vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2021-66-3-295-301vestib-759Research ArticleСтатьиЛитий-индуцированная модификация физико-химического состояния мембранных белков и липидов в эритроцитах человекаLithium-induced modiphication of the physicochemical state of membrane proteins and lipids in human erythrocytesЗубрицкаяГ. П.ZubritskayaG. P.

Зубрицкая Галина Петровна - кандидат биологических наук, ведущий научный сотрудник.

ул. Академическая, 27, 220072, Минск.

Galina P. Zubritskaya - Ph. D. (Biol.), Leading Researcher, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.

27, Akademicheskaya Str., 220072, Minsk.

petro371@mail.ruСлобожанинаЕ. И.SlobozhaninaE. I.

Слобожанина Екатерина Ивановна - член-корреспондент, доктор биологических наук, профессор, заведующий лабораторией.

ул. Академическая, 27, 220072, Минск.

Ekaterina I. Slobozhanina - Corresponding Member, D. Sc. (Biol.), Professor, Head of the Laboratory, Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus.

27, Akademicheskaya Str., 220072, Minsk.

slobozhanina@ibp.org.byИнститут биофизики и клеточной инженерии НАН БеларусиInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus202104082021663295301Copyright © Зубрицкая Г.П., Слобожанина Е.И., 20212021Зубрицкая Г.П., Слобожанина Е.И.Zubritskaya G.P., Slobozhanina E.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/759

Изучено влияние различных концентраций сульфата лития на эритроциты человека in vitro. Показано, что воздействие солей лития в максимальных фармакологических и токсических концентрациях на клетки приводит к модификации физико-химического состояния мембраносвязанных белков и липидов. Установлено, что в эритроцитах человека, подвергшихся воздействию ионов лития, происходит снижение активности мембраносвязанных ацетилхолинэстеразы и метгемоглобинредуктазы, а также изменение микровязкости липидного бислоя мембран. Полученные результаты могут быть использованы для создания клеточной тест-системы для оценки токсичности соединений лития.

The effect of various concentrations of lithium sulfate on human erythrocytes in vitro has been studied. It has been shown that the effect of lithium salt in maximum pharmacological and toxic concentrations on cells leads to a modification of the physicochemical state of membrane-bound proteins and lipids. It was found that in human erythrocytes exposed to lithium ions, there is a decrease in the activity of membrane-bound acetylcholinesterase and methgemoglobin reductase, as well as a change in the microviscosity of the lipid bilayer of membranes. The results obtained can be used to create a cell test system for assessing the toxicity of lithium compounds.

