vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2023-68-1-64-74vestib-851Research ArticleСтатьиГаплотипическое разнообразие mtCytb обыкновенной полевки (Microtus arvalis sensu lato) в БеларусиHaplotypic diversity of the mtCytb gene of the common vole (Microtus arvalis sensu lato) in BelarusМашковЕ. И.MashkovE. I.

Машков Евгений Игоревич ‒ мл. науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Evgeniy I. Mashkov ‒ Junior Researcher

27, Akademicheskaya Str., 220072, Minsk

mashkov.evgenii25@gmail.comГайдученкоЕ. С.GajduchenkoH. S.

Гайдученко Елена Сергеевна ‒ канд. биол. наук, вед. науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Helen S. Gajduchenko ‒ Ph. D. (Biol.), Leading Researcher

27, Akademicheskaya Str., 220072, Minsk

gajduchenko@tut.byБорисовЮ. М.BorisovYu. M.

Борисов Юрий Михайлович – д-р биол. наук, вед. науч. сотрудник

Ленинский проспект, 27, 119071, г. Москва

Yury M. Borisov ‒ D. Sc. (Biol.), Leading Researcher

27, Leninski Ave., 119071, Moscow

borisov2693@gmail.comНаучно-практический центр НАН Беларуси по биоресурсамScientific and Practical Center of the National Academy of Sciences of Belarus for BioresourcesИнститут проблем экологии и эволюции им. А. Н. Северцова РАНSevertsov Institute of Ecology and Evolution, Russian Academy of Scences2023110220236816474Copyright © Машков Е.И., Гайдученко Е.С., Борисов Ю.М., 20232023Машков Е.И., Гайдученко Е.С., Борисов Ю.М.Mashkov E.I., Gajduchenko H.S., Borisov Y.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/851

В настоящем исследовании представлены оригинальные данные о генетической вариабельности гена mtCytb обыкновенной полевки (Microtus arvalis) из 18 локалитетов, исследованных на территории Беларуси. Проведен анализ последних филогеографических данных для установления послеледниковой истории колонизации обыкновенной полевки в Центральном регионе Европы. Всего в ходе исследования изучены генетические характеристики 53 особей изучаемого вида, относящихся к одной из шести существующих мтДНК линий. Показано, что популяция вида в Беларуси характеризуется высоким уровнем генетического разнообразия, сравнимым с таковым у других гаплогрупп восточной мтДНК линии. Большое число выявленных гаплотипов мтДНК (n = 41) говорит о высоком уровне полиморфизма фрагментов гена mtCytb Microtus arvalis Беларуси. Анализ распределения указанных гаплотипов обыкновенной полевки по территории Беларуси показал отсутствие связи с флористической специфичностью территорий. Однако распределение по различным ландшафтно-географическим районам, напротив, хорошо соотносится с расположением наиболее удаленных гаплотипов на медианной сети. Предполагается, что около 9000 тыс. лет назад имело место повторное заселение и дальнейшее расселение обыкновенной полевки на современной территории Беларуси в двух направлениях: с юго-востока на запад и северо-запад, а также с севера на юго-восток.

The analysis of the recent phylogeographic studies of Microtus arvalis is carried out to establish the post-glacial history of colonization of the common vole in the Central region of Europe. The authors have presented the original data on the genetic variability of the mtCytb gene of the common vole (Microtus arvalis s. l.) from 18 localities studied on the territory of Belarus. The genetic characteristics of 53 individuals of the common vole belonging to one eastern line of mitochondrial DNA were studied. It is shown that the population of the species in Belarus is characterized by a high level of genetic diversity comparable to that of other haplogroups of the eastern mtDNA line. The level of variability of mitochondrial fragments of the mtCytb gene of the common vole (Microtus arvalis) from Belarus turned outtobeq uite high (Hd ± SDHd = 0.97 ± 0.02), which is manifested in a large number of the identified mtDNA haplotypes (n = 41). The distribution of the identified haplotypes of Microtus arvalis across various landscape-geographical areas correlates well with the location of the most remote haplotypes on the parsimony network. The assumption is given about the re-settlement (about 9000 thousand years ago) and further settlement of Microtus arvalis on the modern territory of Belarus in two directions: from southeast to west and to northwest, as well as from north to southeast.

Microtus arvalisбиотопПЦР-типированиеБеларусьгаплотипmtCytbгаплогруппарефугиумгенетическое разнообразиемитохондриальная ДНКMicrotus arvalisbiotopePCR typingBelarushaplotypemtCytbhaplogrouprefugiumgenetic diversitymitochondrial DNAАвторы выражают признательность П. А. Пакулю за предоставление образцов тканей обыкновенной полевки и И. А. Крищук за помощь в сборе полевого материала. Работа поддержана БРФФИ на 2020–2022 гг. (проект Б20М-062 «Пространственно-биотопическая структура криптических видов обыкновенной полевки (Microtus arvalis s. l.) в центральной и западной Беларуси»).The authors express their gratitude to P. A. Pakul for providing tissue samples of the common vole. I. A. Kryschuk in helping with the collection of field material. The work was supported by the BRFFI for 2020–2022 (project B20M-062 “Spatial and biotopic structure of cryptic species of common vole (Microtus arvalis s. l.) in central and western Belarus”).ReferencesThe last glacial maximum / P. U. 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The authors declare that there are no conflicts of interest present.