vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2019-64-2-156-168vestib-424Research ArticleСтатьиМорфологические изменения митохондрий и клеток млекопитающих, индуцируемые гипохлорной кислотойMorphological changes of mitochondria and mammalian cells, induced by hypochlorous acidЗаводникИ. Б.ZavodnikI. B.

Заводник Илья Борисович – д-р биол. наук, профессор, заведующий кафедрой.

ул. Ожешко, 22, 230023, г. Гродно.

Ilya B. Zavodnik – D. Sc. (Biol.), Professor, Head of the Department.

22, Ozheshko Str., 230023, Grodno.

zavodnik_il@mail.ruКравчукР. И.KravchukR. I.

Кравчук Римма Ивановна – канд. биол. наук, ст. науч. сотрудник.

ул. Горького, 80, г. Гродно, 230015.

Rimma I. Kravchuk – Ph. D. (Biol.), Senior researcher.

80, Gorky Str., 230015, Grodno.

cnil@grsmu.byИльичТ. В.IlyichT. V.

Ильич Татьяна Викторовна – аспирант.

ул. Ожешко, 22, 230023, г. Гродно.

Tatsiana V. Ilyich – Postgraduate student.

22, Ozheshko Str., 230023, Grodno.

Tatyana-luchic@yandex.ruЛапшинаЕ. А.LapshinaE. A.

Лапшина Елена Алексеевна – канд. биол. наук, доцент.

ул. Ожешко, 22, 230023, г. Гродно.

Elena A. Lapshina – Ph. D. (Biol.), Assistant Professor.

22, Ozheshko Str., 230023, Grodno.

Lapshina_EA@grsu.byВейкоА. Г.VejkoA. G.

Вейко Артем Геннадьевич – аспирант.

ул. Ожешко, 22, 230023, г. Гродно.

Artem G. Veiko – Postgraduate student.

22, Ozheshko Str., 230023, Grodno.

Wei93@yandex.ruЗаводникЛ. Б.ZavodnikL. B.

Заводник Лев Борисович – канд. мед. наук, доцент.

ул. Ожешко, 22, 230023, г. Гродно.

Lev B. Zavodnik – Ph. D. (Med.), Assistant Professor.

22, Ozheshko Str., 230023, Grodno.

Zavodnik_LB@grsu.byОстровскаяО. Б.AstrowskajaO. B.

Островская Оксана Борисовна – канд. биол. наук, ст. науч. сотрудник.

ул. Горького, 80, г. Гродно, 230015.

Oxana B. Astrowskaja – Ph. D. (Biol.), Senior researcher.

80, Gorky Str., 230015, Grodno.

Astrowskaja@gmail.comКурбатМ. Н.KurbatM. N.

Курбат Михаил Николаевич – канд. мед. наук, доцент, заведующий лабораторией.

ул. Горького, 80, г. Гродно, 230015.

Mihail N. Kurbat – Ph. D. (Med.), Assistant Professor, Head of the Laboratory.

80, Gorky Str., 230015, Grodno.

vwmisha@mail.ruГродненский государственный университет имени Янки Купалы.Yanka Kupala State University of Grodno.Гродненский государственный медицинский университет.State Medical University of Grodno.201916052019642156168Copyright © Заводник И.Б., Кравчук Р.И., Ильич Т.В., Лапшина Е.А., Вейко А.Г., Заводник Л.Б., Островская О.Б., Курбат М.Н., 20192019Заводник И.Б., Кравчук Р.И., Ильич Т.В., Лапшина Е.А., Вейко А.Г., Заводник Л.Б., Островская О.Б., Курбат М.Н.Zavodnik I.B., Kravchuk R.I., Ilyich T.V., Lapshina E.A., Vejko A.G., Zavodnik L.B., Astrowskaja O.B., Kurbat M.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/424

Гипохлорная кислота, HOCl, является одним из наиболее сильных биологических окислителей и важнейшим медиатором воспалительных повреждений клеток и тканей. В настоящей работе изучен характер изменения морфологии митохондрий клеток печени крыс, эритроцитов крыс и клеток линии B14 при окислительном воздействии HOCl in vitro.

Внесение HOCl (300 мкМ) в суспензию митохондрий вызывало изменения органелл, незначительно снижало среднюю суммарную длину и среднее количество крист в одной митохондрии без изменения длины одной кристы, уменьшало среднюю площадь сечения митохондрии и удлиняло профили митохондрий, увеличивая число измененных митохондрий и гетерогенность популяции. Кроме того, наблюдалась деполяризация митохондриальных мембран, скорость и степень которой определялись концентрацией HOCl. Воздействие HOCl (25–150 мкМ) в течение 60–180 с индуцировало лизис эритроцитов крыс, которому предшествовало изменение формы и размеров клеток.

Рассчитанная нами константа диссоциации HOCl с эритроцитарной мембраной составила 140 ± 25 мкМ, кооперативность процесса взаимодействия (коэффициент Хилла) – 2,1. В клетках линии B14 воздействие HOCl (100 мкМ) вызывало потерю способности сорбироваться на субстрате, формировать ассоциаты и последующее сжатие клеток. Таким образом, морфологические (и функциональные) перестройки митохондрий печени крыс вследствие воз- действия HOCl могут служить одной из причин гибели клеток в очагах воспаления. На уровне целых клеток воздействие HOCl вызывает лизис эритроцитов и глубокие повреждения клеток линии B14.

Hypochlorous acid, HOCl, is one of the most powerful biological oxidants and the most important mediator of inflammatory damage of cells and tissues. The purpose of this study was to characterize the morphological features of HOCl – induced oxidative impairment in rat liver mitochondria in vitro and to compare the processes of HOCl-induced oxidation in mitochondria, erythrocytes and B14 cells.

HOCl addition (300 μM) to mitochondrial suspension resulted in mitochondrial structural changes with a decrease in the mean total length of the crista and the average number of cristae in one mitochondria with no change in the length of one crista. There was shown a slight decrease in the average cross-sectional area of one mitochondria, mitochondrial profile elongation, an increase in the number of altered mitochondria and the heterogeneity of the population. Simultaneously we observed depolarization of the mitochondrial membrane, the rate and degree of which were determined by the concentration of HOCl. HOCl addition (25–150 μМ) induced lysis of erythrocytes for 60–180 s, which was preceded by a change in the shape and size of cells. The apparent dissociation constant for the HOCl – membrane complex was estimated to be Kd = 140 ± 25 μМ, and the Hill coefficient was to be 2.1. The B14 cell exposure to HOCl (100 μМ) led to a loss of ability to sorb on the substrate, to form associates, and to subsequent shrinkage of cells.

Therefore, HOCl caused some morphological (and functional) changes in rat liver mitochondria, which may serve as one of the causes of cell death in inflammatory foci. At the level of the whole cells, the HOCl addition induced lysis of red blood cells and deep damage to B14 cells.

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The authors declare that there are no conflicts of interest present.