branch of the Republican Scientific Center for Emergency Medical CareИнститут биофизики и биохимии при Национальном университете Узбекистана имени Мирзо УлугбекаInstitute of Biophysics and Biochemistry under the National University of Uzbekistan named after Mirzo UlugbekМеждународный государственный экологический институт им. А.Д. Сахарова Белорусского государственного университетаInternational Sakharov Environmental Institute of Belarusian State University202329072023683197205Copyright © Гайибов У.Г., Рузибоев Х.С., Ералиев М.Э., Позилов М.К., Асраров М.И., Абдулладжанова Н.Г., Сяндань Ч., Сыса А.Г., 20232023Гайибов У.Г., Рузибоев Х.С., Ералиев М.Э., Позилов М.К., Асраров М.И., Абдулладжанова Н.Г., Сяндань Ч., Сыса А.Г.Gayibov U.G., Ruziboev H.S., Eraliev M.Y., Pozilov M.K., Asrarov M.I., Abdulladjanova N.G., Xiandan C., Sysa A.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/883

Изучено состояние перекисного окисления липидов (ПОЛ), дыхания и окислительного фосфорилирования, митохондриальной Са2+-зависимой поры (mPTP) и антирадикальной активности митохондрий печени крыс при диабете, индуцированном стрептозотоцином (STZ). Рассмотрены пути коррекции выявленных повреждений митохондриальных мембран с использованием полифенола госситана, выделенного из хлопчатника Gossypium hirsutum L. Показано, что скорость дыхания митохондрий печени в состояниях V3 и V4 при STZ-индуцированном диабете увеличивается, что выражается в значительном снижении коэффициентов дыхательного контроля (V3/V4) и фосфорилирования (ADP/O). Полученные данные свидетельствуют о том, что во время STZ-индуцированного диабета происходит разобщение дыхания и окислительного фосфорилирования. Показано, что в условиях STZ-индуцированного диабета скорость набухания митохондрий печени выше, чем у интактных крыс, что предполагает открытое состояние mPTP. Госситан нормализует динамику mPTP, снижая эффекты STZ на митохондрии печени. Пози тивное влияние госситана (пероральная доза составляет 10 мг/кг массы тела в течение 8 дней) может быть опосредовано также антиоксидантными свойствами в отношении ПОЛ.

The state of lipid peroxidation (LPO), respiration and oxidative phosphorylation, mitochondrial permeability transition pore (mPTP), and the antiradical activity of rat liver mitochondria in the streptozotocin (STZ)-induced diabetes condition were studied with the consideration of the ways of correcting detected membrane damages using gossitan isolated from the cotton plant Gossypium hirsutum L. It was shown that the rate of respiration of liver mitochondria in states V3 and V4 increases during STZ-induced diabetes, which significantly reduces the respiratory control (RC) and ADP/O coefficients in comparison with the control. The findings suggest that the uncoupling of respiration and oxidative phosphorylation takes place during STZ-induced diabetes. It was shown that in the STZ-induced diabetes condition, the rate of swelling of rat liver mitochondria is higher than that of the healthy ones; this means that mPTP of rat liver mitochondria is in the open state. Gossitan recovers mPTP to the normal condition, thereby removing the STZ effect on mitochondria. Gossitan (a personal dose is 10 mg/kg of body weight, during 8 days) eliminates the detected functional disorders of rat liver mitochondria, probably due to its antioxidant properties.

