vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2022-67-1-7-15vestib-791Research ArticleСтатьиБиологически активные соединения плодов и листьев Vaccinium corymbosum L.Biologically active compounds of berries and leaves of Vaccinium corymbosum L.ЧижикО. В.ChizhikO. V.

Чижик Ольга Владимировна - кандидат биологических наук, доцент, заведующий лабораторией.

ул. Сурганова, 2в, 220012, Минск.

Olga V. Chizhik - Ph. D. (Biol.), Associate Professor, Head of the Laboratory, Central Botanical Garden of the National Academy of Sciences of Belarus.

2v, Surganov Str., 220012, Minsk.

ДееваА. М.DeevaA. M.

Деева Алла Михайловна - кандидат биологических наук, старший научный сотрудник.

ул. Сурганова, 2в, 220012, Минск.

Alla M. Deeva - Ph. D. (Biol.), Senior Researcher, Central Botanical Garden of the National Academy of Sciences of Belarus.

2v, Surganov Str., 220012, Minsk.

alladzeeva@gmail.comРешетниковВ. Н.ReshetnikovV. N.

Решетников Владимир Николаевич - академик, доктор биологических наук, профессор, заведующий отделом.

ул. Сурганова, 2в, 220012, Минск.

Vladimir N. Reshetnikov - Academician, D. Sc. (Biol.), Professor, Head of the Department, Central Botanical Garden of the National Academy of Sciences of Belarus.

2v, Surganov Str., 220012, Minsk.

Центральный ботанический сад НАН БеларусиCentral Botanical Garden of the National Academy of Sciences of Belarus202202022022671715Copyright © Чижик О.В., Деева А.М., Решетников В.Н., 20222022Чижик О.В., Деева А.М., Решетников В.Н.Chizhik O.V., Deeva A.M., Reshetnikov V.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/791

Изучено суммарное содержание фенольных соединений и антоцианов в их составе, а также определен уровень антирадикальной активности (АРА) в плодах и листьях различных сортов Vaccinium corymbosum L.

Максимальное содержание фенольных соединений в плодах отмечено у сортов Blueray, Bluerose, Caroline Blue, Herbert, Jersey, Nelson. В исследованных таксонах доля антоциановых пигментов в составе фенольных соединений составляла от 0,01 до 0,8 % в зеленых плодах и от 22 до 52 % в зрелых. Сорта Earlyblue, Duke, Bluecrop, Bluegold характеризовались наибольшим количеством фенольных соединений в листьях.

Зафиксировано наличие тесной положительной корреляционной связи между уровнем АРА и содержанием вторичных метаболитов фенольной природы. Выявлены отличия в уровне накопления фенольных веществ у сортов голубики, обусловленные способом получения посадочного материала - методом in vivo или in vitro.

Установлено, что для определения сортосоответствия представителя рода Vaccinaceae наряду с генетическими паспортами можно использовать протеомный профиль растений голубики высокорослой.

Quantitative content of the sum of phenolic compounds and anthocyanins in their composition were study. The level of antiradical activity (ARA) in the berries and leaves of Vaccinium corymbosum L. was measured.

The maximum content of the sum of phenolic compounds was observed in Blueray, Bluerose, Caroline Blue, Herbert, Jersey, Nelson cultivars. In the cultivars which have been studied the quantity of anthocyanins in the composition of phenolic compounds ranged from 0.01 to 0.8 % in green berries, and from 22 to 52 % in mature ones. Earlyblue, Duke, Bluecrop, Bluegold cultivars were characterized by the greatest quantity of phenolic compounds in leaves.

The presence of a close positive correlation between the level of antiradical activity and the content of secondary metabolites of phenolic nature was determined. The differences in phenolic substances accumulation in blueberry cultivars obtained by in vivo and in vitro methods were revealed.

It was shown that the proteomic profile of the highbush blueberry plants together with genetic passports can being used to determine the cultivar's identity of Vaccinium corymbosum L.

