vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2021-66-1-26-36vestib-721Research ArticleСтатьиМолекулярно-генетические маркеры для идентификации природных бактерий рода RhodococcusMolecular-genetic analysis markers for identification of Rhodococcus bacteria specieshttps://orcid.org/0000-0003-3643-1366БукляревичА. А.BukliarevichH. A.

Букляревич Анна Александровна - младший научный сотрудник.

Пр. Независимости, 4, 220030, Минск

Hanna A. Bukliarevich - Junior Researcher

4, Nezavisimosti Ave., 220030, Minsk

bukliarevich@bsu.byhttps://orcid.org/0000-0002-8623-5083ТитокМ. А.TitokM. A.

Титок Марина Алексеевна - доктор биологических наук, профессор.

Пр. Независимости, 4, 220030, Минск

Marina A. Titok - D. Sc. (Biol.), Professor

4, Nezavisimosti Ave., 220030, Minsk

ma_titok@bsu.byБелорусский государственный университетBelarusian State University2021080220216612636Copyright © Букляревич А.А., Титок М.А., 20212021Букляревич А.А., Титок М.А.Bukliarevich H.A., Titok M.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/721

Анализ генов и кодируемых ими белков GroEL бактерий рода Rhodococcus, способного деградировать широкий спектр органических и неорганических субстратов, показал, что данные детерминанты могут быть использованы в качестве молекулярно-генетических маркеров для видовой идентификации. Разработана схема, позволяющая на основании рестрикционного анализа продуктов ПЦР генов groEL с применением рестриктаз BglI, NarI, SfiI, SinI и RseI устанавливать видовую принадлежность природных бактерий рода Rhodococcus. Аннотированс) 52 локуса генома, определяющие устойчивость бактерий R. pyridinivorans 5Ap к стрессовым условиям среды (49 структурных и 4 регуляторных гена, детерминирующих синтез 23 белков теплового шока, 9 универсальных стрессовых белков, 17 цитохромов Р450). Среди изученных детерминант выявлены уникальные нуклеотидные по-следовательности плазмидного и хромосомного происхождения, кодирующие синтез белка теплового шока DnaK (1 плазмидный ген) и цитохромов Р450 (2 хромосомных и 1 плазмидный), которые могут использоваться для молекулярного типирования биотехнологического штамма R. pyridinivorans 5Ap.

It was established that groEL genes can be used as molecular genetic markers for species identification of Rhodococcus bacteria. A restriction analysis scheme was developed for the PCR products of groEL genes using restriction enzymes BglI, NarI, SfiI, SinI, and RseI to identify the species of natural Rhodococcus bacteria. Fifty-two genome loci determining the resistance to stressful environmental condition were annotated in genome of R. pyridinivorans strain 5Ap (i.e. 49 structural and 4 regulatory genes that determine the synthesis of 23 heat shock proteins, 9 universal stress proteins, 17 P450 cytochromes). The unique nucleotide sequences encoding the synthesis of the heat shock protein DnaK (1 plasmid gene) and P450 cytochromes (2 chromosomal and 1 plasmid) were found among these genes. Thus, they can be used for molecular typing of the biotechnological R. pyridinivorans strain 5Ap.

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The authors declare that there are no conflicts of interest present.