полифенолымитохондрииантирадикальная активностьантиоксидантная активностьсахарный диабетpolyphenol compoundsmitochondriaantiradical activityantioxidant activitydiabetesРабота выполнена при поддержке гранта Академии наук ФА-Ф5-Т084. Авторы благодарят заведующего лабораторией экспериментальной техноло гии Института биоорганической химии АН РУз проф. С. М. Мавлянову за любезно предоставленное для исследований полифенольное соединение госситанThe work was supported by the grant FA-F5-TO84 of the Academy of Sciences. The authors are very grateful to Prof. S. M. Mavlyanov, Head of the Experimental Technology Laboratory of the Institute of Bioorga nic Chemistry of the Academy of Sciences of the Republic of Uzbekistan for polyphenol gossitan kindly given for re searchReferencesOliveira P. J. Cardiac mitochondrial alterations observed in hyperglycaemic rats – what can we learn from cell biology? Current Diabetes Reviews, 2005, vol. 1, no. 1, pp. 11–21. https://doi.org/10.2174/1573399052952578Oliveira P. J. Cardiac mitochondrial alterations observed in hyperglycaemic rats – what can we learn from cell biology? Current Diabetes Reviews, 2005, vol. 1, no. 1, pp. 11–21. https://doi.org/10.2174/1573399052952578Hajiaghaalipour F., Khalilpourfarshbafi M., Arya A. Modulation of glucose transporter protein by dietary flavonoids in type 2 diabetes mellitus. International Journal of Biological Sciences, 2015, vol. 11, no. 5, pp. 508–524. https://doi.org/10.7150/ijbs.11241Hajiaghaalipour F., Khalilpourfarshbafi M., Arya A. Modulation of glucose transporter protein by dietary flavonoids in type 2 diabetes mellitus. International Journal of Biological Sciences, 2015, vol. 11, no. 5, pp. 508–524. https://doi.org/10.7150/ijbs.11241Kane G. C., Behfar A., Yamada S., Terzic C. P., O’cochlain F., Reyes S., Dzeja P. P., Miki T., Seino S., Terzic A. ATPsensitive K+ channel knockout compromises the metabolic benefit of exercise training, resulting in cardiac deficits. Diabetes, 2004, vol. 53, no. 3, pp. 169–175. https://doi.org/10.2337/diabetes.53.suppl_3.s169Kane G. C., Behfar A., Yamada S., Terzic C. P., O’cochlain F., Reyes S., Dzeja P. P., Miki T., Seino S., Terzic A. ATPsensitive K+ channel knockout compromises the metabolic benefit of exercise training, resulting in cardiac deficits. Diabetes, 2004, vol. 53, no. 3, pp. 169–175. https://doi.org/10.2337/diabetes.53.suppl_3.s169Rolo A. P., Palmeira C. M. Diabetes and mitochondrial function: Role of hyperglycemia and oxidative stress. Toxicology and Applied Pharmacology, 2006, vol. 212, no. 2, pp. 167–178. https://doi.org/10.1016/j.taap.2006.01.003Rolo A. P., Palmeira C. M. Diabetes and mitochondrial function: Role of hyperglycemia and oxidative stress. Toxicology and Applied Pharmacology, 2006, vol. 212, no. 2, pp. 167–178. https://doi.org/10.1016/j.taap.2006.01.003Sakamoto J., Barr R. L., Kavanagh K. M., Lopaschuk G. D. Contribution of malonyl-CoA decarboxylase to the high fatty acid oxidation rates seen in the diabetic heart. American Journal of Physiology-Heart and Circulatory Physiology, 2000, vol. 278, no. 4, pp. 1196–1204. https://doi.org/10.1152/ajpheart.2000.278.4.H1196Sakamoto J., Barr R. L., Kavanagh K. M., Lopaschuk G. D. Contribution of malonyl-CoA decarboxylase to the high fatty acid oxidation rates seen in the diabetic heart. American Journal of Physiology-Heart and Circulatory Physiology, 2000, vol. 278, no. 4, pp. 1196–1204. https://doi.org/10.1152/ajpheart.2000.278.4.H1196Asrarov