vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2022-67-3-274-284vestib-820Research ArticleСтатьиМолекулярно-генетический анализ детерминант, кодирующих β-галактозидазы бактерийМolecular-genetic analysis of determinants encoding β-galactosidases of bacteria Bifidobacterium longum BIM B-813 DМорозоваА. Н.MorozovaA. N.

Морозова Антонина Николаевна – науч. сотрудник

ул. Купревича, 2, 220141, г. Минск

Antonina N. Morozova – Researcher

2, Kuprevich Str., 220141, Minsk

bifidoby@yandex.byОхремчукА. Э.AkhremchukA. E.

Охремчук Артур Эдуардович – мл. науч. сотрудник

ул. Купревича, 2, 220141, г. Минск

Artur E. Akhremchuk – Junior Researcher

2, Kuprevich Str., 220141, Minsk

okhrem4ukartur@yandex.ruГоловневаН. А.GolovnyovaN. А.

Головнева Наталья Алексеевна – канд. биол. наук, заведующий лабораторией

ул. Купревича, 2, 220141, г. Минск

Natalia A. Golovnyova – Ph. D. (Biol.), Head of the Labo­ ratory

2, Kuprevich Str., 220141, Minsk

bifidoby@yandex.byИнститут микробиологии НАН БеларусиInstitute of Microbiology of the National Academy of Sciences of Belarus202202082022673274284Copyright © Морозова А.Н., Охремчук А.Э., Головнева Н.А., 20222022Морозова А.Н., Охремчук А.Э., Головнева Н.А.Morozova A.N., Akhremchuk A.E., Golovnyova N.А.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/820

Проведен молекулярно-генетический анализ генома бактерий B. longum БИМ B-813Д, отличающихся высоким уровнем продукции β-галактозидазы. В геноме B. longum БИМ B-813Д выявлены гены Bgal_small_N, lacZ1, bgaB1, bgaB2, bgaB3 и lacZ2, кодирующие синтез β-галактозидаз. Предполагается, что lacZ1, bgaB2 и bgaB3, характеризующиеся высокой степенью идентичности с генами близкородственных видов бифидобактерий, кодируют ферменты, которые осуществляют специфические реакции гидролиза и трансгликозилирования углеводов. Установлено, что ферменты BgaB1, BgaB2 и BgaB3 относятся к семейству GH42 гликозил-гидролаз, а LacZ1 и LacZ2 – к семейству GH2. Детально рассмотрены области генома, кодирующие синтез β-галактозидаз В. longum БИМ В-813 Д. Сравнительный анализ локуса lacZ1 B. longum БИМ B-813 Д и сходной области генома AS143_01230 B. longum subsp. longum MC-42 показал наличие у B. longum БИМ B-813 гена транспозазы ISL3. Предполагается, что наличие инсерционной последовательности ISL3 в области lacZ1 приводит к изменению экспрессии гена и увеличению продукции β-галактозидазы у B. longum БИМ B-813Д.

The molecular-genetic analysis of the bacterial genome of the strain B. longum BIM B-813D distinguished by a high level of β-galactosidase production was performed. Genes Bgal_small_N, lacZ1, bgaB1, bgaB2 and bgaB3, and lacZ2, encoding the synthesis of β-galactosidases, were revealed in the deciphered genome. It was shown that the genes lacZ1, bgaB2, and bgaB3 characterized by an enhanced degree of similarity to the genes of closely related bifidobacterial species, presumably code for the enzymes catalyzing the specific reactions of hydrolysis and transglycosylation of carbohydrates. It was found that the enzymes BgaB1, BgaB2 and BgaB3 belong to the GH42 family of glycosyl hydrolases, whereas the enzymes LacZ1 and LacZ2 – to the GH2 family. The genome domains responsible for the synthesis of β-galactosidases in the strain B. longum BIM B-813D were studied in detail. A comparative analysis of the locus of lacZ1 in B. longum BIM B-813D and the similar genome fragment AS143_01230 from B. longum subsp. longum MC-42 detected the presence of the transposase gene ISL3 in the former strain. It was suggested that the insertion of the sequence of ISL3 in the lacZ1 locus resulted in the modified gene expression and the increased production of β-galactosidase in the strain B. longum BIM B-813D.

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The authors declare that there are no conflicts of interest present.