vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2018-63-2-135-145vestib-357Research ArticleСтатьиВЛИЯНИЕ ОБРАБОТКИ СЕМЯН ЯЧМЕНЯ ШТАММАМИ БАКТЕРИЙ РОДА BACILLUS НА АКТИВНОСТЬ КОМПОНЕНТОВ АНТИОКСИДАНТНОЙ СИСТЕМЫ В КОРНЯХ ПРОРОСТКОВ ПРИ СОЛЕВОМ СТРЕССЕINFLUENCE TREATMENT OF BARLEY SEEDS BY GENUS BACTERIA BACILLUS ON ACTIVITY OF COMPONENTS OF THE ANTIOXIDATIVE SYSTEM IN ROOTS OF SEEDLINGS UNDER SALT STRESSДорощукО. В.DoroshchukO. V.

канд. биол. наук, ст. науч. сотрудник

Ph. D. (Biol.), Senior researcher

doroshuk.olga@mail.ruКалацкаяЖ. Н.KalatskajaJ. N.

канд. биол. наук, вед. науч. сотрудник

Ph. D. (Biol.), Leading researcher

ЛаманН. А.LamanN. A.

академик, д-р биол. наук, заведующий отделом

Academician, D. Sc. (Biol.), Head of the Department

nikolai.laman@gmail.comФроловаТ. В.FrolovaT. V.

науч. сотрудник

Researcher

frolovatv9209@gmail.comШевцовН. А.ShevtsovN. A.

мл. науч. сотрудник

Junior researcher

frolovatv9209@gmail.comОвчинниковИ. А.OvchinnikovI. A.

лаборант

laboratory assistant

igor-1606@mail.ruИнститут экспериментальной ботаники имени В. Ф. Купревича НАН Беларуси, МинскV. F. Kuprevich Institute of Experimental Botany of the National Academy of Sciences of Belarus, Мinsk201821052018632135145Copyright © Дорощук О.В., Калацкая Ж.Н., Ламан Н.А., Фролова Т.В., Шевцов Н.А., Овчинников И.А., 20182018Дорощук О.В., Калацкая Ж.Н., Ламан Н.А., Фролова Т.В., Шевцов Н.А., Овчинников И.А.Doroshchuk O.V., Kalatskaja J.N., Laman N.A., Frolova T.V., Shevtsov N.A., Ovchinnikov I.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/357

Изучена активность компонентов антиоксидантной системы в корнях проростков ячменя в условиях солевого стресса при обработке семян пятью штаммами спорообразующих бактерий рода Bacillus. Полученные данные позволяют сделать вывод о повышении устойчивости к засолению (4 %-ный раствор хлорида натрия, 24 ч) корневой системы проростков ячменя, проявляющейся в поддержании скорости роста и развития корней при действии штаммов Bacillus amyloliquefaciens (B. am.) 78 ТМ, Bacillus subtilis (B. s.) 7 MP и B. s. М 9/6 в концентрации 105 клеток/мл. Защитное действие штамма B. am. 78 ТМ сопровождается значительной аккумуляцией пролина, а в восстановительный период – накоплением пероксида водорода и активизацией супероксиддисмутазы и пероксидазы. Протекторное действие штаммов B. s. M 9/6 и B. s. 7 MP обусловлено, на наш взгляд, значительным накоплением пероксида водорода и активацией антиоксидантных ферментов в условиях стресса, а в период адаптации – сохранением повышенного содержания перекиси в сравнении с таковым в стрессовом контроле.

 

It has been studied the activity components of the antioxidative system in barley roots to five strains bacteria Bacillus genus treatment of seeds under salt stress. The data obtained allow to conclude that the higher resistance to salinity (4 % NaCl solution, 24 h) the root system of barley seedlings, which manifests itself in maintaining the speed of growth and development of roots under the action of Bacillus amyloliquefaciens (B. am.) 78 TM, Bacillus subtilis (B. s.) 7 MP and B. s. M 9/6 at a concentration of 105 cells/ml. The protective effect of strain B. am. 78 ТМ accompanied by a significant accumulation of proline, and in the recovery period the accumulation of hydrogen peroxide and increased superoxide dismutase and peroxidase. The protective action of strains of B. s. M 9/6 and B. s. 7 MP is due, in our opinion, significant accumulation of hydrogen peroxide and activation of antioxidant enzymes under stress, and in the period of adaptation the preservation of the high content of hydrogen peroxide compared with stress control.

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The authors declare that there are no conflicts of interest present.