M. I., Pozilov M. K., Ergaschev N. A., Rachmatullaeva M. M. The influence of the hypoglycemic agent glycorazmulin on the functional state of mitochondria in the rats with streptozotocin-induced diabetes. Problems of Endocrinology, 2014, vol. 60, no. 3, pp. 38–42. https://doi.org/10.14341/probl201460338-42Asrarov M. I., Pozilov M. K., Ergaschev N. A., Rachmatullaeva M. M. The influence of the hypoglycemic agent glycorazmulin on the functional state of mitochondria in the rats with streptozotocin-induced diabetes. Problems of Endocrinology, 2014, vol. 60, no. 3, pp. 38–42. https://doi.org/10.14341/probl201460338-42Salikhov S. I., Mavlyanov S., Abdulladjanova N. G., Pirniyazov A. J., Dalimov D. N., Salakhutdinov B. A., Kurmukov A. G. Polyphenols of some tannin containing plants and creation on their base drug remedies. New Research on Biotechnology and Medicine, 2006, pp. 109–117.Salikhov S. I., Mavlyanov S., Abdulladjanova N. G., Pirniyazov A. J., Dalimov D. N., Salakhutdinov B. A., Kurmukov A. G. Polyphenols of some tannin containing plants and creation on their base drug remedies. New Research on Biotechnology and Medicine, 2006, pp. 109–117.Lenzen S. The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia, 2008, vol. 51, pp. 216–226. https://doi.org/10.1007/s00125-007-0886-7Lenzen S. The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia, 2008, vol. 51, pp. 216–226. https://doi.org/10.1007/s00125-007-0886-7Schneider W. C., Hageboom G. H., Pallade G. E. Cytochemical studies of mammalian tissues; isolation of intact mitochondria from rat liver; some biochemical properties of mitochondria and submicroscopic particulate material. Journal of Biological Chemistry, 1948, vol. 172, no. 2, pp. 619–635.Schneider W. C., Hageboom G. H., Pallade G. E. Cytochemical studies of mammalian tissues; isolation of intact mitochondria from rat liver; some biochemical properties of mitochondria and submicroscopic particulate material. Journal of Biological Chemistry, 1948, vol. 172, no. 2, pp. 619–635.Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Analytical Biochemistry, 1977, vol. 83, no. 2, pp. 346–356. https://doi.org/10.1016/0003-2697(77)90043-4Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Analytical Biochemistry, 1977, vol. 83, no. 2, pp. 346–356. https://doi.org/10.1016/0003-2697(77)90043-4He L., Lemasters J. J. Heat shock suppresses the permeability transition in rat liver mitochondria. Journal of Biological Chemistry, 2003, vol. 278, no. 19, pp. 16755–16760. https://doi.org/10.1074/jbc.M300153200He L., Lemasters J. J. Heat shock suppresses the permeability transition in rat liver mitochondria. Journal of Biological Chemistry, 2003, vol. 278, no. 19, pp. 16755–16760. https://doi.org/10.1074/jbc.M300153200Chance B., Williams G. R. Respiratory enzymes in oxidative phosphorylation. III. The steady state. Journal of Biological Chemistry, 1955, vol. 217, no. 1, pp. 409–427.Chance B., Williams G. R. Respiratory enzymes in oxidative phosphorylation. III. The steady state. Journal of Biological Chemistry, 1955, vol. 217, no. 1, pp. 409–427.Boldyrev A. A., Kotelevtsev S. V., Lanio M., Al’vares K., Peres P. Introduction to biomembranology. Moscow, Moscow State University Publishing House, 1990. 208 p. (in Russian).Boldyrev A. A., Kotelevtsev S. V., Lanio M., Al’vares K., Peres P. Introduction to biomembranology. Moscow, Moscow State University Publishing House, 1990. 