vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2022-67-2-219-228vestib-811Research ArticleСтатьиРаспространение рBS72-подобных конъюгативных плазмид среди природных бактерий рода BacillusDistribution of pBS72-like conjugative plasmids among natural bacteria of the genus Bacillushttps://orcid.org/0000-0002-4381-4499ГуриновичА. С.HurynovichA. S.

Гуринович Анастасия Сергеевна – младший научный сотрудник.

Пр. Независимости, 4, 220030, Минск

Anastasiya S. Hurynovich – Junior Researcher.

4, Nezavisimosti Ave., 220030, Minsk

nastia.gurinovich96@gmail.comСацункевичН. Е.SatsunkevichN. E.

Сацункевич Наталья Евгеньевна – младший научный сотрудник.

Пр. Независимости, 4, 220030, Минск

Natallia E. Satsunkevich – Junior Researcher.

4, Nezavisimosti Ave., 220030, Minsk

nesatsunkevich@gmail.comhttps://orcid.org/0000-0002-8623-5083ТитокМ. А.TitokM. A.

Титок Марина Алексеевна – доктор биологических наук, профессор.

Пр. Независимости, 4, 220030, Минск

Marina A. Titok – D. Sc. (Biol.), Professor.

4, Nezavisimosti Ave., 220030, Minsk

ma_titok@bsu.byБелорусский государственный университетBelarusian State University202204052022672219228Copyright © Гуринович А.С., Сацункевич Н.Е., Титок М.А., 20222022Гуринович А.С., Сацункевич Н.Е., Титок М.А.Hurynovich A.S., Satsunkevich N.E., Titok M.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/811

В результате проведенного исследования в изолированных из разных природных источников на территории Беларуси почвенных образцах, содержащих бактерии B. subtilis, с частотой 27 % (в 22 образцах из 36 исследованных) выявлены рBS72-подобные репликоны. На основе 99–100 %-ного сходства белков, ответственных за репликацию (Rep-белки) и конъюгацию (белки VirB4, VirB6, VirB11, VirD4, Mob), установлено, что pBS72подобные конъюгативные плазмиды присутствуют в клетках природных бактерий B. subtilis, циркулирующих на территории Пакистана, Китая и Нидерландов, а также в клетках бактерий B. rugosus, изолированных на территории Индии. Сходные репликоны (Rep-белки идентичны на 62 %), способные передаваться путем конъюгации (ключевые белки конъюгации идентичны на 60–80 %), обнаружены в бактериях Bacillus sp., B. licheniformis, B. paralicheniformis и B. subtilis, выделенных в США, Австралии, Китае и Южной Корее.

As a result of the research pBS72-like replicons were detected with a frequency of 27 % in soil samples isolated from various natural sources on the territory of Belarus, which contained bacteria B. subtilis (were detected in 22 samples out of 36 studied). It was established that pBS72-like conjugative plasmids are present in the cells of natural bacteria B. subtilis circulating in Pakistan, China, and the Netherlands, and are also found in bacteria B. rugosus isolated in India. The homology of proteins responsible for replication (Rep-proteins) and conjugation (VirB4, VirB6, VirB11, VirD4, Mob proteins) was 99–100 %. Similar replicons (Rep proteins are 62 % identical) capable of being transmitted by conjugation (key conjugation proteins are 60–80 % identical) were found in the bacteria Bacillus sp., B. licheniformis, B. paralicheniformis and B. subtilis, allocated in the USA, Australia, China and South Korea.

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The authors declare that there are no conflicts of interest present.