vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2018-63-3-263-275vestib-375Research ArticleСтатьиВЛИЯНИЕ NaCl НА ПРОДУКТИВНОСТЬ ВОДОРОСЛИ HAEMATOCOCCUS PLUVIALIS И СОДЕРЖАНИЕ В ЕЕ КЛЕТКАХ ФОТОСИНТЕТИЧЕСКИХ ПИГМЕНТОВ, АКТИВНЫХ ФОРМ КИСЛОРОДА И АСТАКСАНТИНАEFFECT OF NaCl ON HYEMATOCOCCUS PLUVIALIS PRODUCTIVITY AND CONTENT IN ITS CELLS OF PHOTOSYNTHETIC PIGMENTS, ACTIVE OXYGEN FORMS AND ASTAXANTINEАверинаН. Г.AverinaN. G.

д-р биол. наук, профессор, гл. науч. сотрудник

D. Sc. (Biol.), Professor, Chief researcher

averina@ibp.org.byКозелН. В.KozelN. V.

канд. биол. наук, ст. науч. сотрудник

Ph. D. (Biol.), Senior researcher

kmu@tut.byЩербаковР. А.SherbakovR. A.

канд. биол. наук, науч. сотрудник

Ph. D. (Biol.), Researcher

sherbakov@ibp.org.byРадюкМ. С.RadyukM. S.

канд. биол. наук, ст. науч. сотрудник

Ph. D. (Biol.), Senior researcher

radmes@mail.ruМананкинаЕ. Е.ManankinaE. E.

канд. биол. наук, науч. сотрудник

Ph. D. (Biol.), Researcher

room454@mail.ruГончарикР. Г.GoncharikR. G.

мл. науч. сотрудник

Junior researcher

rusgon@mail.ruШалыгоН. В.ShalygoN. V.

член-корреспондент, д-р биол. наук, доцент, заведующий лабораторией.

Corresponding Member, D. Sc. (Biol.), Assistant Professor, Head of the Laboratory

shalygo@ibp.org.byИнститут биофизики и клеточной инженерии НАН Беларуси, МинскInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Minsk201825082018633263275Copyright © Аверина Н.Г., Козел Н.В., Щербаков Р.А., Радюк М.С., Мананкина Е.Е., Гончарик Р.Г., Шалыго Н.В., 20182018Аверина Н.Г., Козел Н.В., Щербаков Р.А., Радюк М.С., Мананкина Е.Е., Гончарик Р.Г., Шалыго Н.В.Averina N.G., Kozel N.V., Sherbakov R.A., Radyuk M.S., Manankina E.E., Goncharik R.G., Shalygo N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/375

Изучено влияние NaCl (25, 50, 100, 200 и 300 мМ) на продуктивность Haematococcus pluvialis (штамм IBCE H-17) по таким показателям, как сухая биомасса, содержание белка, фотосинтетических пигментов, а также астаксантина и активных форм кислорода (АФК). Установлено, что NaCl в низких и средних концентрациях (25, 50 и 100 мМ) в среде культивирования стимулировал накопление сухой биомассы в течение первых 7 сут выращивания в среднем в 1,3 раза по сравнению с контролем (стандартной средой Рудика). Через 12 сут инкубации стимуляция составила в среднем 33 % при использовании 25 и 50 мМ соли. Содержание белка в расчете на сухой вес падало, составляя в среднем 70 % от контроля на 7-е сутки инкубации при использовании 50–300 мМ соли и 55 % на 12-е сутки при концентрации соли 100–300 мМ. При выращивании водоросли в течение 7 сут на растворах, содержащих NaCl, падало и общее содержание фотосинтетических пигментов – хлорофиллов (Хл) а и b, а также каротиноидов – неоксантина, виолаксантина, лютеина и β-каротина. Хл b оказался более устойчивым к засолению по сравнению с Хл а. Из всех пигментов наибольшее отрицательное воздействие NaCl оказывал на β-каротин. Стрессовые условия, создаваемые NaCl, приводили к генерации АФК. В частности, через 7 сут культивирования общее содержание АФК в варианте «NaCl-100» в 1,7 раза превышало таковое в контрольной культуре и в 3,0 раза было выше контроля в 12-суточной культуре. Отмечено существенное положительное влияние засоления на содержание астаксантина. Максимальный эффект наблюдали при использовании 100 мМ NaCl. Через 7 сут инкубации содержание астаксантина превышало контрольные показатели в 2,8 раза, а через 12 сут – в 20,5 раза. Количество клеток водоросли через 7 сут инкубации в варианте «NaCl-100» уменьшалось в среднем на 33 %, в то время как диаметр клеток возрастал на 29 %.

The effect of NaCl (25, 50, 100, 200 and 300 mM) on the productivity of the Haematococcus pluvialis strain IBCE H-17 on such parameters as dry biomass, content of protein, photosynthetic pigments, as well as astaxanthin and reactive oxygen species (ROS) was studied. It was found that NaCl at low and medium concentrations (25, 50 and 100 mM) in the culture medium stimulated the accumulation of dry biomass during the first 7 days of cultivation on average 1,3 times as compared to the control (Rudik’s standard medium). After 12 days of incubation, stimulation averaged 33 % using 25 and 50 mM salt. The protein content on a dry weight basis fell, averaging 70 % of the control on the 7th day of incubation with 50–300 mM salt and 55 % on the 12th day for a salt concentration of 100–300 mM. When the algae was grown for 7 days on solutions containing NaCl, the total content of photosynthetic pigments – chlorophylls (Chl) a and b as well as the carotenoids neoxanthine, violaxanthin, lutein and β-carotene decreased. Chl b was more resistant to salinity than Chl a. Of all the pigments, NaCl exerts the greatest negative effect on β-carotene. Stress conditions created by NaCl led to the generation of ROS, in particular, after 7 days of cultivation the total ROS content in the “NaCl-100” variant was 1.7 times higher than that in the control culture and 3.0 times higher than the control in the 12-day culture. A significant positive effect of salinity on the content of astaxanthin was noted. The maximum effect was observed with 100 mM NaCl. After 7 days of incubation, the content of astaxanthin exceeded the control indices by 2.8 times, and after 12 days – by 20.5 times. The number of algae cells after 7 days of incubation in the “NaCl-100” variant decreased on average by 33 %, while the cell diameter increased by 29 %. 

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The authors declare that there are no conflicts of interest present.