vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2021-66-4-426-432vestib-772Research ArticleСтатьиГен яблони MD13G1109800 относится к транскрипционным факторам се- мейства Trihelix и экспрессируется в ответ на воздействие абиотических стрессовых факторовApple gene MD13G1109800 is a member of Trihelix family transcription factors and expressed in response to abiotic stressКузмицкаяП. В.KuzmitskayaP. V.

Кузмицкая Полина Викторовна – канд. биол. наук, ст. науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Polina V. Kuzmitskaya – P h. D . ( Biol.), S enior R esearcher

27, Akademicheskaya Str., 220072, Minsk

p.kuzmitskaya@igc.byКоролеваЕ. С.KaralevaK. S.

Королева Екатерина Сергеевна – мл. науч. с отрудник

ул. Академическая, 27, 220072, г. Минск

Katsiaryna S. Karaleva – Junior Researcher

27, Akademicheskaya Str., 220072, Minsk

e.koroleva@igc.byУрбановичО. Ю.UrbanovichO. Yu.

Урбанович Оксана Юрьевна – д-р биол. наук, доцент, заведующий лабораторией

ул. Академическая, 27, 220072, г. Минск

Oksana Yu. Urbanovich – D. Sc. (Biol.), Associate Professor, Head of the Laboratory

27, Akademicheskaya Str., 220072, Minsk

o.urbanovich@igc.byИнститут генетики и цитологии НАН БеларусиInstitute of Genetics and Cytology of the National Academy of Sciences of Belarus,202109112021664426432Copyright © Кузмицкая П.В., Королева Е.С., Урбанович О.Ю., 20212021Кузмицкая П.В., Королева Е.С., Урбанович О.Ю.Kuzmitskaya P.V., Karaleva K.S., Urbanovich O.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/772

В ответе растения на различные виды абиотического стресса важную роль играет семейство транскрипционных факторов Trihelix. В представленной работе методами биоиформатики в геноме яблони сорта Golden Delicious in silico был идентифицирован ген MD13G1109800, принадлежащий к этому семейству. Анализ хромосомной локализации показал, что он расположен на 13-й хромосоме и имеет четыре интрона. Кодируемый им гипотетический белок имеет длину 365 аминокислотных остатков, молекулярную массу 42097,23 Да, изоэлектрическую точку pI = 6,21. Оценка его внутриклеточной локализации показала, что он находится в ядре. Анализ промоторной области гена MD13G1109800 указывает на то, что кодируемый им белок является участником множества сигнальных путей, запускаемых как внешними, так и внутренними факторами. Уровень экспрессии гена MD13G1109800 у подвоя яблони ММ-106 возрастает при воздействии засухи, низкой и высокой температуры, засоления.

The Trihelix family of transcription factors plays an important role in the plant’s response to various abiotic stress types. In this work in apple Golden Delicious genome we identified apple gene MD13G1109800 as a member of Triheilx family in silico. Analysis of chromosomal localization showed that it is located on chromosome 13 and has four introns. The hypothetical protein encoded by it has a length of 365 amino acid residues, a molecular weight of 42097.23 Da, an isoelectric point pI = 6.21 and located in the nucleus. Analysis of the promoter region of the MD13G1109800 gene indicates that its product is a member of many signaling pathways triggered by both external and internal factors. The expression level of the MD13G1109800 gene increases under drought, low and high temperatures, as well as salinity in the MM-106 apple rootstock.

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The authors declare that there are no conflicts of interest present.