vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2022-67-2-135-146vestib-803Research ArticleСтатьиВлияние 5-аминолевулиновой кислоты на физиолого-биохимические характеристики сортов озимой пшеницы с разным содержанием антоцианов в колеоптиляхInfluence of 5-aminolevulinic acid on physiological and biochemical characteristics of winter wheat varieties with different levels of anthocyanins in coleoptilesАверинаН. Г.AverinaN. G.

Аверина Наталия Георгиевна – доктор биологических наук, профессор, главный научный сотрудник.

Ул. Академическая, 27, 220072, Минск

Natalia G. Averina – D. Sc. (Biol.), Professor, Chief Researcher.

27, Akademicheskaya Str., 220072, Minsk

averina_ng@tyt.byСавинаС. М.SavinaS. M.

Савина Светлана Михайловна – кандидат биологических наук, старший научный сотрудник.

Ул. Академическая, 27, 220072, Минск

Sviatlana M. Savina – Ph. D. (Biol.), Senior Researcher.

27, Akademicheskaya Str., 220072, Minsk

svetlanapavluchkova@yandex.ruДремукИ. А.DremukI. A.

Дремук Ирина Александровна – кандидат биологических наук, старший научный сотрудник.

Ул. Академическая, 27, 220072, Минск

Irina A. Dremuk – Ph. D. (Biol.), Senior Researcher.

27, Akademicheskaya Str., 220072, Minsk

irinadremuk@yandex.ruЕмельяноваА. В.YemelyanavaH. V.

Емельянова Анна Викторовна – младший научный сотрудник.

Ул. Академическая, 27, 220072, Минск

Hanna V. Yemelyanava – Junior Researcher.

27, Akademicheskaya Str., 220072, Minsk

yashchuk.anna@mail.ruПрищепчикЮ. В.PryshchepchykYu. V.

Прищепчик Юлия Владимировна – младший научный сотрудник.

Ул. Академическая, 27, 220072, Минск

Yuliya V. Pryshchepchyk – Junior Researcher.

27, Akademicheskaya Str., 220072, Minsk

yuliya_prishchepchik@mail.ruУсатовА. В.UsatovА. V.

Усатов Александр Вячеславович – доктор биологических наук, профессор.

Ул. Большая Садовая, 105/42, Ростов-на-Дону

Alexandr V. Usatov – D. Sc. (Biol.), Professor.

105/42, Bolshaya Sadovaya Str., Rostovon-Don

usatova@mail.ruИнститут биофизики и клеточной инженерии, НАН БеларусиInstitute of Biophysics and Cell Engineering, National Academy of Sciences of BelarusЮжный федеральный университетSouth Federal University202204052022672135146Copyright © Аверина Н.Г., Савина С.М., Дремук И.А., Емельянова А.В., Прищепчик Ю.В., Усатов А.В., 20222022Аверина Н.Г., Савина С.М., Дремук И.А., Емельянова А.В., Прищепчик Ю.В., Усатов А.В.Averina N.G., Savina S.M., Dremuk I.A., Yemelyanava H.V., Pryshchepchyk Y.V., Usatov А.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/803

Изучен ряд физиолого-биохимических характеристик двух сортов озимой пшеницы ‒ с красными (Эт W5) и зелеными (Влади) колеоптилями. Сорт Эт W5 с высоким содержанием в колеоптилях антоцианов (в среднем 412 ± 52 мкмоль/г сырой массы), хлорофиллов (Хл) и каротиноидов и низким ‒ гема и пролина обладал высокой морозостойкостью (88 % выживших растений, подвергшихся воздействию температуры ‒8 ºС в течение 5 ч в возрасте 7 дней), а также высокой способностью к продолжению роста и развития после перенесенного охлаждения. Сорт Влади с низким содержанием антоцианов (в среднем 12,5 ± 1,2 мкмоль/г сырой массы), Хл и каротиноидов, но с более высокими, чем у сорта Эт W5, уровнями гема и пролина в колеоптилях, характеризовался низкой морозостойкостью (80 % выживших растений) и слабой способностью к продолжению роста и развития после перенесенного охлаждения. Замачивание на 2 ч семян пшеницы в растворе экзогенной АЛК (50 мг/л) перед высевом способствовало повышению всхожести семян обоих сортов, а у растений сорта Эт W5 ‒ увеличению их длины (на 10 %) и содержания антоцианов в колеоптилях (в 1,4 раза), а также повышению морозоустойчивости (до 97 %). Под воздействием АЛК морозоустойчивость растений сорта Влади возрастала до 93 %.

Physiological and biochemical characteristics of two varieties of winter wheat seedlings with red (Et W5) and green (Vladi) coleoptiles have been studied. Variety Et W5 with high content of anthocyanins (on average 412 ± 52 μmol/g fr wt), chlorophylls (Chl) and carotenoids and a low level of heme and proline in coleoptiles had high frost resistance (88 % of surviving plants exposed to temperatures of ‒8 °C for 5 h at the age of 7 days) and a high ability to continue growth and development after undergoing cooling. Variety Vladi with a low content of anthocyanins (on average 12.5 ± 1.2 μmol/g fr wt), Chl and carotenoids, but a higher level of heme and proline in coleoptiles compared to varieties Et W5 had low level of frost resistance (80 % of surviving plants) and was characterized by a low ability to continue growth and development after undergoing cooling. Soaking wheat seeds in a solution of exogenous ALA (50 mg/l) for 2 h before sowing increased the germination of seeds of both varieties, enhanced the length of plants of the Et W5 variety by 10 %, 1.4 times the content of anthocyanins in the coleoptiles of this variety plants and also increased their frost resistance up to 97 %. Under action of ALA frost resistance of Vladi seedlings increased up to 93 %.

растения озимой пшеницысорта Эт W5 и Владиколеоптилиморозоустойчивостьростадаптацияантоцианыхлорофиллы а и bгемпролин5-аминолевулиновая кислотаwinter wheat plantsvarieties Et W5 and Vladicoleoptilesfrost resistancegrowthadaptationanthocyaninschlorophylls a and bhemeproline5-aminolevulinic acidРабота выполнена при поддержке БРФФИ (договор № Б20ГРМГ-001) в рамках международного проекта «Расшифровка взаимозависимых механизмов управления биосинтезом хлорофилла и антоцианов в условиях абиотического стресса». Авторы также выражают искреннюю благодарность кандидату биологических наук, доценту С. И. Гордею – руководителю отдела озимых зерновых культур в РУП «Научно-практический центр НАН Беларуси по земледелию» за предоставление семян сортов озимой пшеницыThe work was supported by the Belarusian Republican Foundation for Fundamental Research (contract no. 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The authors declare that there are no conflicts of interest present.