эритроцитарная мембранасульфат литиялипофильные зондыацетилхолинэстеразаметгемоглобинредуктазамикровязкость липидовerythrocyte membranelithium sulfatelipophilic probesacetylcholinesterasemethgemoglobin reductaselipid microviscosityРабота финансирована в рамках Государственной программы научных исследований Республики Беларусь «Биотехнологии» (мероприятие 1.52, 2019-2020 гг.).The work was funded within the framework of the State Program of Scientific Research of the Republic of Belarus “Biotechnologies” (grant 1.52, 2019-2020).ReferencesCould selenium administration alleviate the disturbances of blood parameters caused by lithium administration in rats? / M. Kielczykowska [et al.] // Biol. Trace Elem. Res. - 2014. - Vol. 158, N 3. - P. 359-364. https://doi.org/10.1007/s12011-014-9952-41 Kielczykowska M., Kocot J., Kurzepa J., Lewandowska A., Zelazowska R., Musik I. Could selenium administration alleviate the disturbances of blood parameters caused by lithium administration in rats? Biological Trace Element Research, 2014, vol. 158, no. 3, pp. 359-364. https://doi.org/10.1007/s12011-014-9952-4Bekker, R. A. Lithium preparations in psychiatty, addiction medicine and neurology. Part II. Biochemical mechanisms of its action / R. A. Bekker, Yu. V. Bykov // Acta Biomed. Scientifica. - 2019. - Vol. 4, N 2. - P. 82-102. https://doi.org/10.29413/ABS.2019-4.2.13Bekker R. A., Bykov Yu. V. Lithium preparations in psychiatty, addiction medicine and neurology. Part II. Biochemical mechanisms of its action. Acta Biomedical Scientifica, 2019, vol. 4, no. 2, pp. 82-102. https://doi.org/10.29413/ABS.2019-4.2.13Нарушение эритропоэза и изменение физико-химических свойств мембран эритроцитов у пациентов с миелодиспластическими синдромами / Е. И. Слобожанина [и др.] // Морфологические основы патологии / ред. В. П. Волкова. - Новосибирск, 2015. - С. 136-157.Slobozhanina E. I., Klimkovich N. N., Zubritskaya G. P., Kozarezova T. I. Disorder of erythropoiesis and changes in the physicochemical properties of erythrocyte membranes in patients with myelodysplastic syndromes. Morfologicheskie osnovy patologii [Morphological foundations of pathology] . Novosibirsk, 2015, pp. 136-157 (in Russian).Dodge, G. T. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes / G. T. Dodge, C. Mitchell, D. J. Hanahan // Arch. Biochem. Biophys. - 1963. - Vol. 100, N 1. - P. 119-130. https://doi.org/10.1016/0003-9861(63)90042-0Dodge G. T., Mitchell C., Hanahan D. J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Archivas of Biochemistry and Biophysics, 1963, vol. 100, no. 1, pp. 119-130. https://doi.org/10.1016/0003-9861(63)90042-0Erythrocyte membrane AchE, Na, K-ATPase and Mg-ATPase activities in mothers and their premature neonates in relation to the mode of delivery / D. G. Vlachos [et al.] // Scand. J. Clin. Lab. Invest. - 2010. - Vol. 70, N 8. - P. 568-574. https://doi.org/10.3109/00365513.2010.527365.2010Vlachos D. G., Schulpis K. H., Antsaklis A., Mesogitis S., Biliatis I., Tsakiris S. Erythrocyte membrane AchE, Na, K-ATPase and Mg-ATPase activities in mothers and their premature neonates in relation to the mode of delivery. Scandinavian Journal of Clinical Laboratory Investigation, 2010, vol. 70, no. 8, pp. 568-574. https://doi.org/10.3109/00365513.2010.527365Papandreou, P. Determination of NADH2-ferricyanide oxidoreductase (cytochrome b5 reductase, diaphorase) activity of human erythrocytes by an analysis of the time-dependence of NADH2 oxidation / P. Papandreou, E. T. Rakitzis // Clin. Chim. Acta. - 1989. - Vol. 181, N 2. - P. 189-196. https://doi.org/10.1016/02009-8981(89)90187-3Papandreou P., Rakitzis E. T. Determination of NADH2-ferricyanide oxidoreductase (cytochrome