Vaccinium corymbosum L.фенольные соединенияантоцианыантирадикальная активностьвторичные метаболитыin vivoin vitroпротеомный профильVaccinium corymbosum L.phenolic compoundsanthocyaninanti-radical activitysecond metabolitesin vivoin vitroproteomic profileРабота выполнена при финансовой поддержке Государственной программы научных исследований «Природные ресурсы и окружающая среда» (подпрограмма «Биоразнообразие, биоресурсы, экология», задание 06.02, 2021-2025 гг.).This work was financially supported by the State Program of Scientific Research “Natural resources and the environment” (subprogram “Biodiversity, biological resources, ecology”, grant 06.02, 2021-2025).ReferencesHolton, T. A. Genetics and biochemistry of anthocyanin biosynthesis / T. A. Holton, E. C. Cornish // Plant Cell. - 1995. -Vol. 7, N 7. - P. 1071-1083. https://doi.org/10.1105/tpc.7.7.1071Holton T. A., Cornish E. C. Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell, 1995, vol. 7, no. 7, pp. 1071-1083. https://doi.org/10.1105/tpc.7.7.1071Petroni, K. Recent advances on the regulation of anthocyanin synthesis in reproductive organs / K. Petroni, C. Tonelli // Plant Sci. - 2011. - Vol. 181, N 3. - P. 219-229. https://doi.org/10.1016/j.plantsci.2011.05.009Petroni K., Tonelli C. Recent advances on the regulation of anthocyanin synthesis in reproductive organs. Plant Science, 2011, vol. 181, no. 3, pp. 219-229. https://doi.org/10.1016/j.plantsci.2011.05.009Berry anthocyanins as novel antioxidants in human health and disease prevention / S. Zafra-Stone [et al.] // Mol. Nutr. Food Res. - 2007. - Vol. 51, N 6. - P. 675-683. https://doi.org/10.1002/mnfr.200700002Zafra-Stone S., Yasmin T., Bagchi M., Chatterjee A., Vinson J. A., Bagchi D. Berry anthocyanins as novel antioxidants in human health and disease prevention. Molecular Nutrition and Food Research, 2007, vol. 51, no. 6, pp. 675-683. https://doi.org/10.1002/mnfr.200700002He, J. Anthocyanins: natural colorants with health-promoting properties / J. He, M. M. Giusti // Ann. Rev. Food Sci. Technol. - 2010. - Vol. 1, N 1. - P. 163-187. https://doi.org/10.1146/annurev.food.080708.100754He J., Giusti M. M. Anthocyanins: natural colorants with health-promoting properties. Annual Review of Food Science and Technology, 2010, vol. 1, no. 1, pp. 163-187. https://doi.org/10.1146/annurev.food.080708.100754Tsuda, T. Dietary anthocyanin-rich plants: biochemical basis and recent progress in health benefits studies / T. Tsuda // Mol. Nutr. Food Res. - 2012. - Vol. 56, N 1. - P. 159-170. https://doi.org/10.1002/mnfr.201100526Tsuda T. Dietary anthocyanin-rich plants: biochemical basis and recent progress in health benefits studies. Molecular Nutrition and Food Research, 2012, vol. 56, no. 1, pp. 159-170. https://doi.org/10.1002/mnfr.201100526Wrolstada, R. E. Tracking color and pigment changes in anthocyanin products / R. E. Wrolstada, R. W. Dursta, J. Leeb // Trends Food Sci. Technol. - 2005. - Vol. 16, N 9. - P. 423-428. https://doi.org/10.1016/j.tifs.2005.03.019Wrolstada R. E., Dursta R. W., Leeb J. Tracking color and pigment changes in anthocyanin products. Trends in Food Science and Technology, 2005, vol. 16, no. 9, pp. 423-428. https://doi.org/10.1016/j.tifs.2005.03.019Готтих, М. Б. Определение качественного и количественного состава антоцианиновых пигментов в составе биологически активных добавок с помощью высокоэффективной жидкостной хроматографии (ВЭЖХ) / М. Б. Гот-тих, В. Н. Ташлицкий // РМЖ. Клин. офтальмология. - 2007. - T. 8, № 3. - С. 106-108.Gottikh M. B., Tashlitskii V. N. Determination of the qualitative and quantitative composition of anthocyanin pigments in biologically active additives using high performance liquid chromatography (HPLC). RMZh. Klinicheskaya oftal'mologiya [RMJ. Clinical ophthalmology], 2007, vol. 8, no. 3, pp. 106-108 (in Russian).Anthocyanin content and antioxidant activity of various red fruit juice / L. Jakobek [et al.] // Deutsche Lebensmittel-Rundschau. - 2007. - Vol. 103, N 2. - Р. 58-64.Jakobek L., Seruga M., Medvidovic-Kosanovic M. Anthocyanin content and antioxidant activity of various red fruit juice. Deutsche Lebensmittel-Rundschau, 2007, vol. 103, no. 2, pp. 58-64.Analysis of antioxidative phenolic compounds in artichoke (Cynara scolymus L.) / M. Wang [et al.] // J. Agricult. Food Chem. - 2003. - Vol. 51, N 3. - P. 601-608. https://doi.org/10.1021/jf020792bWang M., Simon J. E., Aviles I. F., He K., Zheng Q.