vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2022-67-2-158-171vestib-805Research ArticleСтатьиМолекулярно-генетические характеристики пациентов с сахарным диабетомMolecular-genetic characteristics of patients with diabetes mellitus. Vestsi Natsyyanal’nai akademii navuk BelarusiЛущикМ. Л.LushchykM. L.

Лущик Максим Леонидович ‒ кандидат медицинских наук, доцент.

Ул. П. Бровки, 3/3, 220013, Минск

Maxim L. Lushchyk ‒ Ph. D. (Med.), Associate Professor.

3/3, P. Browka Str., 220013, Minsk

maxim.lushchyk@gmail.comАмельяновичМ. Д.AmeliyanovichM. D.

Амельянович Максим Дмитриевич ‒ научный сотрудник.

Ул. Академическая, 27, 220072, Минск

Maxim D. Ameliyanovich – Researcher.

27, Akademicheskaya Str., 220072, Minsk

M.Ameliyanovich@igc.byТузоваА. А.TuzavaH. A.

Тузова Анна Александровна ‒ cтарший научный сотрудник.

Ул. П. Бровки, 3/3, 220013, Минск

Hanna A. Tuzava ‒ Senior Researcher.

3/3, P. Browka Str., 220013, Minsk

tuzava@yahoo.comМоссэИ. Б.MosseI. B.

Моссэ Ирма Борисовна – доктор биологических наук, профессор, главный научный сотрудник.

Ул. Академическая, 27, 220072, Минск

Irma B. Mosse ‒ D. Sс. (Biol.), Professor, Chief Researcher.

27, Akademicheskaya Str., 220072, Minsk

I.Mosse@igc.byДаниловаЛ. И.DanilovaL. I.

Данилова Лариса Ивановна ‒ доктор медицинских наук, профессор, заведующий кафедрой.

Ул. П. Бровки, 3/3, 220013, Минск

Larisa I. Danilova ‒ D. Sс. (Med.), Professor, Head of the Department.3/3, P. Browka Str., 220013, Minsk

Larisa.dan@gmail.comБелорусская медицинская академия последипломного образованияBelarusian Medical Academy of Postgraduate EducationИнститут генетики и цитологии, НАН БеларусиInstitute of Genetics and Cytology, National Academy of Sciences of Belarus202204052022672158171Copyright © Лущик М.Л., Амельянович М.Д., Тузова А.А., Моссэ И.Б., Данилова Л.И., 20222022Лущик М.Л., Амельянович М.Д., Тузова А.А., Моссэ И.Б., Данилова Л.И.Lushchyk M.L., Ameliyanovich M.D., Tuzava H.A., Mosse I.B., Danilova L.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/805

В статье рассматриваются вопросы перспективности изучения полиморфных вариантов генов рецепторов, активируемых пероксисомным пролифератором (PPARs) трех типов (PPARα, PPARδ и PPARγ) при сахарном диабете (СД) с учетом их ключевой роли в регуляции энергетического гомеостаза, продукции провоспалительных цитокинов, контроле липидных характеристик и гликемии. Основной акцент сделан на применении методов скринингового тестирования пациентов на носительство однонуклеотидных полиморфизмов (ОНП) с целью совершенствования подходов к выделению групп риска формирования СД и ассоциированных заболеваний и последующей персонификации корригирующих мероприятий. Представлены клинико-лабораторная и молекулярно-генетические характеристики групп пациентов с СД 1-го и 2-го типа, здоровых добровольцев. Изучена распространенность ОНП в генах рецепторов, активируемых PPARs, у пациентов с СД по сравнению с таковой у лиц группы контроля. Среди оцененных ОНП наиболее четкую ассоциацию с СД показал rs135551 гена PPARA. Выявлены 4 варианта гаплотипов, достоверно ассоциированных с СД 1-го и 2-го типа. Обсуждается целесообразность дальнейшего уточнения клинической и генетической гетерогенности случаев диабета у пациентов групп СД1 и СД2. Оценены перспективы разработки превентивных технологий в диабетологии с использованием результатов долговременных эпидемиологических молекулярно-генетических скринингов.

The article discusses the prospects for studying polymorphic variants of peroxisome proliferator-activated receptor genes (PPARs) of three types (PPARα, PPARδ, and PPARγ) in diabetes mellitus (DM), taking into account their key role in the regulation of energy homeostasis, production of pro-inflammatory cytokines, and lipid characteristics and glycemia control. The main emphasis is on the use of screening methods for testing patients for carriage of single nucleotide polymorphisms (SNPs) in order to improve approaches to identifying risk groups for the formation of DM and associated diseases, and subsequent personification of corrective measures. The clinical, laboratory and molecular genetic characteristics of groups of patients with type 1 and 2 diabetes, healthy volunteers are presented. The prevalence of SNPs in the genes of receptors activated by the peroxisome proliferator in patients with DM was studied in comparison with the control group. Among the evaluated SNPs of the rs135551 gene, PPARA showed the clearest association with the presence of DM. Four variants of haplotypes highly associated with DM1 and DM2 were identified. The expediency of further clarification of the clinical and genetic heterogeneity of cases of diabetes within the DM1 and DM2 groups is discussed. The prospects of this direction for the development of preventive technologies in diabetology, long-term epidemiological molecular genetic screenings are assessed.

сахарный диабетметаболические нарушениямолекулярно-генетические характеристикиоднонуклеотидный полиморфизмгаплотипактивируемые пероксисомным пролифератором рецепторыdiabetes mellitusmetabolic disordersmolecular geneticssingle nucleotide polymorphismhaplotypeperoxisome proliferator-activated receptorsИсследование выполнено в рамках задания 6.1 «Разработать ДНК-технологию выявления генетического риска эндокринных заболеваний» научнотехнической программы Союзного государства «Разработка инновационных геногеографических и геномных технологий идентификации личности и индивидуальных особенностей человека на основе изучения генофондов регионов Союзного государства»The study was carried out as part of task 6.1 “Develop DNA technology for identifying the genetic risk of endocrine diseases” of the scientific and technical program of the Union State “Development of innovative genogeographic and genomic technologies for identifying a person and individual characteristics of a person based on studying the gene pools of the regions of the Union State”ReferencesAssociation of diabetes type and chronic diabetes complications with early exit from the labour force: register-based study of people with diabetes in Finland / O. 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The authors declare that there are no conflicts of interest present.