b5 reductase, diaphorase) activity of human erythrocytes by an analysis of the time-dependence of NADH2 oxidation. Clinica Chimica Acta, 1989, vol. 181, no. 2, pp. 189-196. https://doi.org/10.1016/0009-8981(89)90187-32Plasma membrane fluidity measurements on whole living cells by fluorescence anisotropy of trimethylammoniumdi-phenylhexatriene / J. G Kuhry [et al.] // Biochim. Biophys. Acta. Mol. Cell Res. - 1985. - Vol. 845, N 1. - P. 60-67. https://doi.org/10.1016/0167-4889(85)90055-2Kuhry J. G., Duportail G., Bronner C., Laustriat G. Plasma membrane fluidity measurements on whole living cells by fluorescence anisotropy of trimethylammoniumdiphenylhexatriene. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1985, vol. 845, no. 1, pp. 60-67. https://doi.org/10.1016/0167-4889(85)90055-2Harris, F. M. Use of laurdan fluorescence intensity and polarization to distinguish between changes in membrane fluidity and phospholipid order / F. M. Harris, K. B. Best, J. D. Bell // Biochim. Biophys. Acta. Biomembr. - 2002. - Vol. 1565, N 1. - P. 123-128. https://doi.org/10.1016/s0005-2736(02)00514-xHarris F. M., Best K. B., Bell J. D. Use of laurdan fluorescence intensity and polarization to distinguish between changes in membrane fluidity and phospholipid order. Biochimica et Biophysica Acta (BBA) - Biomembranes, 2002, vol. 1565, no. 1, pp. 123-128. https://doi.org/10.1016/s0005-2736(02)00514-xХемореактомное моделирование аскорбата лития / И. Ю. Торшин [и др.] // Фармакокинетика и фармакодинамика. - 2016. - Т. 3. - С. 47-58.Torshin I. Yu., Sardaryan I. S., Gromova O. A., Rastashanskii V. A., Fedotova L. E. Chemoreactomic modeling of lithium ascorbate. Farmakokinetika i farmakodinamika [Pharmacokinetics and pharmacodynamics], 2016, vol. 3, pp. 47-58 (in Russian).First-episode bipolar disorder is associated with erythrocyte membrane docosahexaenoic acid deficits: dissociation from clinical response to lithium or quetiapine / R. Mc. Namara [et al.] // Psychiatry Res. - 2015. - Vol. 230, N 2. - Р. 447-453. https://doi.org/10.1016/j.psychres.2015.09.035McNamara R. K., Jandacek R., Tso P., Blom T. J., Welge J. A., Strawn J. R., Adler C. M., DelBello M. P., Strakowski S. M. First-episode bipolar disorder is associated with erythrocyte membrane docosahexaenoic acid deficits: dissociation from clinical response to lithium or quetiapine. Psychiatry Research, 2015, vol. 230, no. 2, pp. 447-453. https://doi.org/10.1016/j.psychres.2015.09.035Адаптогенные и нейропротективные свойства аскорбата лития / А. В. Пронин [и др.] // Журн. неврол. и психиатр. - 2016. - Т. 116, № 12. - C. 86-91.Pronin A. V., Gromova O. A., Sardaryan I. S., Torshin I. Yu., Stel'mashuk E. V., Ostrenko K. S., Aleksandrova O. P., Genrikhs E. E., Khaspekov L. G. Adaptogenic and neuroprotective properties of lithium ascorbate. Zhurnal nevrologii i psikhiatrii imeni C. C. Korsakova [Journal of neurology and psychiatry named after S. S. Korsakov], 2016, vol. 116, no. 12, pp. 86-91. https://doi.org/10.17116/jnevro201611612186-91 (in Russian).Can, A. Differential antidepressant-like response to lithium treatment bet-ween mouse strains: effects of sex, maternal care, and mixed genetic background / A. Can, S. C. Piantadosi, T. D. Gould // Psychopharmacology. - 2013. - Vol. 228, N 3. -P. 411-418. https://doi.org/10.1007/s00213-013-3045-5Can A., Piantadosi S. C., Gould T. D. Differential antidepressant-like response to lithium treatment bet-ween mouse strains: Effects of sex, maternal care, and mixed genetic background. Psychopharmacology, 2013, vol. 228, no. 3, pp. 411-418. https://doi.org/10.1007/s00213-013-3045-5Dynamics of hippocampal acetylcholine release during lithium-pilocarpine-induced status