208 p. (in Russian).Marinova G., Batchvarov V. Evaluation of the methods for determination of the free radical scavenging activity by DPPH. Bulgarian Journal of Agricultural Science, 2011, vol. 17, pp. 11–24.Marinova G., Batchvarov V. Evaluation of the methods for determination of the free radical scavenging activity by DPPH. Bulgarian Journal of Agricultural Science, 2011, vol. 17, pp. 11–24.Pozilov M. K., Ernazarov Z. M., Afzalova S. A., Asrarov M. I., Ergashev N. A., Komilov B. J. Effect of the plant flavonoid luteolin on a mitochondrial function in the streptozotocin-induced diabetic rats. American Journal of Biomedical and Life Sciences, 2020, vol. 8, no. 6, pp. 220–224. https://doi.org/10.11648/j.ajbls.20200806.15Pozilov M. K., Ernazarov Z. M., Afzalova S. A., Asrarov M. I., Ergashev N. A., Komilov B. J. Effect of the plant flavonoid luteolin on a mitochondrial function in the streptozotocin-induced diabetic rats. American Journal of Biomedical and Life Sciences, 2020, vol. 8, no. 6, pp. 220–224. https://doi.org/10.11648/j.ajbls.20200806.15Vengerovskii A. I., Khazanov V. A., Eskina K. A., Vasil’ev K. Yu. Effects of silymarin (hepatoprotector) and succinic acid (bioenergy regulator) on metabolic disorders in experimental diabetes mellitus. Byulleten’ eksperimental’noi biologii i meditsiny [Bulletin of Experimental Biology and Medicine], 2007, vol. 144, no. 1, pp. 53–56.Vengerovskii A. I., Khazanov V. A., Eskina K. A., Vasil’ev K. Yu. Effects of silymarin (hepatoprotector) and succinic acid (bioenergy regulator) on metabolic disorders in experimental diabetes mellitus. Byulleten’ eksperimental’noi biologii i meditsiny [Bulletin of Experimental Biology and Medicine], 2007, vol. 144, no. 1, pp. 53–56.Mustafakulov M. A., Rakhimov R. N., Saatov T. S. Study of antioxidant properties of polyphenols in diabetes model Sovremennye problemy genetiki, genomiki i biotekhnologii: sbornik tezisov respublikanskoi nauchnoi konferentsii (Tashkent, 16 maya 2019 goda) [Modern problems of genetics, genomics and biotechnology: collection of abstracts of the republican scientific conference (Tashkent, May 16, 2019)]. Tashkent, 2019, рp. 152–154 (in Uzbek).Mustafakulov M. A., Rakhimov R. N., Saatov T. S. Study of antioxidant properties of polyphenols in diabetes model Sovremennye problemy genetiki, genomiki i biotekhnologii: sbornik tezisov respublikanskoi nauchnoi konferentsii (Tashkent, 16 maya 2019 goda) [Modern problems of genetics, genomics and biotechnology: collection of abstracts of the republican scientific conference (Tashkent, May 16, 2019)]. Tashkent, 2019, рp. 152–154 (in Uzbek).Raza H., John A., Howarth F. C. Increased oxidative stress and mitochondrial dysfunction in zucker diabetic rat liver and brain. Cellular Physiology and Biochemistry, 2015, vol. 35, no. 3, pp. 1241–1251. https://doi.org/10.1159/000373947Raza H., John A., Howarth F. C. Increased oxidative stress and mitochondrial dysfunction in zucker diabetic rat liver and brain. Cellular Physiology and Biochemistry, 2015, vol. 35, no. 3, pp. 1241–1251. https://doi.org/10.1159/000373947Giacco F., Brownlee M. Oxidative stress and diabetic complications. Circulation Research, 2010, vol. 107, no. 9, pp. 1058–1070. https://doi.org/10.1161/CIRCRESAHA.110.223545Giacco F., Brownlee M. Oxidative stress and diabetic complications. Circulation Research, 2010, vol. 107, no. 9, pp. 1058–1070. https://doi.org/10.1161/CIRCRESAHA.110.223545Ferreira F. M., Palmeira C. M., Seic R., Moreno A. J., Santos M. S. Diabetes and