vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2018-63-2-146-154vestib-358Research ArticleСтатьиПРЕБРИДИНГОВАЯ ОЦЕНКА РЕКОМБИНАНТНЫХ И ИНТРОГРЕССИВНЫХ ПШЕНИЧНО-РЖАНЫХ ГИБРИДОВ ПО АЛЛЕЛЬНОМУ СОСТАВУ ГЕНОВ КОРОТКОСТЕБЕЛЬНОСТИ Rht-B1, Rht-D1, Rht8 И Ddw1PREBREEDING ASSESSMENT OF DWARFING GENES Rht-B1, Rht-D1, Rht8 AND Ddw1 ALLELIC COMPOSITION IN RECOMBINANT AND INTROGRESSIVE WHEAT-RYE HYBRIDESДубовецН. И.DubovetsN. I.

член-корреспондент, д-р биол. наук, доцент, гл. науч. сотрудник

Corresponding Member, D. Sc. (Biol.), Assistant Professor, Chief researcher

N.I.Dubovets@igc.byСычеваЕ. А.SychevaY. A.

канд. биол. наук, заместитель директора по научной работе

Ph. D. (Biol.), Deputy Director for Research

E.Sycheva@igc.byДроботН. И.DrobotN. I.

мл. науч. сотрудник

Junior researcher

N.Drobot@igc.byБондаревичЕ. Б.BondarevichY. B.

науч. сотрудник

Researcher

e.bondarevich@igc.byСоловейЛ. А.SoloveyL. A.

науч. сотрудник

Researcher

lili_solovei@mail.ruСилковаО. Г.SilkovaO. G.

канд. биол. наук, вед. науч. сотрудник

Ph. D. (Biol.), Leading researcher

silkova@bionet.nsc.ruИнститут генетики и цитологии НАН Беларуси, МинскInstitute of Genetics and Cytology of the National Academy of Sciences of Belarus, MinskИнститут цитологии и генетики Сибирского отделения РАН, НовосибирскInstitute of Cytology and Genetics of the Siberian Branch of Russian Academy of Sciences, Novosibirsk201821052018632146154Copyright © Дубовец Н.И., Сычева Е.А., Дробот Н.И., Бондаревич Е.Б., Соловей Л.А., Силкова О.Г., 20182018Дубовец Н.И., Сычева Е.А., Дробот Н.И., Бондаревич Е.Б., Соловей Л.А., Силкова О.Г.Dubovets N.I., Sycheva Y.A., Drobot N.I., Bondarevich Y.B., Solovey L.A., Silkova O.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/358

Представлены результаты анализа геномной структуры и аллельного состава генов короткостебельности Rht-B1, Rht-D1, Rht8 и Ddw1 у 33 вторичных рекомбинантных линий (ВРЛ) гексаплоидных тритикале с различными типами D(A)- и D(B)-замещений хромосом и у 8 интрогрессивных линий пшеницы с R(А)-, R(B)- и R(D)-замещениями хромосом. Установлено, что генотипы ВРЛ в подавляющем большинстве содержат аллель RhtB1b в гомозиготном состоянии и только 9 из них, так же как и все интрогрессивные линии пшеницы, являются гомозиготными по аллелю Rht-B1а, а 4 линии неоднородны по аллельному составу гена Rht-B1. У всех исследованных ВРЛ присутствовал аллель Rht8а (165 п. н.), тогда как у интрогрессивных линий выявлен аллель Rht8b (174 п. н.). Гетерозиготными по аллельному составу гена Rht-D1 (Rht-D1а/Rht-D1b) являлись 5 инрогрессивных линий пшеницы. У 3 линий наряду с гетерозиготными встречались растения, гомозиготные по аллелям Rht-D1а и Rht-D1b. Образцов, несущих ген Ddw1, не выявлено. По результатам генотипирования отобраны ВРЛ гексаплоидных тритикале с геном Rht-B1b, перспективные для использования в селекции на устойчивость к полеганию.

The results of the analysis of genomic structure and allelic composition of the dwarf genes Rht-B1, Rht-D1, Rht8 and Ddw1 in 33 secondary recombinant lines (SRL) of hexaploid triticale with various types of D(A)- and D(B)- chromosome substitutions and 8 introgressive wheat lines with R(A)-, R(B)- and R(D)-chromosome substitutions are presented. It was found that in the overwhelming majority SRL genotypes contain the Rht-B1b allele in the homozygous state. Nine SRLs and all introgressive wheat lines are homozygous by the Rht-B1a allele; 4 lines are heterogeneous in the allelic composition of the Rht-B1 gene. The Rht8a allele (165 bp) was present in all the investigated SRLs, whereas the Rht8b allele (174 bp) was detected in introgressive wheat lines. Five introgressive wheat lines were heterozygous by the allelic composition of the Rht-D1 (Rht-D1a/Rht-D1b) gene. In three lines, along with the Rht-D1a/Rht-D1b genotype, plants homozygous for the RhtD1a and Rht-D1b alleles were found. No samples bearing the Ddw1 gene were identified. Based on the genotyping results, SRL of hexaploid triticale with the Rht-B1b gene ‒ promising for use in breeding for lodging resistance ‒ were selected.

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The authors declare that there are no conflicts of interest present.