vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2021-66-1-88-97vestib-727Research ArticleСтатьиИдентификация и молекулярная характеристика белорусских изолятов фитоплазмы яблониThe identification and molecular characteristics of Belarusian apple proliferation phytoplasma isolatesБожидайТ. Н.BazhydaiT. N.

Божидай Татьяна Николаевна — кандтдат биологических наук, старший научный сотрудник.

Ул. Ковалева, 2, 223013, а/г Самохваловичи, Минский район

Tatsiana N. Bazhydai - Ph. D. (Biol.), Senior Researcher.

2, Kovalyov Str., 223013, а/с Samokhvalovichy, Minsk Region

tanya_bozhidaj@mail.ruКолбановаЕ. В.KolbanovaE. V.

Колбанова Елена Вячеславовна — кандтдат биологических наук, доцент, заведующий лабораторией.

Ул. Ковалева, 2, 223013, а/г Самохваловичи, Минский район

Elena V. Kolbanova - Ph. D. (Biol.), Associate Professor, Head of the Laboratory.

2, Kovalyov Str., 223013, а/с Samokhvalovichy, Minsk Region

kolbanova@tut.byКухарчикН. В.KukharchykN. V.

Кухарчик Наталья Валерьевна — доктор сельскохозяйственных наук, профессор, заведующий отделом.

Ул. Ковалева, 2, 223013, а/г Самохваловичи, Минский район

Natallia V. Kukharchyk - D. Sc. (Agricult.), Professor, Head of the Department.

2, Kovalyov Str., 223013, а/с Samokhvalovichy, Minsk Region

nkykharchyk@gmail.comИнститут плодоводстваInstitute for Fruit Growing2021080220216618897Copyright © Божидай Т.Н., Колбанова Е.В., Кухарчик Н.В., 20212021Божидай Т.Н., Колбанова Е.В., Кухарчик Н.В.Bazhydai T.N., Kolbanova E.V., Kukharchyk N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/727

Для выявления фитоплазмы яблони в осенний период наиболее подходящими образцами для диагностических исследований являются корни, а при наличии ярко выраженных характерных симптомов («ведьмины метлы») можно использовать симптоматичные побеги.

Методы полимеразной цепной реакции (ПЦР) в реальном времени с праймерами Phyto-F/Phyto-R и зондом Phyto-P и гнездовой ПЦР с праймерами P1/Tint и fO1/rO1 позволяют диагностировать Candidatus Phytoplasma mali с высокой степенью чувствительности и воспроизводимости.

Сравнение нуклеотидных последовательностей белорусских изолятов с последовательностями, представленными в EMBL/GenBank, показало, что все белорусские изоляты фитоплазмы, выявленные на растениях яблони сортов Алеся, Сябрына, Память Сикоры, относятся к виду Candidatus Phytoplasma mali. Нуклеотидные последовательности помещены в международную базу данных (EMBL/GenBank) с присвоением идентификационных номеров (LR701160, LR701188, LR701436, LR701155, LR701438, LR701439, LR701440). Идентичность нуклеотидных последовательностей фрагмента 16S rRNA гена белорусских образцов Ca. P. mali варьировалась от 99,7 до 100,0 %, участка hflB гена - от 99,6 до 100,0 %.

It is known that optimal source of samples for diagnostic of apple proliferation phytoplasma in autumn is roots. In case of the occurrence of pronounced characteristic symptoms - “witches’ broom”, can be used symptomatic shoots.

Real-time PCR with primer pair Phyto-F/Phyto-R and probe Phyto-P and nested PCR with primer pair P1/Tint and fO1/ rO1 can be used to detect Candidatus Phytoplasma mali with a high degree of sensitivity and reproducibility.

Comparison of the nucleotide sequences of Belarusian isolates with the sequences presented in EMBL/GenBank showed that all Belarusian phytoplasma isolates detected on apple cultivars Alesya, Syabryna, Pamyat Sikory belong to species Candidatus Phytoplasma mali. The nucleotide sequences are placed in international database (EMBL/GenBank) with identification numbers (LR701160, LR701188, LR701436, LR701155, LR701438, LR701439, LR701440). The identity of the nucleotide sequences of region of 16S rRNA gene of Belarusian samples of Ca. P. mali ranged from 99.7 to 100.0 %, and hflB gene region ranged from 99.6 to 100.0 %.

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The authors declare that there are no conflicts of interest present.