vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2021-66-1-42-52vestib-723Research ArticleСтатьиОсобенности фотоморфогенеза Arabidopsis thaliana в условиях LED-освещения различного спектрального составаFeatures of Arabidopsis thaliana photomorphogenesis when using LED-lighting with different spectral compositionКуделинаТ. Н.KudelinaT. N.

Куделина Татьяна Николаевна - научный сотрудник.

Ул. Академическая, 27, 220072, Минск

Tatiana N. Kudelina – Researcher.

27, Akademicheskaya Str., 220072, Minsk

10tan10@mail.ruКривобокА. С.KrivobokA. S.

Кривобок Анна Святославовна - научный сотрудник.

Хорошевское шоссе, д. 76А, 123007, Москва

Anna S. Krivobok – Researcher.

76A, Khoroshevskoe highway, 123007, Moscow

nuxin@yandex.ruБибиковаТ. Н.BibikovaT. N.

Бибикова Татьяна Николаевна - кандтдат биологических наук, старший научный сотрудник.

Ленинские горы, 1-12, 119991, Москва

Tatiana N. Bibikova - Ph. D. (Biol.), Senior Researcher.

1-12, Leninskie Gory, 119991, Moscow

bibikova@mail.bio.msu.ruМолчанО. В.MolchanO. V.

Молчан Ольга Викторовна - кандтдат биологических наук, доцент, заведующий лабораторией.

Ул. Академическая, 27, 220072, Минск

Olga V. Molchan - Ph. D. (Biol.), Associate Professor, Head of the Laboratory.

27, Akademicheskaya Str., 220072, Minsk

olga_molchan@mail.ruИнститут экспериментальной ботаники имени В.Ф. Купревича, НАН БеларусиV.F. Kuprevich Institute of Experimental Botany, National Academy of Sciences of BelarusИнститут медико-биологических проблем, РАНInstitute of Biomedical Problems, Russian Academy of SciencesМосковский государственный университет им. М.В. ЛомоносоваLomonosov Moscow State University2021080220216614252Copyright © Куделина Т.Н., Кривобок А.С., Бибикова Т.Н., Молчан О.В., 20212021Куделина Т.Н., Кривобок А.С., Бибикова Т.Н., Молчан О.В.Kudelina T.N., Krivobok A.S., Bibikova T.N., Molchan O.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/723

В работе применена ранее разработанная в Институте медико-биологических проблем РАН методика выращивания растений ArabidopsisthalianaHeynh на поверхности гидратцеллюлозной пленки в стерильных условиях на гелевых средах. При этом растения полностью доступны для наблюдения, включая корневую систему, а их листья и корни равномерно освещены. С применением данной методики изучены особенности влияния света с различным соотношением физиологически значимых спектральных диапазонов на морфогенез A. thaliana. В спектральном составе LED-облучателей, содержащем все длины волн фотосинтетически активной радиации (ФАР), варьировали соотношение красного света к синему (К/С) от 1 до 5, красного к сине-зеленому (К/(С + З) от 0,7 до 4,1 и красного к дальнему красному (К/ДК) от 2,6 до 5,6. Люминесцентное освещение с соотношениями К/С, К/(С + З) и К/ДК, равными 2, 1 и 11,9 соответственно, использовали в качестве контрольного. Полученные результаты свидетельствуют о том, что рост надземной части и корневой системы, синтез хлорофилла, накопление сухого вещества A. thaliana можно стимулировать, повышая уровень красного и снижая уровень дальнего красного света, а при наличии всех других диапазонов ФАР - достигать определенного их соотношения в спектре LED-освещения. Так, вариант LED-освещения с соотношениями К/С, К/(С + З) и К/ДК, равными 4, 2, и 5,6 соответственно, был наиболее эффективен для накопления сырой и сухой массы надземной части, образования хлорофилла, а также для формирования разветвленной корневой системы. Кроме того, при LED-освещении всех вариантов у растений в среднем на 5-6 дней раньше образовывались соцветия, а их количество было больше, чем при люминесцентном освещении.

In this work we applied the technique of growing A. thaliana Heynh plants on the surface of a hydrated cellulose film under sterile conditions on the gel environment, which was previously developed at the Institute of Biomedical Problems of the Russian Academy of Sciences. Using this technique the plant, including the root system, is fully accessible for observation and its leaves and roots are evenly illuminated. Using this technique, the features of the influence of light with a different ratio of physiologically significant spectral ranges on the morphogenesis of A. thaliana were studied. In the LED- irradiators’ spectral composition which contained all photosynthetically active radiation (PAR) wavelengths we varied the ratio of red/blue (R/B) light from 1 to 5, red/(blue-green) (R/(B + G)) from 0.7 to 4.1 and red/far red (R/FR) from 2.6 to 5.6. Luminescent lighting with R/B, R/(B + G) and R/FR ratios of 2, 1 and 11.9, respectively, was used as a control. The obtained results indicate that the growth of the aerial part and the root system, the synthesis of chlorophyll, the accumulation of dry matter of A. thaliana can be stimulated by increasing the level of red and by decreasing the level of far red light (in the presence of all other parts of PAR), reaching their certain ratio in the spectrum of LED lighting. The most effective spectral composition for the accumulation of dry and wet mass of the aerial part, the synthesizing of chlorophyll as well as for the formation of a branched root system was LED-lighting with R/B, R/(B + G) and R/FR ratios of 4, 2 and 5.6 respectively. In addition, under LED illumination of all variants, plants formed peduncles on average 5-6 days earlier and their number was greater than under luminescent one.

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The authors declare that there are no conflicts of interest present.