vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2019-64-3-286-291vestib-446Research ArticleСтатьиPR-белки как маркеры устойчивости озимой пшеницы (Triticum aestivum L.) к листовым патогенамPR-proteins as markers of winter wheat (Triticum aestivum L.) resistance to leaf pathogensВязовЕ. B.ViazauY. V.

Вязов Евгений Викторович – кандидат биологических наук, научный сотрудник

ул. Академическая, 27, 220072, г. Минск

Yauhen V. Viazau – Ph. D. (Biol.), Researcher 

27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus

viazau@yahoo.comРадюкМ. С.RadyukM. S.

Радюк Мечислав Степанович – кандидат биологических наук, старший научный сотрудник

ул. Академическая, 27, 220072, г. Минск

Mechislav S. Radyuk – Ph. D. (Biol.), Senior researcher

27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus

radmes@mail.ruФилипчикЕ. А.FilipchikE. A.

Филипчик Елена Александровна – младший научный сотрудник

ул. Академическая, 27, 220072, г. Минск

Elena A. Filipchik – Junior researcher

27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus

lenafil050494@mail.ruШалыгоН. В.ShalygoN. V.

Шалыго Николай Владимирович – член-корреспондент, доктор биологических наук, заведующий лабораторией

ул. Академическая, 27, 220072, г. Минск

Nikolai V. Shalygo – Corresponding Member, D. Sc. (Biol.), Head of the Laboratory

27, Akademicheskaya Str., 220072, Minsk, Republic of Belarus

shalygo@ibp.org.byИнститут биофизики и клеточной инженерии НАН БеларусиInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus201916082019643286291Copyright © Вязов Е.B., Радюк М.С., Филипчик Е.А., Шалыго Н.В., 20192019Вязов Е.B., Радюк М.С., Филипчик Е.А., Шалыго Н.В.Viazau Y.V., Radyuk M.S., Filipchik E.A., Shalygo N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/446

С использованием ПЦР-анализа в режиме реального времени изучена конститутивная экспрессия генов PR-белков, кодирующих тауматин-подобный белок (TLP), пероксидазу класса III (TaPero), хитиназу (Chitin), глюканазу (Glucan), ингибитор протеаз (PrInh), оксалатоксидазу (OxOxid) и липид-переносящий белок (Ltp) в коллекционных сортах озимой пшеницы. Показано, что растения сортов с повышенной комплексной устойчивостью к листовым патогенам имеют более высокий конститутивный уровень экспрессии генов Chitin и PrInh (в особенности генов TLP, TaPero и Glucan) по сравнению с неустойчивыми сортами. Предлагается использовать конститутивные уровни экспрессии генов TLP, TaPero и Glucan для отбора сортообразцов озимой пшеницы с повышенной устойчивостью к комплексу листовых болезней.

Using real-time PCR analysis, the constitutive expression of PR-protein genes encoding thaumatin-like protein (TLP), peroxidase III (TaPero), chitinase (Chitin), glucanase (Glucan), protease inhibitor (PrInh), oxalate oxidase (OxOxid) and lipid transfer protein (Ltp) was studied in collection varieties of winter wheat. It has been shown that plants of varieties with increased resistance to a complex of leaf pathogens have higher constitutive expression levels of Chitin and PrInh genes, and, to a greater extent, of TLP, TaPero and Glucan genes, compared with non-resistant varieties. It is proposed to use constitutive levels of expression of TLP, TaPero and Glucan genes for the selection of winter wheat varietal samples with increased resistance to the complex of leaf diseases.

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The authors declare that there are no conflicts of interest present.