vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2022-67-2-197-205vestib-809Research ArticleСтатьиУровень свободных аминокислот и их метаболитов в микробно-тканевом комплексе тонкого кишечника и печени в условиях введения ацетата свинцаAmino acid balance of microbial-tissue complex in the small intestinal and liver under administration of lead acetatehttps://orcid.org/0000-0001-5715-7963НиколаеваИ. В.NikalayevaI. V.

Николаева Ирина Владимировна ‒ старший преподаватель.

Ул. Горького, 80, 230015, Гродно

Irina V. Nikalayeva ‒ Senior lecturer.

80, Gorky Str., 230015, Grodno

nikolayeva_i@mail.ruhttps://orcid.org/0000-0001-6829-447XШейбакВ. М.SheibakV. M.

Шейбак Владимир Михайлович ‒ доктор медицинских наук, профессор, заведующий кафедрой.

Ул. Горького, 80, 230015, Гродно

Vladimir М. Sheibak – D. Sc. (Med.), Professor, Head of the Department.

80, Gorky Str., 230015, Grodno

vsheibak@gmail.comДорошенкоЕ. М.DoroshenkoE. M.

Дорошенко Евгений Михайлович – кандидат биологических наук, доцент, ведущий научный сотрудник.

Ул. Горького, 80, 230015,  Гродно

Evgeniy M. Doroshenko ‒ Ph. D. (Biol.), Associate Professor, Leading Researcher.

80, Gorky Str., 230015, Grodno

dgi03@mail.ruГродненский государственный медицинский университетGrodno State Medical University202204052022672197205Copyright © Николаева И.В., Шейбак В.М., Дорошенко Е.М., 20222022Николаева И.В., Шейбак В.М., Дорошенко Е.М.Nikalayeva I.V., Sheibak V.M., Doroshenko E.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/809

Известно, что инициирующим событием повреждения печени при свинцовой интоксикации является воздействие ацетата свинца на микробиом кишечника и метаболический профиль энтероцитов. Цель исследования ‒ выявление зависимости концентраций свободных аминокислот и их производных в печени от аминокислотного фонда микробно-тканевого комплекса тонкого кишечника в условиях свинцовой интоксикации. В работе были использованы крысы массой 120‒140 г, которым внутрижелудочно в течение 3 недель с питьевой водой вводили ацетат свинца. Методом высокоэффективной жидкостной хроматографии определяли содержание свободных аминокислот и их азотсодержащих метаболитов в микробно-тканевом комплексе кишечника и печени. Установлено, что энтеральное поступление ацетата свинца с питьевой водой изменяет профиль свободных аминокислот и их производных в микробно-тканевом комплексе тонкого кишечника, что коррелирует с нарушением гомеостаза аминокислот в печени. Анализ корреляционных связей определяемых нами показателей указывает на существенную роль азотсодержащих метаболитов аминокислот – этаноламина и фосфоэтаноламина, таурина, а также цистатионина в обеспечении антиоксидантной защиты клеток тканей в условиях свинцовой интоксикации. Таким образом, направленность корреляционных взаимоотношений между различными азотсодержащими метаболитами микробно-тканевого комплекса и печени можно рассматривать как показатель дискоординации гомеостаза.

It is known that the initiating event of liver damage during lead intoxication is the effect of lead on the intestinal microbiome and the metabolic profile of enterocytes. The aim of the study was to reveal the dependence of the concentrations of free amino acids and their derivatives in the liver on the amino acid pool of the microbial-tissue complex of the small intestine. We used rats weighing 120‒140 g, which were injected intragastrically for three weeks with drinking water with lead acetate. Free amino acids and their nitrogen-containing metabolites in the microbial-tissue complex of the intestine and liver were determined by HPLC. Enteral intake of lead acetate with drinking water changes the profile of free amino acids and their derivatives in the microbial-tissue complex of the small intestine, which correlates with a violation of amino acid homeostasis in the liver. The analysis of the correlations of the indicators we determined indicates the essential role of nitrogen-containing metabolites of amino acids ‒ ethanolamine and phosphoethanolamine, taurine, and cystathionine. The direction of the correlation relationship between various nitrogen-containing metabolites of the microbial-tissue complex and the liver can be considered as marker of discoordination of homeostasis.

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The authors declare that there are no conflicts of interest present.