epilepticus in rats / M. H. Hillert [et al.] // J. Neurochem. - 2014. - Vol. 131, N 1. - Р. 42-52. https://doi.org/10.1111/jnc.12787Hillert M. H., Imran I., Zimmermann M., Lau H., Weinfurter S., Klein J. Dynamics of hippocampal acetylcholine release during lithium-pilocarpine-induced status epilepticus in rats. Journal of Neurochemistry, 2014, vol. 131, no. 1, pp. 42-52. https://doi.org/10.1111/jnc.12787Топунов, А. Ф. Множественные функциональные формы гемоглобина в организме человека: современный взгляд и практическое использование / А. Ф. Топунов, О. В. Космочевская // Биомика. - 2018. - Т. 10, № 3. - С. 251-267.Topunov A. F., Kosmochevskaya O. V. Multiple functional forms of hemoglobin in the human body: modern view and practical use. Biomika [Biomics], 2018, vol. 10, no. 3, pp. 251-267 (in Russian).Methemoglobinemia / М. Denshaw-Burke [et al.] [Electronic resource] // Medscape. - Mode of access: https://emedicine.medscape.com/article/204178-overview. - Date of access: 17.12.2020.Denshaw-Burke М., DelGiacco E., Curran A. L., Savior D. C., Kumar M. Methemoglobinemia. Medscape. Available at: https://emedicine.medscape.com/article/204178-overview (accessed 17.12.2020).Gay, H. C. Acquired methemoglobinemia associated with topical lidocaine administration: a case report / H. C. Gay, А. P. Amaral // Drug Safety - Case Reports. - 2018. - Vol. 5, N 1. - P. 15-20. https://doi.org/10.1007/s40800-018-0081-4Gay H. C., Amaral А. P. Acquired methemoglobinemia associated with topical lidocaine administration: a case report. Drug Safety - Case Reports, 2018, vol. 5, no. 1, pp. 15-20. https://doi.org/10.1007/s40800-018-0081-4Мокрушников, П. В. Методика и результаты измерения микровязкости биомембран / П. В. Мокрушников // Тр. Новосиб. гос. архитект.-строит. ун-та (Сибистрин). - 2018. - Т. 2, № 1. - C. 17-24.Mokrushnikov P. V. Technique and results of measuring the microviscosity of biomembranes. Trudy Novosibirskogo gosudarstvennogo arkhitekturno-stroitel'nogo universiteta (Sibstrin) [Proceedings of the Novosibirsk State University of Architecture and Civil Engineering (Sibistrin)], 2018, vol. 2, no. 1, pp. 17-24 (in Russian).Li, H. Erythrocyte membrane model with explicit description of the lipid bilayer and the spectrin network / H. Li, G. Lykotrafitis // Biophys. J. - 2014. - Vol. 107, N 3. - P. 642-653. https://doi.org/10.1016/j.bpj.2014.06.031Li H., Lykotrafitis G. Erythrocyte membrane model with explicit description of the lipid bilayer and the spectrin network. Biophysical Journal, 2014, vol. 107, no. 3, pp. 642-653. https://doi.org/10.1016/j.bpj.2014.06.031Ballweg, S. Control of membrane fluidity: the OLE pathway in focus/ S. Ballweg, R. Ernst // Biol. Chem. - 2017. -Vol. 398, N 2. - P. 215-228. https://doi.org/10.1515/hsz-2016-0277Ballweg S., Ernst R. Control of membrane fluidity: the OLE pathway in focus. Journal of Biological Chemistry, 2017, vol. 398, no. 2. pp. 215-228. https://doi.org/10.1515/hsz-2016-0277Bagatolli, L. Laurdan fluorescence properties in membranes: a journey from the fluorometer to the microscope / L. Bagatolli // Fluorescent Methods to Study Biological Membranes / ed. : Y. Mely, G. Duportail. - Heidelberg, 2012. - P. 3-35.Bagatolli L. Laurdan fluorescence properties in membranes:a journey from the fluorometer to the microscope. Fluorescent Methods to Study Biological Membranes. Heidelberg, 2012, pp. 3-35.Ruiz, P. Kaplan and Sadock's. Comprehensive textbook of psychiatry / P. Ruiz. - Cambridge : Cambridge Univ. Press, 2017. - 418 p.Ruiz P. Kaplan and Sadock's. Comprehensive Textbook of Psychiatry. Cambridge, Cambridge University Press, 2017. 418 p.

The authors declare that there are no conflicts of interest present.