vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2022-67-4-359-373vestib-834Research ArticleСтатьиСтруктурно-функциональная организация митохондриального генома фитопатогенного гриба Phoma sp.1Structural and functional organisation of the phytopathogenic fungi Phoma sp.1 mitochondrial genomeПантелеевС. В.PanteleevS. V.

Пантелеев Станислав Викторович – канд. биол. наук, вед. науч. сотрудник

ул. Пролетарская, 71, 246050, г. Гомель

Stanislav V. Panteleev – Ph. D. (Biol.), Leading Researcher

71, Proletarskaya Str., 246050, Gome

stasikdesu@mail.ruМожаровскаяЛ. В.MozharovskayaL. V.

Можаровская Людмила Валентиновна – науч. сотрудник

ул. Пролетарская, 71, 246050, г. Гомель

Ludmila V. Mozharovskaya – Researcher

71, Proletarskaya Str., 246050, Gomel

milamozh@yandex.byКирьяновП. С.KiryanovP. S.

Кирьянов Павел Сергеевич – мл. науч. сотрудник

ул. Пролетарская, 71, 246050, г. Гомель

Pavel S. Kiryanov – Junior Researcher

71, Proletarskaya Str., 246050, Gomel

pkirjanov@yandex.ruКаганД. И.KaganD. I.

Каган Дмитрий Ильич – канд. биол. наук, заведующий лабораторией

ул. Пролетарская, 71, 246050, г. Гомель

Dmitri I. Kagan – Ph. D. (Biol.), Head of the Laboratory

71, Proletarskaya Str., 246050, Gomel, Republic of Belarus

quercus-belarus@mail.ruБарановО. Ю.BaranovO. Yu.

Баранов Олег Юрьевич – член-корреспондент, д-р биол. наук, доцент, заведующий лабораторией

ул. Пролетарская, 71, 246050, г. Гомель

Oleg Yu. Baranov – Corresponding Member, D. Sc. (Biol.), Associate Professor, Head of the Laboratory

71, Proletarskaya Str., 246050, Gomel

betula-belarus@mail.ruИнститут леса НАН БеларусиForest Research Institute of the National Academy of Sciences of Belarus202201112022674359357Copyright © Пантелеев С.В., Можаровская Л.В., Кирьянов П.С., Каган Д.И., Баранов О.Ю., 20222022Пантелеев С.В., Можаровская Л.В., Кирьянов П.С., Каган Д.И., Баранов О.Ю.Panteleev S.V., Mozharovskaya L.V., Kiryanov P.S., Kagan D.I., Baranov O.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/834

В статье изложены результаты исследования митохондриальной ДНК фитопатогена Phoma sp.1 – одного из возбудителей фомоза посадочного материала сосны и ели в лесных питомниках Беларуси. Осуществлены секвенирование и аннотация митохондриона Phoma sp.1 (30 837 н. о., 43 кодирующих локуса), изучены его структурно-функциональные особенности и составлена генетическая карта. Идентифицированы интронные вставки в генах cox3 и nad1, а также описаны потенциальные открытые рамки считывания orf89, orf87, orf76 и orf108 с неустановленной функцией. Проведен сравнительный генетический анализ митохондриона и его отдельных генов в базе данных NCBI BLAST. На основании результатов генетико-таксономического анализа установлена близкая степень родства исследованного фитопатогена Phoma sp.1 с видами рода Didymella, что согласуется с современными научными данными об анаморфно-телеоморфных взаимоотношениях Phoma и Didymella. Показано, что гены мтДНК могут выступать в качестве маркеров для диагностики Phoma и фома-подобных грибов на уровне семейств, родов и видов. В ходе изучения синтении митохондриальных геномов Phoma sp.1 и близкородственных видов выявлены значительные структурные перестройки мтДНК в ходе филогенеза.

The article presents the results of the mitochondrial DNA (30 837 n. r.) sequencing of the phytopathogenic fungi Phoma sp.1 – causative agent of Phoma blight of the pine and spruce plants cultivated in the forest nurseries. Annotation of the Phoma sp.1 mitochondrion showed 43 coding loci. Potential open reading frames (orf89, orf87, orf76 and orf108) and gene introns (cox3, nad1) are described. A comparative single genes analysis in the NCBI GenBank database showed, that Phoma sp.1 belongs to the Didymella spp., which can have Phoma anamorph. It has been shown that mitohondrial genes can be used as DNA markers for the diagnosis of Phoma and phoma-like fungi. Analysis of the mitochondrial synthenia of Phoma sp.1 and a related species (including phoma-like fungi), revealed significant structural rearrangements in mtDNA during phylogenesis.

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The authors declare that there are no conflicts of interest present.