vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2022-67-2-229-242vestib-812Research ArticleОБЗОРЫREVIEWSПроточная цитометрия. Основы технологии и ее применение в биологииFlow cytometry. Basics of technology and its application in biologyВолотовскийИ. Д.VolotovskiI. D.

Волотовский Игорь Дмитриевич ‒ академик, доктор биологических наук, профессор, главный научный сотрудник.

Ул. Академическая, 27, 220072, Минск

Igor D. Volotovski ‒ Academician, D. Sc. (Biol.), Professor, Сhief Researcher.

27, Akademicheskaya Str., 220072, Minsk

volotovski@yahoo.comПинчукС. В.PinchukS. V.

Пинчук Сергей Владимирович ‒ кандидат биологических наук, вед. науч. сотрудник.

Ул. Академическая, 27, 220072, Минск

Sergei V. Pinchuk ‒ Ph. D. (Biol.), Leading Researcher.

27, Akademicheskaya Str., 220072, Minsk

pinchuksv@mail.ruИнститут биофизики и клеточной инженерии, НАН БеларусиInstitute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus202204052022672229242Copyright © Волотовский И.Д., Пинчук С.В., 20222022Волотовский И.Д., Пинчук С.В.Volotovski I.D., Pinchuk S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/812

В данном обзоре предпринята попытка изложить основы технологии проточной цитометрии – фундаментального метода исследования, широко применяемого в различных областях биологической науки – клеточной биологии, биофизики, биохимии, молекулярной биологии, а также в прикладной и диагностической медицине. Приведены данные о принципе работы метода, устройстве проточных цитометров и их возможностях (получение информации о структурно-функциональном состоянии клеточных популяций и сортировка клеток) и использовании в проточной цитометрии лазерных источников излучения и систем регистрации рассеянного света и флуоресценции. Кроме того, представлен широкий ассортимент различных флуорофоров и моноклональных антител. Приводятся конкретные примеры использования метода проточной цитометрии в различных экспериментах. Рассматриваются перспективы дальнейшего развития технологии.

The given review is an attempt of concentrated consideration of flow cytometry problem which is widely used as a fundamental research approach in various fields of biology like cell biology, biophysics, biochemistry and molecular biology and also in applied and diagnostic medicine. The method principle, construction of flow cytometers and their possibilities (study of structure and function state of cell populations and cell sorting), usage of lasers in flow cytometers, wide assortment of fluorophores and monoclonal antibodies. The concrete examples of flow cytometer methods in different experiments are given. The trends in development of flow cytometry are considered.

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The authors declare that there are no conflicts of interest present.