vestibИзвестия Национальной академии наук Беларуси. Серия биологических наукProceedings of the National Academy of Sciences of Belarus, Biological Series1029-89402524-230XThe Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"vestib-308Research ArticleСтатьиАНАЛИЗ ОТДЕЛЬНЫХ ВИДОВ CLADOCERA И COPEPODA МЕЗОТРОФНОГО ОЗЕРА С ИСПОЛЬЗОВАНИЕМ PHENOM PROX-SEM/EDSANALYSIS OF INDIVIDUAL CLADOCERA AND COPEPODS FROM MESOTROPHIC LAKE USING THE PHENOM PROX-SEM/EDSФараханиШабнам Г.FarahaniShabnam G.

аспирант

Postgraduate student

Sh.farahani1986@gmail.comВойталь-ФранкевичА.Wojtal-FrankiewiczA.

профессор, директор

Professor, Director

adrianna.wojtal@biol.uni.lodz.plФранкевичП.FrankiewiczP.

профессор

Professor

franek@biol.uni.lodz.plБусеваЖ.BusevaZh.

канд. биол. наук, ст. науч. сотрудник

Ph. D. (Biol.), Senior researcher

buseva_j@mail.ruНаучно-практический центр по биоресурсам НАН БеларусиScientific and Practical Center for Bioresources of the National Academy of Sciences of BelarusЛодзинский университетUniversity of Lodz201705082017036976Copyright © Фарахани Ш.Г., Войталь-Франкевич А., Франкевич П., Бусева Ж., 20172017Фарахани Ш.Г., Войталь-Франкевич А., Франкевич П., Бусева Ж.Farahani S.G., Wojtal-Frankiewicz A., Frankiewicz P., Buseva Z.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestibio.belnauka.by/jour/article/view/308

Цель данного исследования – определение содержания основных биохимических элементов в отдельных видах зоопланктона из разных биотопов мезотрофного озера и оценка стехиометрического состава зоопланктона. Стехиометрический анализ Cladocera и Copepoda из четырех различных мест обитания в литоральной и пелагической зонах озера Обстерно (Беларусь) проведен в сентябре 2016 г. с использованием метода, основанного на рентгеновском микроанализе, Phenom Prox Scanning Electron Microscope (SEM) с элементной системой обнаружения (EDS). С помощью Phenom Prox SEM/EDS определен атомный массовый процент углерода, азота и фосфора в поверхностной области тканей карапакса зоопланктона и рассчитаны соотношения C:N, C:P, N:P для Cladocera из зарослей камыша и кубышки и для Copepoda из пелагиали, чистой литорали, камыша и кубышки. Для Cladocera содержание углерода в тканях было значительно выше в пелагиали (p < 0,01), а содержание азота и фосфора, напротив, было ниже в пелагиали (p < 0,01) по сравнению с другими средами обитания. В случае с Copepoda содержание углерода и азота в их тканях не различалось в зависимости от среды обитания. Значительные различия концентраций в зависимости от места обитания обнаружены для фосфора – его содержание было значительно выше у Copepoda из пелагиали по сравнению с копеподами из кубышки (p < 0,01). 

The stoichiometric analysis of Cladocera and Copepods from four different habitats of Obsterno mesotrophic lake in Belarus have been conducted in September 2016 using a method based on X-ray microanalysis, Phenom Prox Scanning Electron Microscope (SEM) with an elemental detection system (EDS), for measurement of atomic weight fractions of carbon, nitrogen and phosphorus elements in a designated area of zooplankton tissues. Phenom Prox SEM/EDS provided atomic weight percent of C:N, C:P, N:P ratios for Cladocera in pelagial, rush beds and nymphaea and for Copepods in pelagial, bare littoral, rush beds and nymphaea respectively. For Cladocera, the content of carbon in tissues was significantly higher in pelagial than in rush beds and nymphaea habitats (p < 0.01), opposite to nitrogen and phosphorous which contents were significantly lower in the pelagial (p < 0.01) comparing with other habitats. In the case of Copepods contents of carbon and nitrogen in their tissues did not differ between habitats. Significant among habitats differences were found for phosphorus – its content was significantly higher in Copepods from pelagial than from nymphaea habitat (p < 0.01).The purpose of this research is to determine the main biochemical elements content in zooplankton samples for different biotopes and to assess the elemental composition.

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The authors declare that there are no conflicts of interest present.