vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2018-63-3-298-306vestib-378Research ArticleСтатьиАНАЛИЗ МЕЖСОРТОВОГО ПОЛИМОРФИЗМА ЛЮПИНА ЖЕЛТОГО (LUPINUS LUTEUS L.) С ИСПОЛЬЗОВАНИЕМ EST-SSR И SRAP-RGA МАРКЕРОВESTIMATION OF EST-SSR AND SRAP-RGA MARKERS FOR GENOTYPING YELLOW LUPIN (LUPINUS LUTEUS L.)СысолятинЕ. Н.SysoliatinE. N.

мл. науч. сотрудник

Junior researcher

N.Anisimova@igc.byАнисимоваН. В.AnisimovaN. V.

канд. биол. наук, ст. науч. сотрудник

Ph. D. (Biol.), Senior researcher

N.Anisimova@igc.byБабакО. Г.BabakO. G.

канд. биол. наук, доцент, вед. науч. сотрудник

Ph. D. (Biol.), Associate Professor, Leading researcher

O.Babak@igc.byАнохинаВ. С.AnokhinaV. S.

канд. биол. наук, доцент

Ph. D. (Biol.), Associate Professor

anokhina@tut.byРоманчукИ. Ю.RomanchukI. Y.

науч. сотрудник

Researcher

anokhina@tut.byКильчевскийА. В.KilchevskyA. V.

академик, д-р биол. наук, профессор, заведующий лабораторией

Academician, D. Sc. (Biol.), Professor, Head of the Laboratory

Kilchev@presidium.bas-net.byИнститут генетики и цитологии НАН Беларуси, МинскInstitute of Genetics and Cytology of the National Academy of Sciences of Belarus, MinskБелорусский государственный университет, МинскBelarusian State University201825082018633298306Copyright © Сысолятин Е.Н., Анисимова Н.В., Бабак О.Г., Анохина В.С., Романчук И.Ю., Кильчевский А.В., 20182018Сысолятин Е.Н., Анисимова Н.В., Бабак О.Г., Анохина В.С., Романчук И.Ю., Кильчевский А.В.Sysoliatin E.N., Anisimova N.V., Babak O.G., Anokhina V.S., Romanchuk I.Y., Kilchevsky A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/378

Показана эффективность применения EST-SSR маркеров для дифференциации образцов люпина желтого. Отобраны EST-SSR маркеры с высокими и умеренными значениями PIC (0,64–0,49) – 2itg50945, 2itg03938, 2itg20349, 2itg14694, 2itg26293, 2itg45631, пригодные для выявления генетического полиморфизма. Установлены достоверные корреляции фрагментов EST-SSR профилей с отдельными признаками продуктивности в расщепляющихся гибридных популяциях F2 от межсортовых скрещиваний люпина желтого.

14 SRAP-RGA primer combinations and 8 EST-SSR markers were tested on the collection of 10 different yellow lupine samples. The most efficient primers for intravarietal differentiation of yellow lupine were distinguished according their parameters. Reliable correlations were determined between the presence of EST-SSR fragments and some characteristics of productivity in hybrid (F2 ) yellow lupine populations.

 

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The authors declare that there are no conflicts of interest present.