mitochondrial bioenergetics: alterations with age. Journal of Biochemical and Molecular Toxicology, 2003, vol. 17, no. 4, pp. 214–222. https://doi.org/10.1002/jbt.10081Ferreira F. M., Palmeira C. M., Seic R., Moreno A. J., Santos M. S. Diabetes and mitochondrial bioenergetics: alterations with age. Journal of Biochemical and Molecular Toxicology, 2003, vol. 17, no. 4, pp. 214–222. https://doi.org/10.1002/jbt.10081Gayibova S. N., Ivanišová E., Árvay J., Hŕstková M., Slávik M., Petrová J., Hleba L., Tóth N., Kačániová M., Ari- pov T. F. In vitro screening of antioxidant and antimicrobial activities of medicinal plants growing in Slovakia. Journal of Microbiology, Biotechnology and Food Sciences, 2019, vol. 8, no. 6, pp. 1276–1280. https://doi.org/10.15414/jmbfs.2019.8.6.1281-1289Gayibova S. N., Ivanišová E., Árvay J., Hŕstková M., Slávik M., Petrová J., Hleba L., Tóth N., Kačániová M., Ari- pov T. F. In vitro screening of antioxidant and antimicrobial activities of medicinal plants growing in Slovakia. Journal of Microbiology, Biotechnology and Food Sciences, 2019, vol. 8, no. 6, pp. 1276–1280. https://doi.org/10.15414/jmbfs.2019.8.6.1281-1289Mierzliak J., Kostyn K., Kulma A. Flavonoids as important molecules af plant interaction with the environment. Molecules, 2014, vol. 16, no. 10, pp. 16240–16265. https://doi.org/10.3390/molecules191016240Mierzliak J., Kostyn K., Kulma A. Flavonoids as important molecules af plant interaction with the environment. Molecules, 2014, vol. 16, no. 10, pp. 16240–16265. https://doi.org/10.3390/molecules191016240Singh V., Mandhania S., Pal A., Kaur T., Banakar P., Sankaranarayanan K., Arya S. S., Malik K., Datten R. Morphophysiological and biochemical responses of cotton (Gossypium hirsutum L.) genotypes upon sucking insect-pest infestations. Physiology and Molecular Biology of Plants, 2022, vol. 28, no. 11–12, pp. 2023–2039. https://doi.org/10.1007/s12298-022-01253-wSingh V., Mandhania S., Pal A., Kaur T., Banakar P., Sankaranarayanan K., Arya S. S., Malik K., Datten R. Morphophysiological and biochemical responses of cotton (Gossypium hirsutum L.) genotypes upon sucking insect-pest infestations. Physiology and Molecular Biology of Plants, 2022, vol. 28, no. 11–12, pp. 2023–2039. https://doi.org/10.1007/s12298-022-01253-wPietta P.-G. Flavonoids as antioxidants. Journal of Natural Products, 2000, vol. 63, no. 7, pp. 1035–1042. https://doi.org/10.1021/np9904509Pietta P.-G. Flavonoids as antioxidants. Journal of Natural Products, 2000, vol. 63, no. 7, pp. 1035–1042. https://doi.org/10.1021/np9904509Parker L. Flavonoids and other polyphenols. Methods and Enzymology, 2001, vol. 335, pp. 15–34.Parker L. Flavonoids and other polyphenols. Methods and Enzymology, 2001, vol. 335, pp. 15–34.Gayibov U. G., Komilov E. J., Rakhimov R. N., Ergashev N. A., Abdullajanova N. G., Asrorov M. I., Aripov T. F. Influence of new polyphenol compound from euphorbia plant on mitochondrial function. Journal of Microbiology, Biotechnology and Food Sciences, 2019, vol. 8, no. 4, pp. 1021–1025. https://doi.org/10.15414/jmbfs.2019.8.4.1021-1025Gayibov U. G., Komilov E. J., Rakhimov R. N., Ergashev N. A., Abdullajanova N. G., Asrorov M. I., Aripov T. F. Influence of new polyphenol compound from euphorbia plant on mitochondrial function. Journal of Microbiology, Biotechnology and Food Sciences, 2019, vol. 8, no. 4, pp. 1021–1025. https://doi.org/10.15414/jmbfs.2019.8.4.1021-1025Gaiibov U. G., Komilov E. D., Ergashev