vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2020-65-4-497-509vestib-713Research ArticleОБЗОРЫREVIEWSВзаимодействие дендримеров и дендронизированных наночастиц с белкамиInteractions of dendrimers and dendronized nanoparticles with proteinsАбашкинВ. М.AbashkinV. M.

Абашкин Виктор Михайлович - младший научный сотрудник.

Ул. Академическая, 27, 220072, Минск

Viktar М. Abashkin - Junior Researcher.

27, Akademicheskaya Str., 220072, Minsk

viktar.abashkin@gmail.comТереховаМ. М.TerehovaM. M.

Терехова Мария Михайловна - младший научный сотрудник.

Ул. Академическая, 27, 220072, Минск

Maria M. Terehova - Junior Researcher.

27, Akademicheskaya Str., 220072, Minsk

maryterekhova@tut.byГалец-БуйИ. В.Halets-BuiI. V.

Галец-Буй Инесса Веславовна - кандидат биологических наук, старший научный сотрудник.

Inessa V. Halets-Bui - Ph. D. (Biol.), Senior Researcher.

27, Akademicheskaya Str., 220072, Minsk

inessahalets@gmail.comЛозниковаС. Ж.LoznikovaS. G.

Лозникова Светлана Жоржевна - кандидат биологических наук, старший научный сотрудник.

Svetlana G. Loznikova - Ph. D. (Biol.), Senior Researcher.

27, Akademicheskaya Str., 220072, Minsk

s_loznikova@mail.ruДмитрукО. Г.DzmitrukV. G.

Дмитрук Ольга Геннадьевна - кандидат биологических наук, вед.  научный сотрудник.

Volha G. Dzmitruk - Ph. D. (Biol.), Leading Researcher.

27, Akademicheskaya Str., 220072, Minsk

dmitruk.olga@gmail.comМиловскаК.MilowskaK

Миловска Катажына - доктор биологических наук, профессор, старший преподаватель.

141/143, ул. Поморска, 90-236, Лодзь

Katarzyna Milowska - D. Sc. (Biol.), Professor, Senior Lecturer.

141/143, Pomorska Str., 90-236, Lodz

katarzyna.milowska@biol.uni.lodz.plЩербинД. Г.ShcharbinD. G.

Щербин Дмитрий Григорьевич - доктор биологических наук, доцент, заведующий лабораторией.

Ул. Академическая, 27, 220072, Минск

Dzmitry G. Shcharbin - D. Sc. (Biol.), Assistant Professor, Head of the Laboratory.

27, Akademicheskaya Str., 220072, Minsk

shcharbin@gmail.comИнститут биофизики и клеточной инженерии, НАН БеларусиInstitute of Biophysics and Cell Engineering, National Academy of Sciences of BelarusЛодзинский университетUniversity of Lodz202005112020654497509Copyright © Абашкин В.М., Терехова М.М., Галец-Буй И.В., Лозникова С.Ж., Дмитрук О.Г., Миловска К., Щербин Д.Г., 20202020Абашкин В.М., Терехова М.М., Галец-Буй И.В., Лозникова С.Ж., Дмитрук О.Г., Миловска К., Щербин Д.Г.Abashkin V.M., Terehova M.M., Halets-Bui I.V., Loznikova S.G., Dzmitruk V.G., Milowska K., Shcharbin D.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/713

Дендримеры - это гиперразветвленные полимеры, принадлежащие к классу наноматериалов. Данные наноструктуры и их производные (дендроны или дендронизированные наночастицы) представляют собой мультитаргетные носители, концевые группы которых можно модифицировать для достижения различных задач, в частности таких, как повышение биосовместимости и стабильности, контролируемое высвобождение активных веществ. Расширение сферы использования дендримеров и дендример-подобных структур в биологии и медицине требует понимания основных механизмов их взаимодействия с белками - одними из основных компонентов биологических систем. В зависимости от размера, поверхностного заряда, структуры и жесткости ветвей дендримеров их взаимодействие с белками может сильно различаться. В частности, могут иметь место как электростатические взаимодействия, возникающие из-за наличия у дендримеров и белков поверхностного заряда, так и гидрофобные, за счет соответствующих полостей в структуре дендримеров. Возможно образование водородных связей. Все эти взаимодействия так или иначе могут влиять на структуру и функции белков.

В данном обзоре освещены вопросы механизмов взаимодействия дендримеров и дендронизированных наночастиц с основными белковыми макромолекулами. Рассмотрено влияние наночастиц на вторичную структуру, конформацию, динамику и функциональную активность белков. Описаны различные модели дендример-белковых взаимодействий.

Dendrimers are hyperbranched polymers belonging to the class of nanomaterials. These nanostructures and their derivatives (dendrons and dendronized nanoparticles) are multi-target nanocarriers that can be modified to achieve various tasks. For example, it is possible to increase biocompatibility and stability, to control the release of active substances. Their widespread use in biology and medicine requires an understanding of the basic mechanisms of their interaction with proteins - one of the main biological systems. The interaction of dendrimers with proteins may vary depending on the size of the ones, surface charge, structure and stiffness of the branches. Here, both electrostatic interactions arising due to the presence of dendrimers and surface charge proteins, as well as hydrophobic ones, due to the corresponding cavities in the structure of dendrimers, can manifest themselves here. The formation of hydrogen bonds is possible. All these interactions in one way or another can affect the structure and functions of proteins.

Present article discusses the mechanisms of interactions between dendrimers, dendronized nanoparticles and protein macromolecules. The effect of nanoparticles on the secondary structure, conformation, dynamics and functional activity of proteins is reviewed. The different models for dendrimer-protein interactions are described.

дендримербелокбелковая коронананоматериалыdendrimerproteinprotein coronananomaterialsДанная работа поддержана Белорусским республиканским фондом фундаментальных исследований и Госкомитетом Республики Беларусь по науке и технологиям, гранты Б19АРМГ-002, Б20СЛКГ-002; Польским агентством NAWA, грант EUROPARTNER, no. PPI/APM/2018/1/00007/U/001; Польским министерством высшего образования и науки в рамках польско-белорусского сотрудничества; Государственной программой Республики Беларусь «Инновационные биотехнологии -2020», мероприятие 43This work was supported by grants Belarusian Republican Foundation for Fundamental Research and State Committee of the Republic of Belarus for Science and Technology, grants B19ARMG-002 B20SLKG-002; Polish agency NAWA, грант EUROPARTNER, no. 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The authors declare that there are no conflicts of interest present.