-Y., Tadmor Y. Analysis of antioxidative phenolic compounds in artichoke (Cynara scolymus L.). Journal of Agricultural and Food Chemistry, 2003, vol. 51, no. 3, pp. 601-608. https://doi.org/10.1021/jf020792bГосударственная Фармакопея Республики Беларусь (ГФ РБ II). 2007. Т. 2, с. 1207 (С. 330).State Pharmacopoeia of the Republic of Belarus (SF RB II), 2007, vol. 2, р. 1207 (Р. 330).Antioxidant activity applying an improved ABTS radical cation decolorization assay / R. Re [et al.] // Free Rad. Biol. Med. - 1999. - Vol. 26, N 9/10. - Р. 1231-1237. https://doi.org/10.1016/s0891-5849(98)00315-3Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 1999, vol. 26, no. 9/10, рр. 1231-1237. https://doi.org/10.1016/s0891-5849(98)00315-3Jackson, B. P. Herbal drugs and phytopharmaceuticals: A handbook for practice on a scientific basis, edited and translated from the second German edition by Norman Grainger Bisset, London; German edition edited by Max Wichtl, Marburg. With a foreword by J. David Phillipson, Medpharm Scientific Publishers, Stuttgart, 1994. No. of pages: xvi + 566, price DM 298. ISBN 3-88763-025-4 [Book Reviews] / B. P. Jackson // Flavour Fragrance J. - 1995. - Vol. 10, N 5. - P. 337-339. https://doi.org/10.1002/ffj.2730100512Jackson B. P. Herbal drugs and phytopharmaceuticals: A handbook for practice on a scientific basis, edited and translated from the second German edition by Norman Grainger Bisset, London; German edition edited by Max Wichtl, Marburg. With a foreword by J. David Phillipson, Medpharm Scientific Publishers, Stuttgart, 1994. No. of pages: xvi + 566, price DM 298. ISBN 3-88763-025-4 [Book Reviews]. Flavour and Fragrance Journal, 1995, vol. 10, no. 5, pp. 337-339. https://doi.org/10.1002/ffj.2730100512Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage / U. K. Laemmli // Nature. - 1970. - Vol. 227, N 5259. - Р. 680-685. https://doi.org/10.1038/227680a0Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature, 1970, vol. 227, no. 5259, pp. 680-685. https://doi.org/10.1038/227680a0Рокицкий, П. Ф. Биологическая статистика / П. Ф. Рокицкий. - Минск : Вышэйш. шк., 1964. - 328 с.Rokitskii P. F. Biological statistics. Minsk, Vysheishaya shkola Publ., 1964. 328 p. (in Russian).Leaf anatomy and water stress of aseptically cultured “Pixy” plum grown under different environments / K. E. Brainerd [et al.] // HortScience. - 1981. - Vol. 16, N 2. - P. 173-175.Brainerd K. E., Fuchigami L.H., Kwiatkowski S., Clark C. S. Leaf anatomy and water stress of aseptically cultured “Pixy” plum grown under different environments. HortScience, 1981, vol. 16, no. 2, pp. 173-175.Principal phenolic phytochemicals and antioxidant activities of three chinese medicinal plants / H. Jang [et al.] // Food Chem. - 2007. - Vol. 103, N 3. - P. 749-756. https://doi.org/10.1016/j.foodchem.2006.09.026Jang H.-D., Chang K.-S., Huang Y.-S., Hsu C.-L., Lee S.-H., Su M.-S. Principal phenolic phytochemicals and antioxidant activities of three chinese medicinal plants. Food Chemistry, 2007, vol. 103, no. 3, pp. 749-756. https://doi.org/10.1016/j.foodchem.2006.09.026Душенков, В. Новая стратегия поиска природных биологически активных веществ / В. Душенков, И. Раскин // Физиология растений. - 2008. - Т. 55, № 4. - C. 624-628.Dushenkov V., Raskin I. A new search strategy for natural biologically active substances. Fiziologiya rastenii [Plant physiology], 2008, vol. 55, no. 4, pp. 624-628 (in Russian).Vascular proteomics: linking proteomic and metabolomic changes / M. Mayr [et al.] // Proteomics. - 2004. - Vol. 4, N 12. - P. 3751-3761. https://doi.org/10.1002/pmic.200400947Mayr M., Mayr U., Chung Y.-L., Yin X., Griffiths J. R., Xu Q. Vascular proteomics: linking proteomic and metabolomic changes. Proteomics, 2004, vol. 4, no. 12, pp. 3751-3761. https://doi.org/10.1002/pmic.200400947Shoot differentiation from protocorm callus cultures of Vanilla planifolia (Orchidaceae): proteomic and metabolic responses at early stage / T. L. Palama [et al.] // BMC Plant Biol. - 2010. - Vol. 10, N 1. - P. 82-101. https://doi.org/10.1186/1471-2229-10-82Palama T. L., Menard P., Fock I., Choi Y. H., Bourdon E., Govinden-Soulange J., Bahut M., Payet B., Verpoorte R., Kodja H. Shoot differentiation from protocorm callus cultures of Vanilla planifolia (Orchidaceae): proteomic and metabolic responses at early stage. BMC Plant Biology, 2010, vol. 10, no. 1, pp. 82-101. https://doi.org/10.1186/1471-2229-10-82

The authors declare that there are no conflicts of interest present.