N. A., Rakhimov R. N., Abdullazhanova N. G., Asrarov M. I., Aripov T. F. Antioxidant and membrane-active properties of 1,4,6 tri-o-galloyl-2,3-valoneyl-β-d-glucose. European Journal of Medicine. Series B, 2018, vol. 5, no. 1, pp. 3–15. https://doi.org/10.13187/ejm.s.b.2018.1.3Gaiibov U. G., Komilov E. D., Ergashev N. A., Rakhimov R. N., Abdullazhanova N. G., Asrarov M. I., Aripov T. F. Antioxidant and membrane-active properties of 1,4,6 tri-o-galloyl-2,3-valoneyl-β-d-glucose. European Journal of Medicine. Series B, 2018, vol. 5, no. 1, pp. 3–15. https://doi.org/10.13187/ejm.s.b.2018.1.3Salakhutdinov B. A., Gayibov U. G., Tilyabaev K. Z. Antioxidant and membrane activities of gossypol and its derivatives. Special edition. Uzbekskii biologicheskii zhurnal = Uzbek biological journal, 2010, pp. 83–87 (in Russian).Salakhutdinov B. A., Gayibov U. G., Tilyabaev K. Z. Antioxidant and membrane activities of gossypol and its derivatives. Special edition. Uzbekskii biologicheskii zhurnal = Uzbek biological journal, 2010, pp. 83–87 (in Russian).Fruehauf J. P., Meyskens F. L. Reactive oxygen species: A breath of life or death? Clinical Cancer Research, 2007, vol. 13, no. 3, pp. 789–796. https://doi.org/10.1158/1078-0432.CCR-06-2082Fruehauf J. P., Meyskens F. L. Reactive oxygen species: A breath of life or death? Clinical Cancer Research, 2007, vol. 13, no. 3, pp. 789–796. https://doi.org/10.1158/1078-0432.CCR-06-2082Pozilov M. K., Asrarov M. I., Urmanova G. U., Eshbakova K. A. Protective effect of salvifolin on liver mitochondrial function in rats with experimental diabetes. European Science Review, 2015, no. 7–8, pp. 3–7.Pozilov M. K., Asrarov M. I., Urmanova G. U., Eshbakova K. A. Protective effect of salvifolin on liver mitochondrial function in rats with experimental diabetes. European Science Review, 2015, no. 7–8, pp. 3–7.Halestrap A. P., McStay G. P., Clarke S. J. The permeability transition pore complex: another view. Biochimie, 2002, vol. 84, no. 2–3, pp. 153–166. https://doi.org/10.1016/s0300-9084(02)01375-5Halestrap A. P., McStay G. P., Clarke S. J. The permeability transition pore complex: another view. Biochimie, 2002, vol. 84, no. 2–3, pp. 153–166. https://doi.org/10.1016/s0300-9084(02)01375-5Haworth R. A., Hunter D. R. The Ca2+-induced membrane transition in mitochondria. II. Nature of the Ca2+ trigger site. Archives of Biochemistry and Biophysics, 1979, vol. 195, no. 2, pp. 460–467. https://doi.org/10.1016/0003-9861(79)90372-2Haworth R. A., Hunter D. R. The Ca2+-induced membrane transition in mitochondria. II. Nature of the Ca2+ trigger site. Archives of Biochemistry and Biophysics, 1979, vol. 195, no. 2, pp. 460–467. https://doi.org/10.1016/0003-9861(79)90372-2Ehigie F. A., Ehigie L. O., Olanlokun O. J., Oyelere F. S., Oyebode O. T., Olorunsogo O. O. Inhibitory effect of the methanol leaf extract of momordica charantiaon liver mitochondrial permeability transition pore opening in alloxan-induced diabetic rats. European Journal of Biomedical and Pharmaceutical Sciences, 2019, vol. 6, no. 8, pp. 73–78.Ehigie F. A., Ehigie L. O., Olanlokun O. J., Oyelere F. S., Oyebode O. T., Olorunsogo O. O. Inhibitory effect of the methanol leaf extract of momordica charantiaon liver mitochondrial permeability transition pore opening in alloxan-induced diabetic rats. European Journal of Biomedical and Pharmaceutical Sciences, 2019, vol. 6, no. 8, pp. 73–78.

The authors declare that there are no conflicts of interest present.