vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestib-321Research ArticleСтатьиВЛИЯНИЕ 5-АМИНОЛЕВУЛИНОВОЙ КИСЛОТЫ НА ПРОДУКТИВНОСТЬ И ПИГМЕНТНЫЙ СОСТАВ ВОДОРОСЛИ HAEMATOCOCCUS PLUVIALISINFLUENCE OF 5-AMINOLEVULINIC ACID ON THE PRODUCTIVITY AND PIGMENT COMPOSITION OF ALGAE HAEMATOCOCCUS PLUVIALISАверинаН. Г.AverinaN. G.

д-р биол. наук, профессор, гл. науч. сотрудник

ул. Академиче- ская, 27, 220072 

D. Sc. (Biol.), Professor, Chief researcher

27, Akademicheskaya Str., 220072

averina@ibp.org.byЩербаковР. А.SherbakovR. A.

канд. биол. наук, науч. сотрудник

ул. Академическая, 27, 220072

Ph. D. (Biol.), Researcher

27, Akademicheskaya Str., 220072

sherbakov@ibp.org.byКозелН. В.KozelN. V.

канд. биол. наук, ст. науч. сотрудник

ул. Академическая, 27, 220072

Ph. D. (Biol.), Senior researcher

27, Akademicheskaya Str., 220072

kmu@tut.byМананкинаЕ. Е.ManankinaE. E.

канд. биол. наук, науч. сотрудник

ул. Академическая, 27, 220072

Ph. D. (Biol.), Researcher

27, Akademicheskaya Str., 220072

room454@mail.ruГончарикР. Г.GoncharikR. G.

мл. науч. сотрудник

ул. Академическая, 27, 220072

Junior researcher

27, Akademicheskaya Str., 220072

rusgon@mail.ruШалыгоН. В.ShalygoN. V.

член-корреспон- дент, д-р биол. наук, доцент, заведующий лабораторией

ул. Академическая, 27, 220072

Corresponding Member, D. Sc. (Biol.), Associate Professor, Head of the Laboratory

27, Akademicheskaya Str., 220072

shalygo@ibp.org.byИнститут биофизики и клеточной инженерии НАН Беларуси, МинскInstitute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Minsk201715122017042132Copyright © Аверина Н.Г., Щербаков Р.А., Козел Н.В., Мананкина Е.Е., Гончарик Р.Г., Шалыго Н.В., 20172017Аверина Н.Г., Щербаков Р.А., Козел Н.В., Мананкина Е.Е., Гончарик Р.Г., Шалыго Н.В.Averina N.G., Sherbakov R.A., Kozel N.V., Manankina E.E., Goncharik R.G., Shalygo N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/321

Изучено влияние экзогенной 5-аминолевулиновой кислоты (АЛК) на продуктивность водоросли Haematococcus pluvialis, оцениваемой по таким показателям, как сухой вес, количество и размер клеток, а также содержание белка и фотосинтетических пигментов. Выращивание водоросли на среде Рудика с добавлением экзогенной АЛК стимулировало накопление сухой биомассы гематококка в период активного роста водоросли по сравнению как с контрольной культурой, выращиваемой без АЛК, так и с исходной. За 2 сут инкубации гематококка с АЛК в концентрации 10 мг/л сухая биомасса выросла в среднем на 31 % по отношению к таковой в исходной культуре и на 17 % по сравнению с контролем. В вариантах с использованием АЛК отмечено дозозависимое повышение содержания клеток водоросли в среднем на 26 % и уменьшение их диаметра на 15 % по сравнению с этими показателями в контрольной культуре. В 7-суточной культуре во всех вариантах опыта при добавлении АЛК повышалось содержание фотосинтетических пигментов и белка. Так, при расчете на 1 г сухого вещества содержание хлорофиллов а, b и β-каротина при использовании всех концентраций АЛК превысило контрольные значения в среднем на 37, 37 и 58 %, а ксантофиллов – неоксантина, виолаксантина и лютеина – на 31, 30 и 47 % соответственно. При этом содержание белка возросло в среднем на 20–73 %. После 12 сут инкубации эффективность действия АЛК снижалась при сохранении отмеченной выше тенденции. При обсуждении результататов учитывалось влияние экзогенной АЛК на синтез эндогенных цитокининов и стимуляцию последними роста и развития клеток водоросли, а также на формирование пигментного аппарата фотосинтеза. 

Influence of exogenous 5-aminolevulinic acid (ALA) on the algae Haematococcus pluvialis productivity – dry weight, number and size of cells as well as proteins and photosynthetic pigment content has been studied. Growing of algae cells in Rudic’s medium with supplementation of ALA (0.5; 5; 10; 20 and 30 mg/l) stimulated accumulation of cell dry weight during active algae growth as compared with control cells as well as with initial algae culture. So, for two days of algae incubation with ALA 10 mg/l the dry weight increased by an average of 31 % in relation to initial culture and by 17 % in relation to control. A dose-dependent increase in content of cell number by an average of 26 % and decrease of cell diameter by 15 % was registered in “ALA” variants as compared with these characteristics in control. After 7 days of incubation with ALA content of the photosynthetic pigments and proteins increased. So, content of chlorophylls a, b and β-carotene per g of dry matter exceeded the control values by 37, 37 and 58 %, respectively. For xanthophylls such as neoxanthin, violaxanthin and lutein – by 31, 30 and 47 %, respectively. While the protein content on average increased by 20–73 %. After 12 days of incubation, the effectiveness of ALA decreased while maintaining the above trend. The results are discussed from the position of using exogenous ALA in the synthesis of endogenous cytokinins that stimulated the growth and development of algal cells, as well as the formation of the pigmentary apparatus of photosynthesis. 

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The authors declare that there are no conflicts of interest present.