vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1029-8940-2018-63-1-53-63vestib-346Research ArticleСтатьиОСОБЕННОСТИ СТАБИЛИЗАЦИИ КАРИОТИПА В ПОТОМСТВЕ ОТ СКРЕЩИВАНИЯ ПШЕНИЧНО-РЖАНОЙ ЗАМЕЩЕННОЙ ЛИНИИ 1R(1A) С ДИПЛОИДНОЙ РОЖЬЮPECULIARITIES OF KARIOTYPE STABILIZATION IN THE PROGENY FROM CROSSING OF THE WHEAT-RYE SUBSTITUTED LINE 1R(1A) WITH DIPLOID RYEДубовецН. И.DubovetsN. I.

д-р биол. наук, доцент, гл. науч. сотрудник

D. Sc. (Biol.), Assistant Professor, Chief researcher

N.I.Dubovets@igc.byБондаревичЕ. Б.BondarevichY. B.

науч. сотрудник

Researcher

СоловейЛ. А.SoloveyL. A.

науч. сотрудник

Researcher

СычеваЕ. А.SychevaY. A.

канд. биол. наук, заместитель директора по научной работе

Ph. D. (Biol.), Deputy Director for Research

СилковаО. Г.SilkovaO. G.

канд. биол. наук, вед. науч. сотрудник

Ph. D. (Biol.), Leading researcher

Институт генетики и цитологии НАН Беларуси, МинскInstitute of Genetics and Cytology of the National Academy of Sciences of Belarus, MinskИнститут цитологии и генетики Сибирского отделения РАН, НовосибирскInstitute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences, Novosibirsk2018040420186315363Copyright © Дубовец Н.И., Бондаревич Е.Б., Соловей Л.А., Сычева Е.А., Силкова О.Г., 20182018Дубовец Н.И., Бондаревич Е.Б., Соловей Л.А., Сычева Е.А., Силкова О.Г.Dubovets N.I., Bondarevich Y.B., Solovey L.A., Sycheva Y.A., Silkova O.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/346

Представлены результаты сравнительного анализа процесса стабилизации кариотипов у амфигаплоидов от скрещивания диплоидной ржи с мягкой пшеницей (С29 × R) и пшенично-ржаной замещенной линией 1R(1А) (1R(1А) × R). Показано, что в потомстве от скрещивания С29 × R происходит быстрая стабилизация кариотипа на октоплоидном уровне, и уже в F3 гибриды имеют геномную структуру ААВВDDRR с небольшим уровнем анеуплоидии преимущественно по хромосомам ржи, что свойственно всем октоплоидным тритикале. В гибридном материале от скрещивания 1R(1А) × R наблюдается тенденция к элиминации из кариотипа хромосом R-генома: в F3 не обнаружено хромосом 3R, 5R 6R и 7R, в F5 остались только пары 1R и 4R. При этом пара 1R заместила пару 1А, пара 4R у одних растений присутствует как дополнительная к полному комплекту хромосом пшеницы (AABBDD) в этой гомеологичной группе, у других – отсутствует, а в потомстве отдельных растений F4 отмечено формирование межгеномных замещений 4R(4А) и 4R(4В). В целом, преобладающей тенденцией стабилизации кариотипа в материале от скрещивания 1R(1А) × R является возврат к исходной пшенично-ржаной замещенной линии. Полученные данные свидетельствуют о существенном влиянии 1R(1А)-замещения на процесс стабилизации амфигаплоидов ABDR и о возможности получения в потомстве таких скрещиваний новых типов пшенично-ржаных гибридов.

 

The results of the comparative analysis of the process of karyotype stabilization in amphihaploids from crossing diploid rye with common wheat (С29 × R) and with wheat-rye substituted line 1R(1А) (1R(1А) × R) are presented. It was shown, that in the progeny from crossing C29 × R the karyotype is rapidly stabilized at the octoploid level, and hybrids have the genomic structure of AABBDDRR already in F3. In hybrid material from 1R(1A) × R crossing there is a tendency of R-chromosome elimination from the karyotype: 3R, 5R, 6R and 7R chromosomes were not detected in F3, only 1R and 4R pairs remained in F5. In this case, the 1R pair replaced the 1A pair, 4R pair exist as an additional one to the complete wheat chromosome set (AABBDD) in this homeological group in some part of plants and as 4R(4А)- or 4R(4B)- substitution in another part of plants. In general, the predominant tendency of karyotype stabilization in the material from crossing 1R (1A) × R is to return to the original wheat-rye substituted line. The obtained data testify to the significant influence of 1R (1A)-substitution on the process of amphihaploid ABDR stabilization and on the possibility of obtaining new types of wheat-rye hybrids in such crosses.

 

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The authors declare that there are no conflicts of interest present.