vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2020-65-1-7-15vestib-644Research ArticleСтатьиВлияние этанола на продуктивность Haematococcus pluvialis, содержание фотосинтетических пигментов, активных форм кислорода и астаксантинаInfluence of ethanol on the productivity of Haematococcus pluvialis, content of photosynthetic pigments, reactive oxygen species and astaxantinАверинаН. Г.AverinaN. G.

Аверина Наталия Георгиевна – д-р биол. наук, профессор, гл. науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Nataliya G. Averina – D. Sc. (Biol.), Professor, Chief researcher

27, Akademicheskaya Str., 220072, Minsk

averina_ng@tut.byКозелН. В.KozelN. V.

Козел Николай Владимирович – канд. биол. наук, заведующий лабораторией

Nikolai V. Kozel – Ph. D. (Biol.), Head of the Laboratory

27, Akademicheskaya Str., 220072, Minsk

kozel.mikalai@gmail.comЩербаковР. А.ShcherbakovR. A.

Щербаков Ростислав Александрович – канд. биол. наук, науч. сотрудник

Rostislav A. Shcherbakov – Ph. D. (Biol.), Researcher

27, Akademicheskaya Str., 220072, Minsk

sherbakov@ibp.org.byРадюкМ. С.RadyukM. S.

Радюк Мечислав Степанович – канд. биол. наук, ст. науч. сотрудник

Mechislav S. Radyuk – Ph. D. (Biol.), Senior researcher

27, Akademicheskaya Str., 220072, Minsk

radmes@mail.ruМананкинаЕ. Е.ManankinaE. E.

Мананкина Елена Евгеньевна – канд. биол. наук, науч. сотрудник

ул. Академическая, 27, 220072, г. Минск

Elena E. Manankina – Ph. D. (Biol.), Researcher

27, Akademicheskaya Str., 220072, Minsk

room454@mail.ruГончарикР. Г.GoncharikR. G.

Гончарик Руслан Геннадьевич – мл. науч. сотрудник

Ruslan G. Goncharik – Junior researcher

27, Akademicheskaya Str., 220072, Minsk

rusgon@mail.ruШалыгоН. В.ShalygoN. V.

Шалыго Николай Владимирович – член-корреспондент, д-р биол. наук, доцент

ул. Академическая, 27, 220072, г. Минск

Nikolai V. Shalygo – Corresponding Member, D. Sc. (Biol.), Assistant Professor

27, Akademicheskaya Str., 220072, Minsk

shalygo@ibp.org.byИнститут биофизики и клеточной инженерии НАН БеларусиInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus202011022020651715Copyright © Аверина Н.Г., Козел Н.В., Щербаков Р.А., Радюк М.С., Мананкина Е.Е., Гончарик Р.Г., Шалыго Н.В., 20202020Аверина Н.Г., Козел Н.В., Щербаков Р.А., Радюк М.С., Мананкина Е.Е., Гончарик Р.Г., Шалыго Н.В.Averina N.G., Kozel N.V., Shcherbakov R.A., Radyuk M.S., Manankina E.E., Goncharik R.G., Shalygo N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/644

Изучено влияние этанола (0,1; 0,3 и 1,6 %) на содержание фотосинтетических пигментов, астаксантина и активных форм кислорода (АФК) в Haematococcus pluvialis (H. pluvialis, штамм IBCE H-17), а также на продуктивность водоросли по показателю сухой биомассы. Установлено, что в изученных концентрациях этанол стимулировал накопление сухой биомассы в течение 3, 7 и 12 сут выращивания в среднем в 2 раза. При всех используемых концентрациях этанола количество клеток увеличивалось. Так, при использовании 0,3 % этанола количество клеток гематококка возросло в 2,1; 2,5 и в 3,3 раза по сравнению с их уровнем в контрольной культуре на 3-и, 7-е и 15-е сутки выращивания. При этом отмечена тенденция к уменьшению их размеров. Этанол стимулировал также накопление фотосинтетических пигментов. Так, через 3 сут инкубации в растворе, содержащем 0,1 и 0,3 % этанола, содержание хлорофилла (Хл) а составило 142 и 130 % от контроля, Хл b – 126 и 115, лютеина – 151 и 132 % соответственно. Максимальный эффект отмечен для β-каротина – 177 и 157 % по сравнению с контролем. Стрессовые условия, создаваемые этанолом, привели к генерации АФК. В частности, через 7 сут инкубации при использовании 0,1; 0,3 и 1,6 % этанола количество АФК составило 114, 141 и 179 % от контроля, а через 12 сут инкубации – 130, 165 и 183 % соответственно. Отмечено существенное положительное влияние этанола на содержание астаксантина. Так, через 7 сут инкубации содержание астаксантина при использовании 0,1; 0,3 и 1,6 % этанола возросло в 3,6; 4,9 и 4,6 раза соответственно, а через 12 сут – в 8,6; 6,6 и 7,7 раза по сравнению с контролем. Результаты указывают на огромный потенциал использования этанола как в качестве эффективного индуктора накопления астаксантина в клетках гематококка, так и в качестве активного стимулятора продуктивности водоросли.

The effect of ethanol (0.1, 0.3, and 1.6 %) on the content of photosynthetic pigments, astaxanthin, and reactive oxygen species (ROS) in Haematococcus pluvialis (H. pluvialis, strain IBCE H-17), as well as on algae productivity, was studied in terms of dry biomass. Ethanol was found to stimulate in the studied concentrations the accumulation of dry biomass for 3, 7, and 12 days of cultivation on average 2 times. At all used ethanol concentrations, the number of cells increased. So, when using 0.3 % ethanol, the number of cells increased by 2.1; 2.5 and 3.3 times compared with the control culture on days 3, 7 and 15 of haematococcus cell growth. At the same time, a tendency toward a decrease in their size was noted. Ethanol also stimulated the accumulation of photosynthetic pigments. So, after 3 days of incubation in a solution containing 0.1 and 0.3 % ethanol, the content of chlorophyll (Chl) a was 142 and 130 % of that in the control, respectively, Chl b – 126 and 115 %, lutein – 151 and 132 %. The maximum effect was noted for β-carotene – 177 and 157 % compared with the control. The stress conditions created by ethanol led to the generation of ROS, in particular, after 7 days of incubation using 0.1; 0.3 and 1.6 % ethanol, the amount of ROS was 114, 141 and 179 % of that in the control, and after 12 days of incubation, 130, 165 and 183 %, respectively. A significant positive effect of ethanol on the content of astaxanthin was noted. So, after 7 days of incubation, the content of astaxanthin in options of 0.1; 0.3 and 1.6 % increased by 3.6; 4.9 and 4.6 times, respectively, and after 12 days – 8.6; 6.6 and 7.7 times compared with the control. The results indicate the enormous potential of using ethanol as an effective inducer of astaxanthin accumulation in haematococcus cells, as well as an active stimulator of algal productivity.

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The authors declare that there are no conflicts of interest present.