vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2019-64-3-292-301vestib-447Research ArticleСтатьиХарактеристика плазмид бактерий Bacillus pumilus, изолированных на территории БеларусиCharacteristic of plasmids of Bacillus pumilus isolated in BelarusЕвдокимоваО. B.EvdokimovaO. V.

Евдокимова Олеся Владимировна – научный сотрудник 

ул. Купревича, 2, 220141, г. Минск

Olesia V. Evdokimova – Researcher

2, Kuprevich Str., 220141, Minsk, Republic of Belarus

evdokimovalesia@gmail.comЧиндареваМ. А.ChindarevaM. A.

Чиндарева Мария Александровна – студентка 

пр. Независимости, 4, 220030, г. Минск

 

Maryja A. Chindareva – Student 

4, Nezavisimosti Ave., 220030, Minsk, Republic of Belarus

maryja.che@gmail.comВалентовичЛ. Н.ValentovichL. N.

Валентович Леонид Николаевич– кандидат биологических наук, доцент, заведующий лабораторией 

ул. Купревича, 2, 220141, г. Минск; пр. Независимости, 4, 220030, г. Минск

Leonid N. Valentovich – Ph. D. (Biol.), Assistant Professor, Head of the Laboratory 

2, Kuprevich Str., 220141, Minsk;  4, Nezavisimosti Ave., 220030, Minsk, Republic of Belarus

valentovich@mbio.bas-net.byИнститут микробиологии НАН БеларусиInstitute of Microbiology of the National Academy of Sciences of BelarusБелорусский государственный университетBelarusian State UniversityИнститут микробиологии НАН Беларуси; Белорусский государственный университетInstitute of Microbiology of the National Academy of Sciences of Belarus; Belarusian State University201916082019643292301Copyright © Евдокимова О.B., Чиндарева М.А., Валентович Л.Н., 20192019Евдокимова О.B., Чиндарева М.А., Валентович Л.Н.Evdokimova O.V., Chindareva M.A., Valentovich L.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/447

В клетках природных бактерий Bacillus pumilus выявлены плазмиды размером от 6,2 до 8,5 т. п. н., копирующиеся в соответствии с механизмом «катящегося кольца» (RCR-типа) семейства pC194. Показано, что данные внехромосомные элементы достаточно широко распространены среди бактерий B. pumilus, циркулирующих на территории Беларуси (19 штаммов из 41 исследованного содержат RCR-плазмиды), и характеризуются генетическим полиморфизмом. Наиболее часто встречаются внехромосомные генетические элементы размером 7,7 т. п. н., идентичные плазмиде pBp15.1S из энтомопатогенного штамма B. pumilus 15.1. Остальные 6 вариантов плазмид отличаются друг от друга и от известных внехромосомных генетических элементов бактерий рода Bacillus. В клетках исследованных бактерий не выявлено репликонов, подобных pLS20.

In current study plasmids (size from 6.2 to 8.5 kb) copied with the “rolling circle” mechanism (RCR type) of the pC194 family have been identified in environmental bacteria Bacillus pumilus. It is shown that these extrachromosomal elements are widely distributed in B. pumilus bacteria circulating on the territory of Belarus (19 strains from 41 contain RCRplasmids) and they are characterized by genetic polymorphism. The most common extrachromosomal genetic elements (7.7 kb size) are identical to the plasmid pBp15.1S from the entomopathogenic strain B. pumilus 15.1. The remaining 6 type of plasmids differ from each other and from the known extrachromosomal genetic elements of genus Bacillus. In the investigated bacteria no replicons similar to pLS20 were detected.

Bacillus pumilusплазмидыRCR-тип репликациитета-тип репликацииBacillus pumilusplasmidsrolling circle replicationtheta mechanism of replicationИсследование выполнено при финансовой поддержке Белорусского республиканского фонда фундаментальных исследований (Б19-110). Авторы выражают благодарность профессору М. А. Титок, доценту В. Е. Мямину, ассистентам биологического факультета БГУ Е. И. Комар (кафедра микробиологии) и Ю. Н. Горовику (кафедра молекулярной биологии), а также старшему научному сотруднику В. Н. Акимову (ИБФМ РАН, Пущино) за предоставленные штаммы бактерий.The study was carried out with the financial support of the Belarusian Republican Foundation for Fundamental Research (B19-110). The authors are grateful to Professor M. A. Titok, Assistant Professor V. Ye. Miamin, Assistants of the Biological Faculty of the BSU E. E. Komar (Department of Microbiology) and Yu. N. Gorovik (Department of Molecular Biology), as well as Senior researcher V. N. 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The authors declare that there are no conflicts of interest present.