vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2018-63-3-328-334vestib-381Research ArticleСтатьиПОЛИМОРФИЗМ ПРОМОТОРНОЙ ОБЛАСТИ ГЕНА TASAP-А1 В КОЛЛЕКЦИИ СОРТОВ И ЛИНИЙ ОЗИМОЙ ПШЕНИЦЫ (TRITICUM AESTIVUM L.) И ЕГО ВЛИЯНИЕ НА АГРОНОМИЧЕСКИЕ ПРИЗНАКИTHE PROMOTER REGION POLYMORPHISM OF THE TASAP-A1 GENE IN THE COLLECTION OF WINTER WHEAT (TRITICUM AESTIVUM L.) VARIETIES AND LINES AND ITS INFLUENCE ON AGRONOMIC TRAITSФоминаЕ. В.FominaE. A.

науч. сотрудник. Институт генетики и цитологии НАН Беларуси

Researcher

E.Fomina@igc.byМалышевС. В.MalyshevS. V.

ст. науч. сотрудник

Senior researcher

S.Malyshev@igc.byКулинковичС. Н.KulinkovichS. N.

канд. с.-х. наук, заведующий лабораторией

Ph. D. (Agricult.), Head of the Laboratory

wheat.npc@mail.ruУрбановичО. Ю.UrbanovichO. Yu.

д-р биол. наук, заведующий лабораторией

D. Sc. (Biol.), Head of the Laboratory

O.Urbanovich@igc.byИнститут генетики и цитологии НАН Беларуси, МинскInstitute of Genetics and Cytology of the National Academy of Sciences of Belarus, MinskНаучно-практический центр НАН Беларуси по земледелию, ЖодиноScientific-Practical Center of the National Academy of Sciences of Belarus on Agriculture, Zhodino201825082018633328334Copyright © Фомина Е.В., Малышев С.В., Кулинкович С.Н., Урбанович О.Ю., 20182018Фомина Е.В., Малышев С.В., Кулинкович С.Н., Урбанович О.Ю.Fomina E.A., Malyshev S.V., Kulinkovich S.N., Urbanovich O.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/381

В ходе исследования промоторной области гена TaSAP-А1 по аллельному составу Sap5, Sap39 и Sap2606 локусов установлено, что сорта и линии озимой пшеницы из коллекции, используемой в селекционном процессе в Республике Беларусь в 2014 г., относятся к 4 гаплотипам, при этом большинство протестированных образцов (45,6 %) принадлежит к гаплотипу II. Выявлено, что гаплотип IV способствует повышению массы тысячи зерен и урожайности на 5,2 и 9,2 %, а гаплотип I – увеличению длины главного колоса и числа колосков в главном колосе на 4,4 и 2,2 % соответственно по сравнению со средними значениями данных показателей. Также установлено, что наличие делеции в позиции –1810 п. н. оказывает положительное влияние на длину главного колоса, число колосков в главном колосе и урожайность, наличие инсерции в позиции –1637 п. н. – на длину главного колоса, а наличие делеции в данной позиции – на урожайность. Выявлено положительное влияние SNP-2606С в позиции –2606 п. н. на массу тысячи зерен, длину главного колоса и урожайность.

 

During the study of the promoter region of the TaSAP-Al gene on the allelic composition of Sap5, Sap39 and Sap2606 loci, it was shown that winter wheat varieties and lines from the collection used in the selection process in the Republic of Belarus in 2014 refer to 4 haplotypes and most of the tested samples (45.6 %) belongs to the haplotype II. It was found that the haplotype IV contributes to an increase in thousand-kernel weight and yield by 5.2 and 9.2 %, and haplotype I – an increase in spike length and total number of spikelets per spike by 4.4 and 2.2 % compared with the average values of these indicators. It was also found that the presence of deletion at position –1810 bp has a positive effect on spike length, total number of spikelets per spike and yield, the presence of insertion at position –1637 bp – spike length, and the presence of deletion in this position – on yield. The positive effect of SNP-2606C at position –2606 bp on thousand-kernel weight, spike length and yield was shown.

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The authors declare that there are no conflicts of interest present.